Whole-body periodic acceleration modifies experimental asthma in sheep

William M. Abraham, Ashfaq Ahmed, Irakli Serebriakov, Isabel T. Lauredo, Jorge Bassuk, Jose A. Adams, Marvin A. Sackner

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Rationale: Nitric oxide is released from vascular endothelium in response to increased pulsatile shear stress. Nitric oxide inhibits mast cell activation and is antiinflammatory and therefore might be protective in asthma. Objectives: We determined if a noninvasive motion platform that imparts periodic sinusoidal inertial forces to the whole body along the spinal axis (pGz) causing release of endothelial nitric oxide modulates experimental asthma in sheep. Methods: Allergic sheep were untreated (control) or were treated with pGz alone or after receiving intravenously the nitric oxide synthase inhibitor N w-nitro-L-arginine methyl ester (L-NAME) before aerosol challenge with Ascaris suum, and the effect on antigen-induced airway responses was determined. Bronchoalveolar lavage cells obtained 6 h after antigen challenge were analyzed for nuclear factor-κB (NF-κB) activity in the respective groups. Results: pGz treatment for 1 h before antigen challenge reduced the early airway response and blocked the late airway response but did not prevent the antigen-induced airway hyperresponsiveness 24 h after challenge. Administration of L-NAME before pGz completely reversed this protection, whereas L-NAME alone did not affect the antigen-induced responses. NF-κB activity was 1.9- and 1.8-fold higher in the control and L-NAME + pGz groups, respectively, compared with pGz-treated animals. Extending the pGz treatment to twice daily for 3 d and then 1 h before antigen challenge blocked the early and late airway responses, the 24-h airway hyperresponsiveness, and the airway inflammatory cell response. Conclusion: Whole-body pGz modulates allergen-induced airway responses in allergic sheep.

Original languageEnglish (US)
Pages (from-to)743-752
Number of pages10
JournalAmerican journal of respiratory and critical care medicine
Volume174
Issue number7
DOIs
StatePublished - Oct 1 2006

Keywords

  • Animal models
  • Asthma therapy
  • Nitric oxide
  • Noninvasive periodic acceleration

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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    Abraham, W. M., Ahmed, A., Serebriakov, I., Lauredo, I. T., Bassuk, J., Adams, J. A., & Sackner, M. A. (2006). Whole-body periodic acceleration modifies experimental asthma in sheep. American journal of respiratory and critical care medicine, 174(7), 743-752. https://doi.org/10.1164/rccm.200601-048OC