Wound Healing Assay for Melanoma Cell Migration

Juliano T. Freitas, Ivan Jozic, Barbara Bedogni

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations


Cell migration is a critical process involved in morphogenesis, inflammation, and cancer metastasis. Wound healing assay is a simple, non-expensive, and highly reproducible method to study cancer cell migration in vitro. It is based on the observation that cells growing in a monolayer migrate to re-establish cell contacts after the development of an artificial wound. The assay involves creation of a wound in a monolayer, image acquisition during wound closure, and comparison of migrated area at initial and final time points.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages7
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Cell migration
  • Melanoma
  • Metastasis
  • Wound healing
  • Wound scratch

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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