Zinc binding to the gills of rainbow trout: The effect of long-term exposure to sublethal zinc

F. Galvez, N. Webb, C. Hogstrand, C. M. Wood

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

The effects of sublethal waterborne Zn (2.28 μmol l-1) on Zn binding kinetics to the apical gill surface were studied in juvenile rainbow trout (Oncorhynchus mykiss). Two separate radiotracer techniques were employed to ascertain this information. First, in vitro binding kinetic experiments were performed at extremely elevated zinc concentrations (up to 20 mmol l-1) to measure relatively low-affinity binding sites at the gill epithelium. There were no differences in Zn binding parameters (K(m) and B(max)) for fish sublethally exposed to Zn for 21 days and their simultaneous controls. Nevertheless, Ca did have an increased inhibitory effect on Zn binding in Zn-exposed fish suggesting that the anionic groups on the gill epithelium of these fish had been altered in some manner. Additionally, in vivo Zn binding kinetics were investigated using environmentally relevant waterborne Zn concentrations (low μmol l-1 range) to isolate high-affinity Zn binding sites {Ca transporters). No appreciable alterations in the K(m) and B(max) values for Zn binding were seen between the Zn-exposed group and its simultaneous control following 15 days of exposure. Furthermore, no significant differences in CC morphometry were observed between treatments. Despite these lack of treatment effects, there were temporal alterations in K(m) B(max) and CC fractional surface area in both groups. It is proposed that these fluctuations are controlled by hormonal factors (such as stanniocalcin), believed to play a role in Ca influx.

Original languageEnglish (US)
Pages (from-to)1089-1104
Number of pages16
JournalJournal of Fish Biology
Volume52
Issue number6
DOIs
StatePublished - Jun 1 1998

Keywords

  • Acclimation
  • Binding kinetics
  • Chloride cells
  • Rainbow trout
  • Scanning electron microscopy
  • Zinc

ASJC Scopus subject areas

  • Aquatic Science

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