What increases type III procollagen mRNA levels in lung tissue: Stress induced by changes in force or amplitude?

Cristiane S.N.B. Garcia, Patricia R.M. Rocco, Lívia D. Facchinetti, Roberta M. Lassance, Pedro Caruso, Daniel Deheinzelin, Marcelo M. Morales, Pablo V. Romero, Débora S. Faffe, Walter A. Zin

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

We hypothesized that stress determined by force could induce higher type III procollagen (PCIII) mRNA expression than the stress determined by amplitude. To that end, rat lung tissue strips were oscillated for 1 h under different amplitudes [1, 5 and 10% of resting length (L B), at 0.5 × 10 -2 N] and forces (0.25 × 10 -2, 0.5 × 10 -2 and 10 -2 N, at 5% L B). Resistance (R), elastance (E) and hysteresivity (η) were analysed during sinusoidal oscillations at 1 Hz. After 1 h of oscillation, PCIII mRNA expression was determined by Northern-blot and semiquantitative RT-PCR. Control value of PCIII mRNA was obtained from unstressed strips. E and R increased with augmenting force and decreased with increasing amplitude, while η remained unaltered. PCIII mRNA expression increased significantly after 1 h of oscillation at 10 -2 N and 5% L B and remained unchanged for 6 h. In conclusion, the stress induced by force but not by amplitude led to the increment in PCIII mRNA expression.

Original languageEnglish (US)
Pages (from-to)59-70
Number of pages12
JournalRespiratory Physiology and Neurobiology
Volume144
Issue number1
DOIs
StatePublished - Nov 30 2004
Externally publishedYes

Keywords

  • Extracellular matrix
  • Hysteresivity
  • Mechanical forces
  • Procollagen mRNA
  • Rat
  • Tissue mechanics

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology
  • Pulmonary and Respiratory Medicine

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    Garcia, C. S. N. B., Rocco, P. R. M., Facchinetti, L. D., Lassance, R. M., Caruso, P., Deheinzelin, D., Morales, M. M., Romero, P. V., Faffe, D. S., & Zin, W. A. (2004). What increases type III procollagen mRNA levels in lung tissue: Stress induced by changes in force or amplitude? Respiratory Physiology and Neurobiology, 144(1), 59-70. https://doi.org/10.1016/j.resp.2004.07.023