Waterborne copper exposure inhibits ammonia excretion and branchial carbonic anhydrase activity in euryhaline guppies acclimated to both fresh water and sea water

Alex M. Zimmer, Indianara Fernanda Barcarolli, Chris M. Wood, Adalto Bianchini

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Inhibition of ammonia excretion (J amm) is a common response to Cu exposure in freshwater (FW) and seawater (SW) organisms. To determine the mechanism of this response, a euryhaline species of guppy (Poecilia vivipara) was exposed to 20μg Cu/l in FW (0ppt) and SW (25ppt) for 96h. In both salinities, Cu transiently inhibited ammonia excretion (J amm) followed by a full recovery by the end of the 96h exposure. The activities of Na +/K +-ATPase, H +-ATPase, and carbonic anhydrase (CA) were examined in the gills at 12 and 96h of Cu exposure. In both salinity acclimations, CA activity was significantly inhibited following 12h of Cu exposure in P. vivipara, marking the first in vivo evidence of Cu-induced inhibition of CA in fish. Moreover, the inhibition and recovery of this enzyme were correlated with the inhibition and recovery of J amm in both salinity acclimations. The blockade of CA potentially acts as a common mechanism of J amm inhibition in FW and SW. There were no significant effects on Na +/K +-ATPase or H +-ATPase activity at either time point or salinity. However, H +-ATPase activity was upregulated at 96h relative to the 12h time point, potentially involving this enzyme in re-establishing J amm.

Original languageEnglish
Pages (from-to)172-180
Number of pages9
JournalAquatic Toxicology
Volume122-123
DOIs
StatePublished - Oct 15 2012

Fingerprint

Poecilia
Carbonic Anhydrases
Poecilia reticulata
carbonate dehydratase
Salinity
Seawater
Fresh Water
Ammonia
excretion
H-transporting ATP synthase
Proton-Translocating ATPases
Copper
ammonia
seawater
copper
salinity
sodium-potassium-exchanging ATPase
Acclimatization
acclimation
euryhaline species

Keywords

  • Ammonia excretion (J )
  • Carbonic anhydrase (CA)
  • Copper (Cu)
  • Euryhaline
  • Poecilia vivipara

ASJC Scopus subject areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

Cite this

Waterborne copper exposure inhibits ammonia excretion and branchial carbonic anhydrase activity in euryhaline guppies acclimated to both fresh water and sea water. / Zimmer, Alex M.; Barcarolli, Indianara Fernanda; Wood, Chris M.; Bianchini, Adalto.

In: Aquatic Toxicology, Vol. 122-123, 15.10.2012, p. 172-180.

Research output: Contribution to journalArticle

Zimmer, Alex M. ; Barcarolli, Indianara Fernanda ; Wood, Chris M. ; Bianchini, Adalto. / Waterborne copper exposure inhibits ammonia excretion and branchial carbonic anhydrase activity in euryhaline guppies acclimated to both fresh water and sea water. In: Aquatic Toxicology. 2012 ; Vol. 122-123. pp. 172-180.
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