X-ray microanalysis of in situ and isolated pancreatic islets

Romuald Wróblewski, Eva Pålsgård, Lisa Juntti-Berggren, Per Olof Berggren, Godfried M. Roomans

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of stimulation of insulin secretion in pancreatic β cells on the elemental composition of these cells was investigated by x-ray microanalysis. In vitro experiments on isolated islets of Langerhans from ob/ob mice were compared to in situ experiments. The only significant difference in the elemental composition of β cells from ob/ob mice versus their lean counterparts is a lower Ca concentration in the ob/ob animals. The nucleus of the β cells has a higher concentration of P, K, and Na than the cytoplasm, which has a higher concentration of S and Cl. No polarized ion distribution in the cytoplasm of the β cells was observed. Isolated β cells show a higher concentration of Na and Cl and a lower concentration of K than their in sire counterparts. Stimulation of insulin secretion with glucose both in situ and in vitro showed only very small effects on the elemental composition of the β cells: a tendency to a decreased P content was noted. In vitro experiments using stimulation with high extracellular K+ showed, in addition, a small increase in the intracellular K concentration. In conclusion, while the elemental content of β cells in vitro differs from that in situ, the response to glucose stimulation appears to be similar in both systems.

Original languageEnglish
Pages (from-to)134-140
Number of pages7
JournalPancreas
Volume16
Issue number2
StatePublished - Mar 1 1998
Externally publishedYes

Fingerprint

Electron Probe Microanalysis
Islets of Langerhans
Cytoplasm
Insulin
Glucose
Cell Nucleus
X-Rays
Ions
In Vitro Techniques

Keywords

  • Barium
  • Calcium
  • Depolarization
  • Endocrine pancreas
  • Glucose stimulation
  • X- ray microanalysis

ASJC Scopus subject areas

  • Gastroenterology
  • Endocrinology

Cite this

Wróblewski, R., Pålsgård, E., Juntti-Berggren, L., Berggren, P. O., & Roomans, G. M. (1998). X-ray microanalysis of in situ and isolated pancreatic islets. Pancreas, 16(2), 134-140.

X-ray microanalysis of in situ and isolated pancreatic islets. / Wróblewski, Romuald; Pålsgård, Eva; Juntti-Berggren, Lisa; Berggren, Per Olof; Roomans, Godfried M.

In: Pancreas, Vol. 16, No. 2, 01.03.1998, p. 134-140.

Research output: Contribution to journalArticle

Wróblewski, R, Pålsgård, E, Juntti-Berggren, L, Berggren, PO & Roomans, GM 1998, 'X-ray microanalysis of in situ and isolated pancreatic islets', Pancreas, vol. 16, no. 2, pp. 134-140.
Wróblewski R, Pålsgård E, Juntti-Berggren L, Berggren PO, Roomans GM. X-ray microanalysis of in situ and isolated pancreatic islets. Pancreas. 1998 Mar 1;16(2):134-140.
Wróblewski, Romuald ; Pålsgård, Eva ; Juntti-Berggren, Lisa ; Berggren, Per Olof ; Roomans, Godfried M. / X-ray microanalysis of in situ and isolated pancreatic islets. In: Pancreas. 1998 ; Vol. 16, No. 2. pp. 134-140.
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