Yersinia pestis uses the Ail outer membrane protein to recruit vitronectin

Sara Schesser Bartra, Yi Ding, L. Miya Fujimoto, Joshua G. Ring, Vishal Jain, Sanjay Ram, Francesca M. Marassi, Gregory V Plano

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Yersinia pestis, the agent of plague, requires the Ail (attachment invasion locus) outer membrane protein to survive in the blood and tissues of its mammalian hosts. Ail is important for both attachment to host cells and for resistance to complement-dependent bacteriolysis. Previous studies have shown that Ail interacts with components of the extracellular matrix, including fibronectin, laminin and heparan sulfate proteoglycans, and with the complement inhibitor C4b-binding protein. Here, we demonstrate that Ail-expressing Y. pestis strains bind vitronectin – a host protein with functions in cell attachment, fibrinolysis and inhibition of the complement system. The Ail-dependent recruitment of vitronectin resulted in efficient cleavage of vitronectin by the outer membrane Pla (plasminogen activator protease). Escherichia coli DH5α expressing Y. pestis Ail bound vitronectin, but not heat-treated vitronectin. The ability of Ail to directly bind vitronectin was demonstrated by ELISA using purified refolded Ail in nanodiscs.

Original languageEnglish (US)
Pages (from-to)2174-2183
Number of pages10
JournalMicrobiology (United Kingdom)
Volume161
Issue number11
DOIs
StatePublished - Nov 1 2015

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Yersinia pestis
Vitronectin
Membrane Proteins
Complement Inactivating Agents
Bacteriolysis
Complement C4b-Binding Protein
Heparan Sulfate Proteoglycans
Plague
Plasminogen Activators
Laminin
Fibrinolysis
Fibronectins
Extracellular Matrix
Peptide Hydrolases
Hot Temperature
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Membranes
Proteins

ASJC Scopus subject areas

  • Microbiology

Cite this

Bartra, S. S., Ding, Y., Fujimoto, L. M., Ring, J. G., Jain, V., Ram, S., ... Plano, G. V. (2015). Yersinia pestis uses the Ail outer membrane protein to recruit vitronectin. Microbiology (United Kingdom), 161(11), 2174-2183. https://doi.org/10.1099/mic.0.000179

Yersinia pestis uses the Ail outer membrane protein to recruit vitronectin. / Bartra, Sara Schesser; Ding, Yi; Fujimoto, L. Miya; Ring, Joshua G.; Jain, Vishal; Ram, Sanjay; Marassi, Francesca M.; Plano, Gregory V.

In: Microbiology (United Kingdom), Vol. 161, No. 11, 01.11.2015, p. 2174-2183.

Research output: Contribution to journalArticle

Bartra, SS, Ding, Y, Fujimoto, LM, Ring, JG, Jain, V, Ram, S, Marassi, FM & Plano, GV 2015, 'Yersinia pestis uses the Ail outer membrane protein to recruit vitronectin', Microbiology (United Kingdom), vol. 161, no. 11, pp. 2174-2183. https://doi.org/10.1099/mic.0.000179
Bartra SS, Ding Y, Fujimoto LM, Ring JG, Jain V, Ram S et al. Yersinia pestis uses the Ail outer membrane protein to recruit vitronectin. Microbiology (United Kingdom). 2015 Nov 1;161(11):2174-2183. https://doi.org/10.1099/mic.0.000179
Bartra, Sara Schesser ; Ding, Yi ; Fujimoto, L. Miya ; Ring, Joshua G. ; Jain, Vishal ; Ram, Sanjay ; Marassi, Francesca M. ; Plano, Gregory V. / Yersinia pestis uses the Ail outer membrane protein to recruit vitronectin. In: Microbiology (United Kingdom). 2015 ; Vol. 161, No. 11. pp. 2174-2183.
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