TY - JOUR
T1 - What increases type III procollagen mRNA levels in lung tissue
T2 - Stress induced by changes in force or amplitude?
AU - Garcia, Cristiane S.N.B.
AU - Rocco, Patricia R.M.
AU - Facchinetti, Lívia D.
AU - Lassance, Roberta M.
AU - Caruso, Pedro
AU - Deheinzelin, Daniel
AU - Morales, Marcelo M.
AU - Romero, Pablo V.
AU - Faffe, Débora S.
AU - Zin, Walter A.
PY - 2004/11/30
Y1 - 2004/11/30
N2 - We hypothesized that stress determined by force could induce higher type III procollagen (PCIII) mRNA expression than the stress determined by amplitude. To that end, rat lung tissue strips were oscillated for 1 h under different amplitudes [1, 5 and 10% of resting length (L B), at 0.5 × 10 -2 N] and forces (0.25 × 10 -2, 0.5 × 10 -2 and 10 -2 N, at 5% L B). Resistance (R), elastance (E) and hysteresivity (η) were analysed during sinusoidal oscillations at 1 Hz. After 1 h of oscillation, PCIII mRNA expression was determined by Northern-blot and semiquantitative RT-PCR. Control value of PCIII mRNA was obtained from unstressed strips. E and R increased with augmenting force and decreased with increasing amplitude, while η remained unaltered. PCIII mRNA expression increased significantly after 1 h of oscillation at 10 -2 N and 5% L B and remained unchanged for 6 h. In conclusion, the stress induced by force but not by amplitude led to the increment in PCIII mRNA expression.
AB - We hypothesized that stress determined by force could induce higher type III procollagen (PCIII) mRNA expression than the stress determined by amplitude. To that end, rat lung tissue strips were oscillated for 1 h under different amplitudes [1, 5 and 10% of resting length (L B), at 0.5 × 10 -2 N] and forces (0.25 × 10 -2, 0.5 × 10 -2 and 10 -2 N, at 5% L B). Resistance (R), elastance (E) and hysteresivity (η) were analysed during sinusoidal oscillations at 1 Hz. After 1 h of oscillation, PCIII mRNA expression was determined by Northern-blot and semiquantitative RT-PCR. Control value of PCIII mRNA was obtained from unstressed strips. E and R increased with augmenting force and decreased with increasing amplitude, while η remained unaltered. PCIII mRNA expression increased significantly after 1 h of oscillation at 10 -2 N and 5% L B and remained unchanged for 6 h. In conclusion, the stress induced by force but not by amplitude led to the increment in PCIII mRNA expression.
KW - Extracellular matrix
KW - Hysteresivity
KW - Mechanical forces
KW - Procollagen mRNA
KW - Rat
KW - Tissue mechanics
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U2 - 10.1016/j.resp.2004.07.023
DO - 10.1016/j.resp.2004.07.023
M3 - Article
C2 - 15522703
AN - SCOPUS:7544239764
VL - 144
SP - 59
EP - 70
JO - Respiratory Physiology and Neurobiology
JF - Respiratory Physiology and Neurobiology
SN - 1569-9048
IS - 1
ER -