Zebrafish

A Pharmacogenetic Model for Anesthesia

Victoria Bedell, Elena Buglo, Daniel Marcato, Christian Pylatiuk, Ralf Mikut, Johannes Stegmaier, Will Scudder, Maxwell Wray, Stephan L Zuchner, Uwe Strähle, Ravindra Peravali, Julia Dallman

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

General anesthetics are small molecules that interact with and effect the function of many different proteins to promote loss of consciousness, amnesia, and sometimes, analgesia. Owing to the complexity of this state transition and the transient nature of these drug/protein interactions, anesthetics can be difficult to study. The zebrafish is an emerging model for the discovery of both new genes required for the response to and side effects of anesthesia. Here we discuss the tools available to manipulate the zebrafish genome, including both genetic screens and genome engineering approaches. Additionally, there are various robust behavior assays available to study anesthetic and other drug responses. These assays are available for single-gene study or high throughput for genetic or drug discovery. Finally, we present a case study of using propofol as an anesthetic in the zebrafish. These techniques and protocols make the zebrafish a powerful model to study anesthetic mechanisms and drug discovery.

Original languageEnglish (US)
Title of host publicationChemical and Biochemical Approaches for the Study of Anesthetic Function, Part A
PublisherAcademic Press Inc.
Pages189-209
Number of pages21
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Enzymology
Volume602
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Fingerprint

Pharmacogenetics
Zebrafish
Anesthetics
Anesthesia
Genes
Drug Discovery
Assays
Genome
General Anesthetics
Amnesia
Unconsciousness
Propofol
Drug Interactions
Pharmaceutical Preparations
Analgesia
Proteins
Throughput
Molecules

Keywords

  • Anesthesia
  • Behavior
  • Genome editing
  • Propofol
  • Zebrafish

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Bedell, V., Buglo, E., Marcato, D., Pylatiuk, C., Mikut, R., Stegmaier, J., ... Dallman, J. (2018). Zebrafish: A Pharmacogenetic Model for Anesthesia. In Chemical and Biochemical Approaches for the Study of Anesthetic Function, Part A (pp. 189-209). (Methods in Enzymology; Vol. 602). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2018.02.004

Zebrafish : A Pharmacogenetic Model for Anesthesia. / Bedell, Victoria; Buglo, Elena; Marcato, Daniel; Pylatiuk, Christian; Mikut, Ralf; Stegmaier, Johannes; Scudder, Will; Wray, Maxwell; Zuchner, Stephan L; Strähle, Uwe; Peravali, Ravindra; Dallman, Julia.

Chemical and Biochemical Approaches for the Study of Anesthetic Function, Part A. Academic Press Inc., 2018. p. 189-209 (Methods in Enzymology; Vol. 602).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bedell, V, Buglo, E, Marcato, D, Pylatiuk, C, Mikut, R, Stegmaier, J, Scudder, W, Wray, M, Zuchner, SL, Strähle, U, Peravali, R & Dallman, J 2018, Zebrafish: A Pharmacogenetic Model for Anesthesia. in Chemical and Biochemical Approaches for the Study of Anesthetic Function, Part A. Methods in Enzymology, vol. 602, Academic Press Inc., pp. 189-209. https://doi.org/10.1016/bs.mie.2018.02.004
Bedell V, Buglo E, Marcato D, Pylatiuk C, Mikut R, Stegmaier J et al. Zebrafish: A Pharmacogenetic Model for Anesthesia. In Chemical and Biochemical Approaches for the Study of Anesthetic Function, Part A. Academic Press Inc. 2018. p. 189-209. (Methods in Enzymology). https://doi.org/10.1016/bs.mie.2018.02.004
Bedell, Victoria ; Buglo, Elena ; Marcato, Daniel ; Pylatiuk, Christian ; Mikut, Ralf ; Stegmaier, Johannes ; Scudder, Will ; Wray, Maxwell ; Zuchner, Stephan L ; Strähle, Uwe ; Peravali, Ravindra ; Dallman, Julia. / Zebrafish : A Pharmacogenetic Model for Anesthesia. Chemical and Biochemical Approaches for the Study of Anesthetic Function, Part A. Academic Press Inc., 2018. pp. 189-209 (Methods in Enzymology).
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