One hundred and twenty excised rabbits hearts were subjected to 1 h of continuous pulsatile coronary perfusion with acellular fluids in a Lindbergh-Rockefeller Institution organ perfusion apparatus. Perfusions were carried out at 26°C and 15°C. At the end of 1 h of perfusion, samples of the left ventricular myocardium were packed into nuclear magnetic resonance (NMR) tubes and the water peak area and linewidth of the water signal were determined by the steady-state NMR spectroscopy. Seventeen different perfusates were employed. Four hearts were perfused and one control heart was processed in an identical way, with each set of experiments. Results were expressed as the mean percentage increase in total myocardial water in perfused hearts, as compared to unperfused hearts. At 26°C these ranged from 9.31 (s.e. 0.66) to 35.89 (s.e. 4.09). At 15°C the range was from 13.26 (s.e. 1.03) to 39.14 (s.e. 2.06). At 26°C, the mean linewidth (Hz) in control myocardiums was 11.60 (s.e. 0.40), in perfused hearts 7.34 (s.e. 0.33); at 15°C it was 12.15 (s.e. 0.29) in control hearts, and 7.84 (s.e. 0.37) in perfused ones. Hearts perfused with lactated Ringer's solution showed the greatest interstitial water accumulation. Linewidth narrowing indicated an accumulation of "unstructured" presumably extracellular water. In edematous hearts, interstitial spaces were widely dilated. The described technique can serve as a rapid screening method for assessing myocardial edema produced by perfusion.
- Heart perfusion
- Myocardial edema
- Nuclear magnetic resonance (NMR)
ASJC Scopus subject areas
- Molecular Biology
- Cardiology and Cardiovascular Medicine