Abstract
Crude oil-derived polycyclic aromatic hydrocarbons (PAHs) are pervasive environmental pollutants with well-established detrimental effects on the health of marine organisms. Following large-scale oil spills in the marine environment, there is a critical need for noninvasive sampling methods to assess environmental exposure to PAHs without further perturbations to the population and for long-term monitoring following a spill. To test the efficacy of epidermal mucus mRNA as a source for noninvasive sampling, juvenile mahi-mahi (Coryphaena hippurus, 28 days of age) were exposed to control seawater or two concentrations of high-energy water accommodated fractions (HEWAFs; 5% or 10%) of Deepwater Horizon surface oil for 48 h. Whole-transcriptome sequencing revealed differential expression of 501 transcripts in the low-HEWAF exposure (PAH = 16.55 μg/L) and 196 transcripts in the high-HEWAF exposure (PAH = 23.03 μg/L), suggesting differential regulation of mRNA in mucus following PAH exposure. In addition to differential expression of well-established biomarkers of PAH exposure such as cytochrome P450 enzymes, the mucosal transcriptome showed differential expression of transcripts involved in immune response, cardiotoxicity, and calcium homeostasis that parallel molecular responses in whole embryos. The consistency of the changes in expression in the epidermal mucus compared to that of tissues obtained from lethal sampling suggests that mucus is a promising source for noninvasive monitoring techniques.
| Original language | English (US) |
|---|---|
| Journal | Environmental Science and Technology Letters |
| DOIs | |
| State | Accepted/In press - Jan 1 2019 |
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ASJC Scopus subject areas
- Environmental Chemistry
- Ecology
- Water Science and Technology
- Waste Management and Disposal
- Pollution
- Health, Toxicology and Mutagenesis
Cite this
Whole-Transcriptome Sequencing of Epidermal Mucus as a Novel Method for Oil Exposure Assessment in Juvenile Mahi-Mahi (Coryphaena hippurus). / Greer, Justin B.; Andrzejczyk, Nicolette E.; Mager, Edward M.; Stieglitz, John D.; Benetti, Daniel; Grosell, Martin; Schlenk, Daniel.
In: Environmental Science and Technology Letters, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Whole-Transcriptome Sequencing of Epidermal Mucus as a Novel Method for Oil Exposure Assessment in Juvenile Mahi-Mahi (Coryphaena hippurus)
AU - Greer, Justin B.
AU - Andrzejczyk, Nicolette E.
AU - Mager, Edward M.
AU - Stieglitz, John D.
AU - Benetti, Daniel
AU - Grosell, Martin
AU - Schlenk, Daniel
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Crude oil-derived polycyclic aromatic hydrocarbons (PAHs) are pervasive environmental pollutants with well-established detrimental effects on the health of marine organisms. Following large-scale oil spills in the marine environment, there is a critical need for noninvasive sampling methods to assess environmental exposure to PAHs without further perturbations to the population and for long-term monitoring following a spill. To test the efficacy of epidermal mucus mRNA as a source for noninvasive sampling, juvenile mahi-mahi (Coryphaena hippurus, 28 days of age) were exposed to control seawater or two concentrations of high-energy water accommodated fractions (HEWAFs; 5% or 10%) of Deepwater Horizon surface oil for 48 h. Whole-transcriptome sequencing revealed differential expression of 501 transcripts in the low-HEWAF exposure (PAH = 16.55 μg/L) and 196 transcripts in the high-HEWAF exposure (PAH = 23.03 μg/L), suggesting differential regulation of mRNA in mucus following PAH exposure. In addition to differential expression of well-established biomarkers of PAH exposure such as cytochrome P450 enzymes, the mucosal transcriptome showed differential expression of transcripts involved in immune response, cardiotoxicity, and calcium homeostasis that parallel molecular responses in whole embryos. The consistency of the changes in expression in the epidermal mucus compared to that of tissues obtained from lethal sampling suggests that mucus is a promising source for noninvasive monitoring techniques.
AB - Crude oil-derived polycyclic aromatic hydrocarbons (PAHs) are pervasive environmental pollutants with well-established detrimental effects on the health of marine organisms. Following large-scale oil spills in the marine environment, there is a critical need for noninvasive sampling methods to assess environmental exposure to PAHs without further perturbations to the population and for long-term monitoring following a spill. To test the efficacy of epidermal mucus mRNA as a source for noninvasive sampling, juvenile mahi-mahi (Coryphaena hippurus, 28 days of age) were exposed to control seawater or two concentrations of high-energy water accommodated fractions (HEWAFs; 5% or 10%) of Deepwater Horizon surface oil for 48 h. Whole-transcriptome sequencing revealed differential expression of 501 transcripts in the low-HEWAF exposure (PAH = 16.55 μg/L) and 196 transcripts in the high-HEWAF exposure (PAH = 23.03 μg/L), suggesting differential regulation of mRNA in mucus following PAH exposure. In addition to differential expression of well-established biomarkers of PAH exposure such as cytochrome P450 enzymes, the mucosal transcriptome showed differential expression of transcripts involved in immune response, cardiotoxicity, and calcium homeostasis that parallel molecular responses in whole embryos. The consistency of the changes in expression in the epidermal mucus compared to that of tissues obtained from lethal sampling suggests that mucus is a promising source for noninvasive monitoring techniques.
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UR - http://www.scopus.com/inward/citedby.url?scp=85072511443&partnerID=8YFLogxK
U2 - 10.1021/acs.estlett.9b00479
DO - 10.1021/acs.estlett.9b00479
M3 - Article
AN - SCOPUS:85072511443
JO - Environmental Science and Technology Letters
JF - Environmental Science and Technology Letters
SN - 2328-8930
ER -