Whole-cell biosensors as tools for the detection of quorum-sensing molecules: Uses in diagnostics and the investigation of the quorum-sensing mechanism

Gregory O'Connor, Leslie D. Knecht, Nelson Salgado, Sebastian Strobel, Patrizia Pasini, Sylvia Daunert

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

Genetically engineered bacterial whole-cell biosensors are powerful tools that take advantage of bacterial proteins and pathways to allow for detection of a specific analyte. These biosensors have been employed for a broad range of applications, including the detection of bacterial quorum-sensing molecules (QSMs). Bacterial QSMs are the small molecules bacteria use for population density-dependent communication, a process referred to as quorum sensing (QS). Various research groups have investigated the presence of QSMs, including N-acyl homoserine lactones (AHLs) and autoinducer-2 (AI-2), in physiological samples in attempts to enhance our knowledge of the role of bacteria and QS in disease states. Continued studies in these fields may allow for improved patient care and therapeutics based upon QSMs. Furthermore, bacterial whole-cell biosensors have elucidated the roles of some antibiotics as QS agonists and antagonists.

Original languageEnglish (US)
Title of host publicationAdvances in Biochemical Engineering/Biotechnology
PublisherSpringer Science and Business Media Deutschland GmbH
Pages181-200
Number of pages20
Volume154
DOIs
StatePublished - 2016

Publication series

NameAdvances in Biochemical Engineering/Biotechnology
Volume154
ISSN (Print)07246145

Keywords

  • Autoinducer-2 (AI-2)
  • Cystic Fibrosis (CF)
  • Inflammatory Bowel Disease (IBD)
  • N-acyl homoserine lactones (AHLs)
  • Quorum-sensing (QS)
  • Quorum-sensing molecules (QSMs)
  • Urinary Tract Infections (UTI)
  • Whole-cell biosensors

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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  • Cite this

    O'Connor, G., Knecht, L. D., Salgado, N., Strobel, S., Pasini, P., & Daunert, S. (2016). Whole-cell biosensors as tools for the detection of quorum-sensing molecules: Uses in diagnostics and the investigation of the quorum-sensing mechanism. In Advances in Biochemical Engineering/Biotechnology (Vol. 154, pp. 181-200). (Advances in Biochemical Engineering/Biotechnology; Vol. 154). Springer Science and Business Media Deutschland GmbH. https://doi.org/10.1007/10_2015_337