Water Immersion Stress Prevents Caerulein-induced Pancreatic Acinar Cell NF-κB Activation by Attenuating Caerulein-induced Intracellular Ca 2+ Changes

Antti J. Hietaranta, Vijay P. Singh, Lakshmi Bhagat, Gijs J.D. Van Acker, Albert M. Song, Andreas Mykoniatis, Michael L. Steer, Ashok K. Saluja

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Prior stress ameliorates caerulein-induced pancreatitis in rats. NF-κB is a proinflammatory transcription factor activated during caerulein pancreatitis. However, the effects of prior stress on pancreatic NF-κB activation are unknown. In the current study, the effect of prior water immersion stress on caerulein and tumor necrosis factor-α (TNF-α)-induced NF-κB activation in the pancreas was evaluated. Water immersion of rats for up to 6 h prevents supramaximal caerulein-induced pancreatic IκB-α degradation and NF-κB activation in vivo. NF-κB activity is also inhibited in vitro in pancreatic acini prepared from water-immersed animals. TNF-α-induced NF-κB activation in pancreas or in pancreatic acini is unaffected by prior water immersion. Chelation of intracellular Ca2+ by 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetate/acetoxymethyl ester has similar effects to water immersion in preventing caerulein but not TNF-α-induced NF-κB activation in pancreas. Both the spike response and the sustained rise in [Ca2+]i in response to supramaximal caerulein stimulation are reduced markedly in acini prepared from water-immersed animals as compared with normal animals. Our findings indicate that, in addition to Ca2+-dependent mechanisms, Ca 2+-independent signaling events also may lead to NF-κB activation in pancreatic acinar cells. Water immersion stress prevents supramaximal caerulein-induced NF-κB activation in pancreas in vivo and in vitro by affecting intracellular Ca2+ homeostasis.

Original languageEnglish (US)
Pages (from-to)18742-18747
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number22
DOIs
StatePublished - Jun 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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