Abstract
Inhibition of ammonia excretion (J amm) is a common response to Cu exposure in freshwater (FW) and seawater (SW) organisms. To determine the mechanism of this response, a euryhaline species of guppy (Poecilia vivipara) was exposed to 20μg Cu/l in FW (0ppt) and SW (25ppt) for 96h. In both salinities, Cu transiently inhibited ammonia excretion (J amm) followed by a full recovery by the end of the 96h exposure. The activities of Na +/K +-ATPase, H +-ATPase, and carbonic anhydrase (CA) were examined in the gills at 12 and 96h of Cu exposure. In both salinity acclimations, CA activity was significantly inhibited following 12h of Cu exposure in P. vivipara, marking the first in vivo evidence of Cu-induced inhibition of CA in fish. Moreover, the inhibition and recovery of this enzyme were correlated with the inhibition and recovery of J amm in both salinity acclimations. The blockade of CA potentially acts as a common mechanism of J amm inhibition in FW and SW. There were no significant effects on Na +/K +-ATPase or H +-ATPase activity at either time point or salinity. However, H +-ATPase activity was upregulated at 96h relative to the 12h time point, potentially involving this enzyme in re-establishing J amm.
| Original language | English |
|---|---|
| Pages (from-to) | 172-180 |
| Number of pages | 9 |
| Journal | Aquatic Toxicology |
| Volume | 122-123 |
| DOIs | |
| State | Published - Oct 15 2012 |
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Keywords
- Ammonia excretion (J )
- Carbonic anhydrase (CA)
- Copper (Cu)
- Euryhaline
- Poecilia vivipara
ASJC Scopus subject areas
- Aquatic Science
- Health, Toxicology and Mutagenesis
Cite this
Waterborne copper exposure inhibits ammonia excretion and branchial carbonic anhydrase activity in euryhaline guppies acclimated to both fresh water and sea water. / Zimmer, Alex M.; Barcarolli, Indianara Fernanda; Wood, Chris M.; Bianchini, Adalto.
In: Aquatic Toxicology, Vol. 122-123, 15.10.2012, p. 172-180.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Waterborne copper exposure inhibits ammonia excretion and branchial carbonic anhydrase activity in euryhaline guppies acclimated to both fresh water and sea water
AU - Zimmer, Alex M.
AU - Barcarolli, Indianara Fernanda
AU - Wood, Chris M.
AU - Bianchini, Adalto
PY - 2012/10/15
Y1 - 2012/10/15
N2 - Inhibition of ammonia excretion (J amm) is a common response to Cu exposure in freshwater (FW) and seawater (SW) organisms. To determine the mechanism of this response, a euryhaline species of guppy (Poecilia vivipara) was exposed to 20μg Cu/l in FW (0ppt) and SW (25ppt) for 96h. In both salinities, Cu transiently inhibited ammonia excretion (J amm) followed by a full recovery by the end of the 96h exposure. The activities of Na +/K +-ATPase, H +-ATPase, and carbonic anhydrase (CA) were examined in the gills at 12 and 96h of Cu exposure. In both salinity acclimations, CA activity was significantly inhibited following 12h of Cu exposure in P. vivipara, marking the first in vivo evidence of Cu-induced inhibition of CA in fish. Moreover, the inhibition and recovery of this enzyme were correlated with the inhibition and recovery of J amm in both salinity acclimations. The blockade of CA potentially acts as a common mechanism of J amm inhibition in FW and SW. There were no significant effects on Na +/K +-ATPase or H +-ATPase activity at either time point or salinity. However, H +-ATPase activity was upregulated at 96h relative to the 12h time point, potentially involving this enzyme in re-establishing J amm.
AB - Inhibition of ammonia excretion (J amm) is a common response to Cu exposure in freshwater (FW) and seawater (SW) organisms. To determine the mechanism of this response, a euryhaline species of guppy (Poecilia vivipara) was exposed to 20μg Cu/l in FW (0ppt) and SW (25ppt) for 96h. In both salinities, Cu transiently inhibited ammonia excretion (J amm) followed by a full recovery by the end of the 96h exposure. The activities of Na +/K +-ATPase, H +-ATPase, and carbonic anhydrase (CA) were examined in the gills at 12 and 96h of Cu exposure. In both salinity acclimations, CA activity was significantly inhibited following 12h of Cu exposure in P. vivipara, marking the first in vivo evidence of Cu-induced inhibition of CA in fish. Moreover, the inhibition and recovery of this enzyme were correlated with the inhibition and recovery of J amm in both salinity acclimations. The blockade of CA potentially acts as a common mechanism of J amm inhibition in FW and SW. There were no significant effects on Na +/K +-ATPase or H +-ATPase activity at either time point or salinity. However, H +-ATPase activity was upregulated at 96h relative to the 12h time point, potentially involving this enzyme in re-establishing J amm.
KW - Ammonia excretion (J )
KW - Carbonic anhydrase (CA)
KW - Copper (Cu)
KW - Euryhaline
KW - Poecilia vivipara
UR - http://www.scopus.com/inward/record.url?scp=84864054648&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864054648&partnerID=8YFLogxK
U2 - 10.1016/j.aquatox.2012.06.010
DO - 10.1016/j.aquatox.2012.06.010
M3 - Article
C2 - 22819806
AN - SCOPUS:84864054648
VL - 122-123
SP - 172
EP - 180
JO - Aquatic Toxicology
JF - Aquatic Toxicology
SN - 0166-445X
ER -