Zebrafish: A Pharmacogenetic Model for Anesthesia

Victoria Bedell, Elena Buglo, Daniel Marcato, Christian Pylatiuk, Ralf Mikut, Johannes Stegmaier, Will Scudder, Maxwell Wray, Stephan Züchner, Uwe Strähle, Ravindra Peravali, Julia E. Dallman

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

General anesthetics are small molecules that interact with and effect the function of many different proteins to promote loss of consciousness, amnesia, and sometimes, analgesia. Owing to the complexity of this state transition and the transient nature of these drug/protein interactions, anesthetics can be difficult to study. The zebrafish is an emerging model for the discovery of both new genes required for the response to and side effects of anesthesia. Here we discuss the tools available to manipulate the zebrafish genome, including both genetic screens and genome engineering approaches. Additionally, there are various robust behavior assays available to study anesthetic and other drug responses. These assays are available for single-gene study or high throughput for genetic or drug discovery. Finally, we present a case study of using propofol as an anesthetic in the zebrafish. These techniques and protocols make the zebrafish a powerful model to study anesthetic mechanisms and drug discovery.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
EditorsRoderic G. Eckenhoff, Ivan J. Dmochowski
PublisherAcademic Press Inc.
Pages189-209
Number of pages21
DOIs
StatePublished - 2018

Publication series

NameMethods in Enzymology
Volume602
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Anesthesia
  • Behavior
  • Genome editing
  • Propofol
  • Zebrafish

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Zebrafish: A Pharmacogenetic Model for Anesthesia'. Together they form a unique fingerprint.

  • Cite this

    Bedell, V., Buglo, E., Marcato, D., Pylatiuk, C., Mikut, R., Stegmaier, J., Scudder, W., Wray, M., Züchner, S., Strähle, U., Peravali, R., & Dallman, J. E. (2018). Zebrafish: A Pharmacogenetic Model for Anesthesia. In R. G. Eckenhoff, & I. J. Dmochowski (Eds.), Methods in Enzymology (pp. 189-209). (Methods in Enzymology; Vol. 602). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2018.02.004