Abstract
A new method is described which permits the measurement of membrane currents of thick muscle fibres (diameter 300 μm or more) of Astacus fluviatilis or Balanus balanus under voltage clamp conditions. The potential difference across a small patch of membrane (60-100 μm in diameter) is controlled by connecting a voltage source across it with two external electrodes. One of them is connected to the fluid bathing the muscle fibre. The other, tubular one is in touch with the test area. The current flowing through the electrodes represents the sum of the membrane current flowing across the test area and the leak current flowing in the external fluid between the electrodes. In the first version of the method the leak current is limited by a circular sucrose gap around the test area. In the second, more elaborate method, the leak current is eliminated by a system of two concentric sucrose rings with a guard ring electrode between them. This method permits in addition the measurement of full sized action potentials in the test area.
| Original language | English |
|---|---|
| Pages (from-to) | 71-79 |
| Number of pages | 9 |
| Journal | Pflügers Archiv European Journal of Physiology |
| Volume | 313 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 1 1969 |
| Externally published | Yes |
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Keywords
- Current Clamp
- Giant Muscle Fibre
- Membrane
- Sucrose Gap
- Voltage-Clamp
ASJC Scopus subject areas
- Physiology
Cite this
Voltage clamp of a small muscle membrane area by means of a circular sucrose gap arrangement. / Henček, M.; Nonner, W.; Stämpfli, R.
In: Pflügers Archiv European Journal of Physiology, Vol. 313, No. 1, 01.03.1969, p. 71-79.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Voltage clamp of a small muscle membrane area by means of a circular sucrose gap arrangement
AU - Henček, M.
AU - Nonner, W.
AU - Stämpfli, R.
PY - 1969/3/1
Y1 - 1969/3/1
N2 - A new method is described which permits the measurement of membrane currents of thick muscle fibres (diameter 300 μm or more) of Astacus fluviatilis or Balanus balanus under voltage clamp conditions. The potential difference across a small patch of membrane (60-100 μm in diameter) is controlled by connecting a voltage source across it with two external electrodes. One of them is connected to the fluid bathing the muscle fibre. The other, tubular one is in touch with the test area. The current flowing through the electrodes represents the sum of the membrane current flowing across the test area and the leak current flowing in the external fluid between the electrodes. In the first version of the method the leak current is limited by a circular sucrose gap around the test area. In the second, more elaborate method, the leak current is eliminated by a system of two concentric sucrose rings with a guard ring electrode between them. This method permits in addition the measurement of full sized action potentials in the test area.
AB - A new method is described which permits the measurement of membrane currents of thick muscle fibres (diameter 300 μm or more) of Astacus fluviatilis or Balanus balanus under voltage clamp conditions. The potential difference across a small patch of membrane (60-100 μm in diameter) is controlled by connecting a voltage source across it with two external electrodes. One of them is connected to the fluid bathing the muscle fibre. The other, tubular one is in touch with the test area. The current flowing through the electrodes represents the sum of the membrane current flowing across the test area and the leak current flowing in the external fluid between the electrodes. In the first version of the method the leak current is limited by a circular sucrose gap around the test area. In the second, more elaborate method, the leak current is eliminated by a system of two concentric sucrose rings with a guard ring electrode between them. This method permits in addition the measurement of full sized action potentials in the test area.
KW - Current Clamp
KW - Giant Muscle Fibre
KW - Membrane
KW - Sucrose Gap
KW - Voltage-Clamp
UR - http://www.scopus.com/inward/record.url?scp=0014629655&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0014629655&partnerID=8YFLogxK
U2 - 10.1007/BF00586330
DO - 10.1007/BF00586330
M3 - Article
C2 - 5389972
AN - SCOPUS:0014629655
VL - 313
SP - 71
EP - 79
JO - Pflugers Archiv European Journal of Physiology
JF - Pflugers Archiv European Journal of Physiology
SN - 0031-6768
IS - 1
ER -