XSET1 and p300 act synergistically, through coupled histone modifications, in transcriptional activation by p53

Zhanyun Tang, Wei Yi Chen, Miho Shimada, Uyen T T Nguyen, Jaehoon Kim, Xiao Jian Sun, Toru Sengoku, Robert K. McGinty, Joseph P. Fernandez, Tom W. Muir, Robert G. Roeder

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

The H3K4me3 mark in chromatin is closely correlated with actively transcribed genes, although the mechanisms involved in its generation and function are not fully understood. In vitro studies with recombinant chromatin and purified human factors demonstrate a robust SET1 complex (SET1C)-mediated H3K4 trimethylation that is dependent upon p53- and p300-mediated H3 acetylation, a corresponding SET1C-mediated enhancement of p53- and p300-dependent transcription that reflects a primary effect of SET1C through H3K4 trimethylation, and direct SET1C-p53 and SET1C-p300 interactions indicative of a targeted recruitment mechanism. Complementary cell-based assays demonstrate a DNA-damage-induced p53-SET1C interaction, a corresponding enrichment of SET1C and H3K4me3 on a p53 target gene (p21/WAF1), and a corresponding codependency of H3K4 trimethylation and transcription upon p300 and SET1C. These results establish a mechanism in which SET1C and p300 act cooperatively, through direct interactions and coupled histone modifications, to facilitate the function of p53.

Original languageEnglish (US)
JournalCell
Volume154
Issue number2
DOIs
StatePublished - Jul 18 2013
Externally publishedYes

Fingerprint

Histone Code
Transcription
Histones
Transcriptional Activation
Chromatin
Genes
Chemical activation
Acetylation
p53 Genes
Human engineering
DNA Damage
Assays
DNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tang, Z., Chen, W. Y., Shimada, M., Nguyen, U. T. T., Kim, J., Sun, X. J., ... Roeder, R. G. (2013). XSET1 and p300 act synergistically, through coupled histone modifications, in transcriptional activation by p53. Cell, 154(2). https://doi.org/10.1016/j.cell.2013.06.027

XSET1 and p300 act synergistically, through coupled histone modifications, in transcriptional activation by p53. / Tang, Zhanyun; Chen, Wei Yi; Shimada, Miho; Nguyen, Uyen T T; Kim, Jaehoon; Sun, Xiao Jian; Sengoku, Toru; McGinty, Robert K.; Fernandez, Joseph P.; Muir, Tom W.; Roeder, Robert G.

In: Cell, Vol. 154, No. 2, 18.07.2013.

Research output: Contribution to journalArticle

Tang, Z, Chen, WY, Shimada, M, Nguyen, UTT, Kim, J, Sun, XJ, Sengoku, T, McGinty, RK, Fernandez, JP, Muir, TW & Roeder, RG 2013, 'XSET1 and p300 act synergistically, through coupled histone modifications, in transcriptional activation by p53', Cell, vol. 154, no. 2. https://doi.org/10.1016/j.cell.2013.06.027
Tang, Zhanyun ; Chen, Wei Yi ; Shimada, Miho ; Nguyen, Uyen T T ; Kim, Jaehoon ; Sun, Xiao Jian ; Sengoku, Toru ; McGinty, Robert K. ; Fernandez, Joseph P. ; Muir, Tom W. ; Roeder, Robert G. / XSET1 and p300 act synergistically, through coupled histone modifications, in transcriptional activation by p53. In: Cell. 2013 ; Vol. 154, No. 2.
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