TY - JOUR
T1 - X-ray microanalysis of in situ and isolated pancreatic islets
AU - Wróblewski, Romuald
AU - Pålsgård, Eva
AU - Juntti-Berggren, Lisa
AU - Berggren, Per Olof
AU - Roomans, Godfried M.
PY - 1998/3
Y1 - 1998/3
N2 - The effect of stimulation of insulin secretion in pancreatic β cells on the elemental composition of these cells was investigated by x-ray microanalysis. In vitro experiments on isolated islets of Langerhans from ob/ob mice were compared to in situ experiments. The only significant difference in the elemental composition of β cells from ob/ob mice versus their lean counterparts is a lower Ca concentration in the ob/ob animals. The nucleus of the β cells has a higher concentration of P, K, and Na than the cytoplasm, which has a higher concentration of S and Cl. No polarized ion distribution in the cytoplasm of the β cells was observed. Isolated β cells show a higher concentration of Na and Cl and a lower concentration of K than their in sire counterparts. Stimulation of insulin secretion with glucose both in situ and in vitro showed only very small effects on the elemental composition of the β cells: a tendency to a decreased P content was noted. In vitro experiments using stimulation with high extracellular K+ showed, in addition, a small increase in the intracellular K concentration. In conclusion, while the elemental content of β cells in vitro differs from that in situ, the response to glucose stimulation appears to be similar in both systems.
AB - The effect of stimulation of insulin secretion in pancreatic β cells on the elemental composition of these cells was investigated by x-ray microanalysis. In vitro experiments on isolated islets of Langerhans from ob/ob mice were compared to in situ experiments. The only significant difference in the elemental composition of β cells from ob/ob mice versus their lean counterparts is a lower Ca concentration in the ob/ob animals. The nucleus of the β cells has a higher concentration of P, K, and Na than the cytoplasm, which has a higher concentration of S and Cl. No polarized ion distribution in the cytoplasm of the β cells was observed. Isolated β cells show a higher concentration of Na and Cl and a lower concentration of K than their in sire counterparts. Stimulation of insulin secretion with glucose both in situ and in vitro showed only very small effects on the elemental composition of the β cells: a tendency to a decreased P content was noted. In vitro experiments using stimulation with high extracellular K+ showed, in addition, a small increase in the intracellular K concentration. In conclusion, while the elemental content of β cells in vitro differs from that in situ, the response to glucose stimulation appears to be similar in both systems.
KW - Barium
KW - Calcium
KW - Depolarization
KW - Endocrine pancreas
KW - Glucose stimulation
KW - X- ray microanalysis
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U2 - 10.1097/00006676-199803000-00005
DO - 10.1097/00006676-199803000-00005
M3 - Article
C2 - 9510135
AN - SCOPUS:0038428594
VL - 16
SP - 134
EP - 140
JO - Pancreas
JF - Pancreas
SN - 0885-3177
IS - 2
ER -