Voltage clamp of a small muscle membrane area by means of a circular sucrose gap arrangement

M. Henček, W. Nonner, R. Stämpfli

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A new method is described which permits the measurement of membrane currents of thick muscle fibres (diameter 300 μm or more) of Astacus fluviatilis or Balanus balanus under voltage clamp conditions. The potential difference across a small patch of membrane (60-100 μm in diameter) is controlled by connecting a voltage source across it with two external electrodes. One of them is connected to the fluid bathing the muscle fibre. The other, tubular one is in touch with the test area. The current flowing through the electrodes represents the sum of the membrane current flowing across the test area and the leak current flowing in the external fluid between the electrodes. In the first version of the method the leak current is limited by a circular sucrose gap around the test area. In the second, more elaborate method, the leak current is eliminated by a system of two concentric sucrose rings with a guard ring electrode between them. This method permits in addition the measurement of full sized action potentials in the test area.

Original languageEnglish
Pages (from-to)71-79
Number of pages9
JournalPflügers Archiv European Journal of Physiology
Volume313
Issue number1
DOIs
StatePublished - Mar 1 1969
Externally publishedYes

Fingerprint

Clamping devices
Sucrose
Muscle
Electrodes
Thoracica
Membranes
Muscles
Electric potential
Astacoidea
Fluids
Fibers
Action Potentials

Keywords

  • Current Clamp
  • Giant Muscle Fibre
  • Membrane
  • Sucrose Gap
  • Voltage-Clamp

ASJC Scopus subject areas

  • Physiology

Cite this

Voltage clamp of a small muscle membrane area by means of a circular sucrose gap arrangement. / Henček, M.; Nonner, W.; Stämpfli, R.

In: Pflügers Archiv European Journal of Physiology, Vol. 313, No. 1, 01.03.1969, p. 71-79.

Research output: Contribution to journalArticle

@article{a61ccb2638444391aaba7b58a96ad7a4,
title = "Voltage clamp of a small muscle membrane area by means of a circular sucrose gap arrangement",
abstract = "A new method is described which permits the measurement of membrane currents of thick muscle fibres (diameter 300 μm or more) of Astacus fluviatilis or Balanus balanus under voltage clamp conditions. The potential difference across a small patch of membrane (60-100 μm in diameter) is controlled by connecting a voltage source across it with two external electrodes. One of them is connected to the fluid bathing the muscle fibre. The other, tubular one is in touch with the test area. The current flowing through the electrodes represents the sum of the membrane current flowing across the test area and the leak current flowing in the external fluid between the electrodes. In the first version of the method the leak current is limited by a circular sucrose gap around the test area. In the second, more elaborate method, the leak current is eliminated by a system of two concentric sucrose rings with a guard ring electrode between them. This method permits in addition the measurement of full sized action potentials in the test area.",
keywords = "Current Clamp, Giant Muscle Fibre, Membrane, Sucrose Gap, Voltage-Clamp",
author = "M. Henček and W. Nonner and R. St{\"a}mpfli",
year = "1969",
month = "3",
day = "1",
doi = "10.1007/BF00586330",
language = "English",
volume = "313",
pages = "71--79",
journal = "Pflugers Archiv European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Voltage clamp of a small muscle membrane area by means of a circular sucrose gap arrangement

AU - Henček, M.

AU - Nonner, W.

AU - Stämpfli, R.

PY - 1969/3/1

Y1 - 1969/3/1

N2 - A new method is described which permits the measurement of membrane currents of thick muscle fibres (diameter 300 μm or more) of Astacus fluviatilis or Balanus balanus under voltage clamp conditions. The potential difference across a small patch of membrane (60-100 μm in diameter) is controlled by connecting a voltage source across it with two external electrodes. One of them is connected to the fluid bathing the muscle fibre. The other, tubular one is in touch with the test area. The current flowing through the electrodes represents the sum of the membrane current flowing across the test area and the leak current flowing in the external fluid between the electrodes. In the first version of the method the leak current is limited by a circular sucrose gap around the test area. In the second, more elaborate method, the leak current is eliminated by a system of two concentric sucrose rings with a guard ring electrode between them. This method permits in addition the measurement of full sized action potentials in the test area.

AB - A new method is described which permits the measurement of membrane currents of thick muscle fibres (diameter 300 μm or more) of Astacus fluviatilis or Balanus balanus under voltage clamp conditions. The potential difference across a small patch of membrane (60-100 μm in diameter) is controlled by connecting a voltage source across it with two external electrodes. One of them is connected to the fluid bathing the muscle fibre. The other, tubular one is in touch with the test area. The current flowing through the electrodes represents the sum of the membrane current flowing across the test area and the leak current flowing in the external fluid between the electrodes. In the first version of the method the leak current is limited by a circular sucrose gap around the test area. In the second, more elaborate method, the leak current is eliminated by a system of two concentric sucrose rings with a guard ring electrode between them. This method permits in addition the measurement of full sized action potentials in the test area.

KW - Current Clamp

KW - Giant Muscle Fibre

KW - Membrane

KW - Sucrose Gap

KW - Voltage-Clamp

UR - http://www.scopus.com/inward/record.url?scp=0014629655&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014629655&partnerID=8YFLogxK

U2 - 10.1007/BF00586330

DO - 10.1007/BF00586330

M3 - Article

C2 - 5389972

AN - SCOPUS:0014629655

VL - 313

SP - 71

EP - 79

JO - Pflugers Archiv European Journal of Physiology

JF - Pflugers Archiv European Journal of Physiology

SN - 0031-6768

IS - 1

ER -