Zinc bioaccumulation and ionoregulatory impacts in Fundulus heteroclitus exposed to sublethal waterborne zinc at different salinities

Vania Lucia Loro, Lygia Nogueira, Sunita R. Nadella, Chris M. Wood

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Exposure of Fundulus heteroclitus to an environmentally relevant Zn concentration (500 μg L- 1) at different salinities (0, 3.5, 10.5, and 35 ppt) revealed the following effects: (i) plasma [Zn] doubled after exposure at 0 ppt, a response which was eliminated at 35 ppt. Tissue [Zn] also increased in gill, liver, intestine, and carcass at 0 ppt. (ii) Both branchial and intestinal Ca2+ ATPase activities decreased in response to Zn at 0 ppt and were elevated at 35 ppt. Plasma [Ca] decreased by 50% at 0 ppt and by 30% at 3.5 ppt and increased by 20% at 35 ppt. Gill [Ca] decreased by 35% at 0 ppt and increased by about 30% at all higher salinities. (iii) Branchial Na +,K+ ATPase activity decreased by 50% at 0 ppt, increased by 30% and 90% at 10.5 and 35 ppt respectively. Intestinal Na+,K + ATPase activity was reduced by 30% at 0 ppt. (iv) Plasma [Na] decreased by 30% at 0 ppt in Zn-exposed. Zn exposure also disturbed the homeostasis of tissue cations (Na+, K+, Ca++, Mg++) in a tissue-specific and salinity-dependent manner. (v) Drinking rate was not altered by Zn exposure. In toxicity tests, acute Zn lethality (96-h LC50) increased in a close to linear fashion from 9.8 mg L - 1 at 0 ppt to 75.0 mg L - 1 at 35 ppt. We conclude that sublethal Zn exposure causes pathological changes in both Ca++ and Na+ homeostases, and that increasing salinity exerts protective effects against both sublethal and lethal Zn toxicities.

Original languageEnglish
Pages (from-to)96-104
Number of pages9
JournalComparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Volume166
DOIs
StatePublished - Jan 1 2014

Fingerprint

Fundulidae
Bioaccumulation
Salinity
Zinc
Tissue
Plasmas
Toxicity
Adenosine Triphosphatases
Homeostasis
Calcium-Transporting ATPases
Acute Toxicity Tests
Liver
Cations
Drinking
Intestines
sodium-translocating ATPase

Keywords

  • Calcium homeostasis
  • Killifish
  • Salinity
  • Sodium homeostasis
  • Zinc

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

Zinc bioaccumulation and ionoregulatory impacts in Fundulus heteroclitus exposed to sublethal waterborne zinc at different salinities. / Loro, Vania Lucia; Nogueira, Lygia; Nadella, Sunita R.; Wood, Chris M.

In: Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, Vol. 166, 01.01.2014, p. 96-104.

Research output: Contribution to journalArticle

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abstract = "Exposure of Fundulus heteroclitus to an environmentally relevant Zn concentration (500 μg L- 1) at different salinities (0, 3.5, 10.5, and 35 ppt) revealed the following effects: (i) plasma [Zn] doubled after exposure at 0 ppt, a response which was eliminated at 35 ppt. Tissue [Zn] also increased in gill, liver, intestine, and carcass at 0 ppt. (ii) Both branchial and intestinal Ca2+ ATPase activities decreased in response to Zn at 0 ppt and were elevated at 35 ppt. Plasma [Ca] decreased by 50{\%} at 0 ppt and by 30{\%} at 3.5 ppt and increased by 20{\%} at 35 ppt. Gill [Ca] decreased by 35{\%} at 0 ppt and increased by about 30{\%} at all higher salinities. (iii) Branchial Na +,K+ ATPase activity decreased by 50{\%} at 0 ppt, increased by 30{\%} and 90{\%} at 10.5 and 35 ppt respectively. Intestinal Na+,K + ATPase activity was reduced by 30{\%} at 0 ppt. (iv) Plasma [Na] decreased by 30{\%} at 0 ppt in Zn-exposed. Zn exposure also disturbed the homeostasis of tissue cations (Na+, K+, Ca++, Mg++) in a tissue-specific and salinity-dependent manner. (v) Drinking rate was not altered by Zn exposure. In toxicity tests, acute Zn lethality (96-h LC50) increased in a close to linear fashion from 9.8 mg L - 1 at 0 ppt to 75.0 mg L - 1 at 35 ppt. We conclude that sublethal Zn exposure causes pathological changes in both Ca++ and Na+ homeostases, and that increasing salinity exerts protective effects against both sublethal and lethal Zn toxicities.",
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