Xenotransplantation of porcine neonatal islets of Langerhans and Sertoli cells: A 4-year study

Rafael A. Valdés-González, Luis M. Dorantes, G. Nayely Garibay, Eduardo Bracho-Blanchet, Armando J. Mendez, Roberto Dávila-Pérez, Robert B. Elliott, Luis Terán, David J.G. White

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149 Scopus citations

Abstract

Objective: Porcine islets of Langerhans for xenotransplantation into humans have been proposed as a solution to the shortage of human donors. Rejection is one of the main constraints. This study presents the results of a clinical trial using a novel method for transplanting and immunoprotecting porcine islets in type 1 diabetic patients. Design: A 4-year follow up of a clinical trial involving 12 patients, with no immunosuppressive drugs at any point. Eleven age matched untransplanted diabetics served as controls. Methods: We have developed a procedure for protecting neonatal porcine islets by combining them with Sertoli cells and placing them in a novel subcutaneous autologous collagen-covered device. Results: In the patients in the treatment group, no complications arose and no porcine endogenous retrovirus infection was detected. Half of the patients showed a significant reduction in insulin requirements compared with both their pre transplant levels and controls, and this reduction was maintained for up to 4 years. Two patients became insulin-independent for several months. Porcine insulin was detected in three patients' sera following glucose stimulation up to 4 years post transplant. Three years post transplant, one of four devices was removed from four patients, and the presence of insulin-positive cells in the transplant was demonstrated by immunohistology in all 4 patients. Conclusions: Long-term cell survival with concurrent positive effects on metabolic control are possible by this technique.

Original languageEnglish (US)
Pages (from-to)419-427
Number of pages9
JournalEuropean Journal of Endocrinology
Volume153
Issue number3
DOIs
StatePublished - Sep 2005

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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    Valdés-González, R. A., Dorantes, L. M., Garibay, G. N., Bracho-Blanchet, E., Mendez, A. J., Dávila-Pérez, R., Elliott, R. B., Terán, L., & White, D. J. G. (2005). Xenotransplantation of porcine neonatal islets of Langerhans and Sertoli cells: A 4-year study. European Journal of Endocrinology, 153(3), 419-427. https://doi.org/10.1530/eje.1.01982