Wound Healing Assay for Melanoma Cell Migration

Juliano T. Freitas, Ivan Jozic, Barbara Bedogni

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cell migration is a critical process involved in morphogenesis, inflammation, and cancer metastasis. Wound healing assay is a simple, non-expensive, and highly reproducible method to study cancer cell migration in vitro. It is based on the observation that cells growing in a monolayer migrate to re-establish cell contacts after the development of an artificial wound. The assay involves creation of a wound in a monolayer, image acquisition during wound closure, and comparison of migrated area at initial and final time points.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages65-71
Number of pages7
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2265
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell migration
  • Melanoma
  • Metastasis
  • Wound healing
  • Wound scratch

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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