YscB of Yersinia pestis functions as a specific chaperone for YopN

Michael W. Jackson, James B. Day, Gregory V Plano

Research output: Contribution to journalArticle

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Abstract

Following contact with a encaryotic cell, Yersinia species pathogenic for humans (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica) export and translocate a distinct set of virulence proteins (YopE, YopH, YopJ, YopM, and YpkA) from the bacterium into the eucaryotic cell. During in vitro growth at 37°C in the presence of calcium, Yop secretion is blocked; however, in the absence of calcium, Yop secretion is triggered. Yop secretion occurs via a plasmid-encoded type III, or 'contact-dependent,' secretion system. The secreted YopN (also known as LcrE), TyeA, and LcrG proteins are necessary to prevent Yop secretion in the presence of calcium and prior to contact with a eucaryotic cell. In this paper we characterize the role of the yscB gene product in the regulation of Yop secretion in Y. pestis. A yscB deletion mutant secreted YopM and V antigen both in the presence and in the absence of calcium; however, the export of YopN was specifically reduced in this strain. Complementation with a functional copy of yscB in trans completely restored the wild-type secretion phenotype for YopM, YopN, and V antigen. The YscB amino acid sequence showed significant similarities to those of SycE and SycH, the specific Yop chaperones for YopE and YopH, respectively. Protein cross-linking and immunoprecipitation studies demonstrated a specific interaction between YscB and YopN. In-frame deletions in yopN eliminating the coding region for amino acids 51 to 85 or 6 to 100 prevented the interaction of YopN with YscB. Taken together, these results indicate that YscB functions as a specific chaperone for YopN in Y. pestis.

Original languageEnglish
Pages (from-to)4912-4921
Number of pages10
JournalJournal of Bacteriology
Volume180
Issue number18
StatePublished - Sep 1 1998

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Yersinia pestis
Calcium
Antigens
Yersinia
Proteins
Immunoprecipitation
Virulence
Amino Acid Sequence
Plasmids
Bacteria
Phenotype
Amino Acids
Growth
Genes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

YscB of Yersinia pestis functions as a specific chaperone for YopN. / Jackson, Michael W.; Day, James B.; Plano, Gregory V.

In: Journal of Bacteriology, Vol. 180, No. 18, 01.09.1998, p. 4912-4921.

Research output: Contribution to journalArticle

Jackson, Michael W. ; Day, James B. ; Plano, Gregory V. / YscB of Yersinia pestis functions as a specific chaperone for YopN. In: Journal of Bacteriology. 1998 ; Vol. 180, No. 18. pp. 4912-4921.
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