X-ray microanalysis of in situ and isolated pancreatic islets

Romuald Wróblewski, Eva Pålsgård, Lisa Juntti-Berggren, Per Olof Berggren, Godfried M. Roomans

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The effect of stimulation of insulin secretion in pancreatic β cells on the elemental composition of these cells was investigated by x-ray microanalysis. In vitro experiments on isolated islets of Langerhans from ob/ob mice were compared to in situ experiments. The only significant difference in the elemental composition of β cells from ob/ob mice versus their lean counterparts is a lower Ca concentration in the ob/ob animals. The nucleus of the β cells has a higher concentration of P, K, and Na than the cytoplasm, which has a higher concentration of S and Cl. No polarized ion distribution in the cytoplasm of the β cells was observed. Isolated β cells show a higher concentration of Na and Cl and a lower concentration of K than their in sire counterparts. Stimulation of insulin secretion with glucose both in situ and in vitro showed only very small effects on the elemental composition of the β cells: a tendency to a decreased P content was noted. In vitro experiments using stimulation with high extracellular K+ showed, in addition, a small increase in the intracellular K concentration. In conclusion, while the elemental content of β cells in vitro differs from that in situ, the response to glucose stimulation appears to be similar in both systems.

Original languageEnglish (US)
Pages (from-to)134-140
Number of pages7
JournalPancreas
Volume16
Issue number2
DOIs
StatePublished - Mar 1998

Keywords

  • Barium
  • Calcium
  • Depolarization
  • Endocrine pancreas
  • Glucose stimulation
  • X- ray microanalysis

ASJC Scopus subject areas

  • Gastroenterology
  • Endocrinology

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    Wróblewski, R., Pålsgård, E., Juntti-Berggren, L., Berggren, P. O., & Roomans, G. M. (1998). X-ray microanalysis of in situ and isolated pancreatic islets. Pancreas, 16(2), 134-140. https://doi.org/10.1097/00006676-199803000-00005