Abstract
The effect of stimulation of insulin secretion in pancreatic β cells on the elemental composition of these cells was investigated by x-ray microanalysis. In vitro experiments on isolated islets of Langerhans from ob/ob mice were compared to in situ experiments. The only significant difference in the elemental composition of β cells from ob/ob mice versus their lean counterparts is a lower Ca concentration in the ob/ob animals. The nucleus of the β cells has a higher concentration of P, K, and Na than the cytoplasm, which has a higher concentration of S and Cl. No polarized ion distribution in the cytoplasm of the β cells was observed. Isolated β cells show a higher concentration of Na and Cl and a lower concentration of K than their in sire counterparts. Stimulation of insulin secretion with glucose both in situ and in vitro showed only very small effects on the elemental composition of the β cells: a tendency to a decreased P content was noted. In vitro experiments using stimulation with high extracellular K+ showed, in addition, a small increase in the intracellular K concentration. In conclusion, while the elemental content of β cells in vitro differs from that in situ, the response to glucose stimulation appears to be similar in both systems.
| Original language | English |
|---|---|
| Pages (from-to) | 134-140 |
| Number of pages | 7 |
| Journal | Pancreas |
| Volume | 16 |
| Issue number | 2 |
| State | Published - Mar 1 1998 |
| Externally published | Yes |
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Keywords
- Barium
- Calcium
- Depolarization
- Endocrine pancreas
- Glucose stimulation
- X- ray microanalysis
ASJC Scopus subject areas
- Gastroenterology
- Endocrinology
Cite this
X-ray microanalysis of in situ and isolated pancreatic islets. / Wróblewski, Romuald; Pålsgård, Eva; Juntti-Berggren, Lisa; Berggren, Per Olof; Roomans, Godfried M.
In: Pancreas, Vol. 16, No. 2, 01.03.1998, p. 134-140.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - X-ray microanalysis of in situ and isolated pancreatic islets
AU - Wróblewski, Romuald
AU - Pålsgård, Eva
AU - Juntti-Berggren, Lisa
AU - Berggren, Per Olof
AU - Roomans, Godfried M.
PY - 1998/3/1
Y1 - 1998/3/1
N2 - The effect of stimulation of insulin secretion in pancreatic β cells on the elemental composition of these cells was investigated by x-ray microanalysis. In vitro experiments on isolated islets of Langerhans from ob/ob mice were compared to in situ experiments. The only significant difference in the elemental composition of β cells from ob/ob mice versus their lean counterparts is a lower Ca concentration in the ob/ob animals. The nucleus of the β cells has a higher concentration of P, K, and Na than the cytoplasm, which has a higher concentration of S and Cl. No polarized ion distribution in the cytoplasm of the β cells was observed. Isolated β cells show a higher concentration of Na and Cl and a lower concentration of K than their in sire counterparts. Stimulation of insulin secretion with glucose both in situ and in vitro showed only very small effects on the elemental composition of the β cells: a tendency to a decreased P content was noted. In vitro experiments using stimulation with high extracellular K+ showed, in addition, a small increase in the intracellular K concentration. In conclusion, while the elemental content of β cells in vitro differs from that in situ, the response to glucose stimulation appears to be similar in both systems.
AB - The effect of stimulation of insulin secretion in pancreatic β cells on the elemental composition of these cells was investigated by x-ray microanalysis. In vitro experiments on isolated islets of Langerhans from ob/ob mice were compared to in situ experiments. The only significant difference in the elemental composition of β cells from ob/ob mice versus their lean counterparts is a lower Ca concentration in the ob/ob animals. The nucleus of the β cells has a higher concentration of P, K, and Na than the cytoplasm, which has a higher concentration of S and Cl. No polarized ion distribution in the cytoplasm of the β cells was observed. Isolated β cells show a higher concentration of Na and Cl and a lower concentration of K than their in sire counterparts. Stimulation of insulin secretion with glucose both in situ and in vitro showed only very small effects on the elemental composition of the β cells: a tendency to a decreased P content was noted. In vitro experiments using stimulation with high extracellular K+ showed, in addition, a small increase in the intracellular K concentration. In conclusion, while the elemental content of β cells in vitro differs from that in situ, the response to glucose stimulation appears to be similar in both systems.
KW - Barium
KW - Calcium
KW - Depolarization
KW - Endocrine pancreas
KW - Glucose stimulation
KW - X- ray microanalysis
UR - http://www.scopus.com/inward/record.url?scp=0038428594&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038428594&partnerID=8YFLogxK
M3 - Article
C2 - 9510135
AN - SCOPUS:0038428594
VL - 16
SP - 134
EP - 140
JO - Pancreas
JF - Pancreas
SN - 0885-3177
IS - 2
ER -