Whole organ decellularization - A tool for bioscaffold fabrication and organ bioengineering

Pedro M. Baptista, Giuseppe Orlando, Sayed Hadi Mirmalek-Sani, Mohummad Siddiqui, Anthony Atala, Shay Soker

Research output: Chapter in Book/Report/Conference proceedingConference contribution

119 Citations (Scopus)

Abstract

The use of synthetic and naturally-derived scaffolds for bioengineering of solid organs has been limited due to a lack of an integrated vascular network. Here, we describe fabrication of a bioscaffold system with intact vascular tree. Animal-donor organs and tissues, ranging in size up-to thirty centimeters, were perfused with decellularization solution to selectively remove the cellular component of the tissue and leave an intact extracellular matrix and vascular network. The vascular tree demonstrated sequential fluid flow through a central inlet vessel that branched into an extensive capillary bed and coalesced back into a single outlet vessel. In one example, the liver, we used central inlet vessels to perfuse human and animal liver cells through the bioscaffold to create a functional liver tissue construct in vitro. These results demonstrate a novel yet simple and scalable method to obtain whole organ vascularized bioscaffolds with potential for liver, kidney, pancreas, intestine and other organs' bioengineering. These bioscaffolds can further provide a tool to study cells in their natural three-dimensional environment, which is superior for drug discovery platform compared with cells cultured in two-dimensional petri dishes.

Original languageEnglish
Title of host publicationProceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009
Pages6526-6529
Number of pages4
DOIs
StatePublished - Dec 1 2009
Externally publishedYes
Event31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009 - Minneapolis, MN, United States
Duration: Sep 2 2009Sep 6 2009

Other

Other31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009
CountryUnited States
CityMinneapolis, MN
Period9/2/099/6/09

Fingerprint

Bioengineering
Liver
Blood Vessels
Fabrication
Tissue
Animals
Animal Structures
Drug Discovery
Scaffolds
Intestines
Extracellular Matrix
Flow of fluids
Pancreas
Cultured Cells
Tissue Donors
Kidney

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Biomedical Engineering
  • Medicine(all)

Cite this

Baptista, P. M., Orlando, G., Mirmalek-Sani, S. H., Siddiqui, M., Atala, A., & Soker, S. (2009). Whole organ decellularization - A tool for bioscaffold fabrication and organ bioengineering. In Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009 (pp. 6526-6529). [5333145] https://doi.org/10.1109/IEMBS.2009.5333145

Whole organ decellularization - A tool for bioscaffold fabrication and organ bioengineering. / Baptista, Pedro M.; Orlando, Giuseppe; Mirmalek-Sani, Sayed Hadi; Siddiqui, Mohummad; Atala, Anthony; Soker, Shay.

Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009. 2009. p. 6526-6529 5333145.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Baptista, PM, Orlando, G, Mirmalek-Sani, SH, Siddiqui, M, Atala, A & Soker, S 2009, Whole organ decellularization - A tool for bioscaffold fabrication and organ bioengineering. in Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009., 5333145, pp. 6526-6529, 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009, Minneapolis, MN, United States, 9/2/09. https://doi.org/10.1109/IEMBS.2009.5333145
Baptista PM, Orlando G, Mirmalek-Sani SH, Siddiqui M, Atala A, Soker S. Whole organ decellularization - A tool for bioscaffold fabrication and organ bioengineering. In Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009. 2009. p. 6526-6529. 5333145 https://doi.org/10.1109/IEMBS.2009.5333145
Baptista, Pedro M. ; Orlando, Giuseppe ; Mirmalek-Sani, Sayed Hadi ; Siddiqui, Mohummad ; Atala, Anthony ; Soker, Shay. / Whole organ decellularization - A tool for bioscaffold fabrication and organ bioengineering. Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009. 2009. pp. 6526-6529
@inproceedings{0b18a85bd0be4810a60f7002c4f643be,
title = "Whole organ decellularization - A tool for bioscaffold fabrication and organ bioengineering",
abstract = "The use of synthetic and naturally-derived scaffolds for bioengineering of solid organs has been limited due to a lack of an integrated vascular network. Here, we describe fabrication of a bioscaffold system with intact vascular tree. Animal-donor organs and tissues, ranging in size up-to thirty centimeters, were perfused with decellularization solution to selectively remove the cellular component of the tissue and leave an intact extracellular matrix and vascular network. The vascular tree demonstrated sequential fluid flow through a central inlet vessel that branched into an extensive capillary bed and coalesced back into a single outlet vessel. In one example, the liver, we used central inlet vessels to perfuse human and animal liver cells through the bioscaffold to create a functional liver tissue construct in vitro. These results demonstrate a novel yet simple and scalable method to obtain whole organ vascularized bioscaffolds with potential for liver, kidney, pancreas, intestine and other organs' bioengineering. These bioscaffolds can further provide a tool to study cells in their natural three-dimensional environment, which is superior for drug discovery platform compared with cells cultured in two-dimensional petri dishes.",
author = "Baptista, {Pedro M.} and Giuseppe Orlando and Mirmalek-Sani, {Sayed Hadi} and Mohummad Siddiqui and Anthony Atala and Shay Soker",
year = "2009",
month = "12",
day = "1",
doi = "10.1109/IEMBS.2009.5333145",
language = "English",
isbn = "9781424432967",
pages = "6526--6529",
booktitle = "Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009",

}

TY - GEN

T1 - Whole organ decellularization - A tool for bioscaffold fabrication and organ bioengineering

AU - Baptista, Pedro M.

AU - Orlando, Giuseppe

AU - Mirmalek-Sani, Sayed Hadi

AU - Siddiqui, Mohummad

AU - Atala, Anthony

AU - Soker, Shay

PY - 2009/12/1

Y1 - 2009/12/1

N2 - The use of synthetic and naturally-derived scaffolds for bioengineering of solid organs has been limited due to a lack of an integrated vascular network. Here, we describe fabrication of a bioscaffold system with intact vascular tree. Animal-donor organs and tissues, ranging in size up-to thirty centimeters, were perfused with decellularization solution to selectively remove the cellular component of the tissue and leave an intact extracellular matrix and vascular network. The vascular tree demonstrated sequential fluid flow through a central inlet vessel that branched into an extensive capillary bed and coalesced back into a single outlet vessel. In one example, the liver, we used central inlet vessels to perfuse human and animal liver cells through the bioscaffold to create a functional liver tissue construct in vitro. These results demonstrate a novel yet simple and scalable method to obtain whole organ vascularized bioscaffolds with potential for liver, kidney, pancreas, intestine and other organs' bioengineering. These bioscaffolds can further provide a tool to study cells in their natural three-dimensional environment, which is superior for drug discovery platform compared with cells cultured in two-dimensional petri dishes.

AB - The use of synthetic and naturally-derived scaffolds for bioengineering of solid organs has been limited due to a lack of an integrated vascular network. Here, we describe fabrication of a bioscaffold system with intact vascular tree. Animal-donor organs and tissues, ranging in size up-to thirty centimeters, were perfused with decellularization solution to selectively remove the cellular component of the tissue and leave an intact extracellular matrix and vascular network. The vascular tree demonstrated sequential fluid flow through a central inlet vessel that branched into an extensive capillary bed and coalesced back into a single outlet vessel. In one example, the liver, we used central inlet vessels to perfuse human and animal liver cells through the bioscaffold to create a functional liver tissue construct in vitro. These results demonstrate a novel yet simple and scalable method to obtain whole organ vascularized bioscaffolds with potential for liver, kidney, pancreas, intestine and other organs' bioengineering. These bioscaffolds can further provide a tool to study cells in their natural three-dimensional environment, which is superior for drug discovery platform compared with cells cultured in two-dimensional petri dishes.

UR - http://www.scopus.com/inward/record.url?scp=77950978556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950978556&partnerID=8YFLogxK

U2 - 10.1109/IEMBS.2009.5333145

DO - 10.1109/IEMBS.2009.5333145

M3 - Conference contribution

SN - 9781424432967

SP - 6526

EP - 6529

BT - Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009

ER -