Xenotransplantation of porcine neonatal islets of Langerhans and Sertoli cells: A 4-year study

Rafael A. Valdés-González, Luis M. Dorantes, G. Nayely Garibay, Eduardo Bracho-Blanchet, Armando J Mendez, Roberto Dávila-Pérez, Robert B. Elliott, Luis Terán, David J G White

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

Objective: Porcine islets of Langerhans for xenotransplantation into humans have been proposed as a solution to the shortage of human donors. Rejection is one of the main constraints. This study presents the results of a clinical trial using a novel method for transplanting and immunoprotecting porcine islets in type 1 diabetic patients. Design: A 4-year follow up of a clinical trial involving 12 patients, with no immunosuppressive drugs at any point. Eleven age matched untransplanted diabetics served as controls. Methods: We have developed a procedure for protecting neonatal porcine islets by combining them with Sertoli cells and placing them in a novel subcutaneous autologous collagen-covered device. Results: In the patients in the treatment group, no complications arose and no porcine endogenous retrovirus infection was detected. Half of the patients showed a significant reduction in insulin requirements compared with both their pre transplant levels and controls, and this reduction was maintained for up to 4 years. Two patients became insulin-independent for several months. Porcine insulin was detected in three patients' sera following glucose stimulation up to 4 years post transplant. Three years post transplant, one of four devices was removed from four patients, and the presence of insulin-positive cells in the transplant was demonstrated by immunohistology in all 4 patients. Conclusions: Long-term cell survival with concurrent positive effects on metabolic control are possible by this technique.

Original languageEnglish
Pages (from-to)419-427
Number of pages9
JournalEuropean Journal of Endocrinology
Volume153
Issue number3
DOIs
StatePublished - Sep 1 2005

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Heterologous Transplantation
Langerhans Cells
Sertoli Cells
Islets of Langerhans
Swine
Transplants
Insulin
Retroviridae Infections
Insulin, Regular, Pork
Clinical Trials
Endogenous Retroviruses
Equipment and Supplies
Immunosuppressive Agents
Cell Survival
Collagen
Tissue Donors
Glucose
Serum

ASJC Scopus subject areas

  • Endocrinology

Cite this

Valdés-González, R. A., Dorantes, L. M., Garibay, G. N., Bracho-Blanchet, E., Mendez, A. J., Dávila-Pérez, R., ... White, D. J. G. (2005). Xenotransplantation of porcine neonatal islets of Langerhans and Sertoli cells: A 4-year study. European Journal of Endocrinology, 153(3), 419-427. https://doi.org/10.1530/eje.1.01982

Xenotransplantation of porcine neonatal islets of Langerhans and Sertoli cells : A 4-year study. / Valdés-González, Rafael A.; Dorantes, Luis M.; Garibay, G. Nayely; Bracho-Blanchet, Eduardo; Mendez, Armando J; Dávila-Pérez, Roberto; Elliott, Robert B.; Terán, Luis; White, David J G.

In: European Journal of Endocrinology, Vol. 153, No. 3, 01.09.2005, p. 419-427.

Research output: Contribution to journalArticle

Valdés-González, RA, Dorantes, LM, Garibay, GN, Bracho-Blanchet, E, Mendez, AJ, Dávila-Pérez, R, Elliott, RB, Terán, L & White, DJG 2005, 'Xenotransplantation of porcine neonatal islets of Langerhans and Sertoli cells: A 4-year study', European Journal of Endocrinology, vol. 153, no. 3, pp. 419-427. https://doi.org/10.1530/eje.1.01982
Valdés-González RA, Dorantes LM, Garibay GN, Bracho-Blanchet E, Mendez AJ, Dávila-Pérez R et al. Xenotransplantation of porcine neonatal islets of Langerhans and Sertoli cells: A 4-year study. European Journal of Endocrinology. 2005 Sep 1;153(3):419-427. https://doi.org/10.1530/eje.1.01982
Valdés-González, Rafael A. ; Dorantes, Luis M. ; Garibay, G. Nayely ; Bracho-Blanchet, Eduardo ; Mendez, Armando J ; Dávila-Pérez, Roberto ; Elliott, Robert B. ; Terán, Luis ; White, David J G. / Xenotransplantation of porcine neonatal islets of Langerhans and Sertoli cells : A 4-year study. In: European Journal of Endocrinology. 2005 ; Vol. 153, No. 3. pp. 419-427.
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