Vitamin E succinate inhibits NF-κB and prevents the development of a metastatic phenotype in prostate cancer cells

Implications for chemoprevention

Paul L. Crispen, Robert G. Uzzo, Konstantin Golovine, Peter Makhov, Alan Pollack, Eric M. Horwitz, Richard E. Greenberg, Vladimir M. Kolenko

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

BACKGROUND. NF-κB and AP-1 transcriptional factors contribute to the development and progression of prostate malignancy by regulating the expression of genes involved in proliferation, apoptosis, angiogenesis, and metastasis. METHODS. NF-κB and AP-1 activities were examined by TransAm assay. Cytokines levels were assessed by ELISA. ICAM-1 and gp130 expression was examined by flow cytometry. Cell adhesion was examined by the ability of cells to adhere to fibronectin-coated plates. Cell viability was determined by propidium iodide staining. RESULTS. Treatment with α-tocopherol succinate (VES) inhibits NF-κB but augments AP-1 activity, reduces expression of IL-6, IL-8, and VEGF, suppresses cell adhesion, ICAM-1 and gp130 expression in androgen-independent PC-3, DU-145, and CA-HPV-10 cells. VES supplementation also decreases the expression of anti-apoptotic XIAP and Bcl-XL proteins and sensitizes androgen-dependent LNCaP cells to androgen deprivation. CONCLUSIONS. Our findings propose a potential mechanism of VES-mediated anti-tumor activity and support the role of vitamin E analogs as potential chemopreventative agents against prostate cancer.

Original languageEnglish
Pages (from-to)582-590
Number of pages9
JournalProstate
Volume67
Issue number6
DOIs
StatePublished - May 1 2007
Externally publishedYes

Fingerprint

Transcription Factor AP-1
Chemoprevention
Succinic Acid
Vitamin E
Androgens
Prostatic Neoplasms
Intercellular Adhesion Molecule-1
Phenotype
Cell Adhesion
bcl-X Protein
Propidium
alpha-Tocopherol
Interleukin-8
Fibronectins
Vascular Endothelial Growth Factor A
Prostate
Interleukin-6
Neoplasms
Cell Survival
Flow Cytometry

Keywords

  • Adhesion
  • AP-1
  • Apoptosis
  • Cytokines

ASJC Scopus subject areas

  • Urology

Cite this

Vitamin E succinate inhibits NF-κB and prevents the development of a metastatic phenotype in prostate cancer cells : Implications for chemoprevention. / Crispen, Paul L.; Uzzo, Robert G.; Golovine, Konstantin; Makhov, Peter; Pollack, Alan; Horwitz, Eric M.; Greenberg, Richard E.; Kolenko, Vladimir M.

In: Prostate, Vol. 67, No. 6, 01.05.2007, p. 582-590.

Research output: Contribution to journalArticle

Crispen, Paul L. ; Uzzo, Robert G. ; Golovine, Konstantin ; Makhov, Peter ; Pollack, Alan ; Horwitz, Eric M. ; Greenberg, Richard E. ; Kolenko, Vladimir M. / Vitamin E succinate inhibits NF-κB and prevents the development of a metastatic phenotype in prostate cancer cells : Implications for chemoprevention. In: Prostate. 2007 ; Vol. 67, No. 6. pp. 582-590.
@article{64e4628e3e314617b397faa65aa74400,
title = "Vitamin E succinate inhibits NF-κB and prevents the development of a metastatic phenotype in prostate cancer cells: Implications for chemoprevention",
abstract = "BACKGROUND. NF-κB and AP-1 transcriptional factors contribute to the development and progression of prostate malignancy by regulating the expression of genes involved in proliferation, apoptosis, angiogenesis, and metastasis. METHODS. NF-κB and AP-1 activities were examined by TransAm assay. Cytokines levels were assessed by ELISA. ICAM-1 and gp130 expression was examined by flow cytometry. Cell adhesion was examined by the ability of cells to adhere to fibronectin-coated plates. Cell viability was determined by propidium iodide staining. RESULTS. Treatment with α-tocopherol succinate (VES) inhibits NF-κB but augments AP-1 activity, reduces expression of IL-6, IL-8, and VEGF, suppresses cell adhesion, ICAM-1 and gp130 expression in androgen-independent PC-3, DU-145, and CA-HPV-10 cells. VES supplementation also decreases the expression of anti-apoptotic XIAP and Bcl-XL proteins and sensitizes androgen-dependent LNCaP cells to androgen deprivation. CONCLUSIONS. Our findings propose a potential mechanism of VES-mediated anti-tumor activity and support the role of vitamin E analogs as potential chemopreventative agents against prostate cancer.",
keywords = "Adhesion, AP-1, Apoptosis, Cytokines",
author = "Crispen, {Paul L.} and Uzzo, {Robert G.} and Konstantin Golovine and Peter Makhov and Alan Pollack and Horwitz, {Eric M.} and Greenberg, {Richard E.} and Kolenko, {Vladimir M.}",
year = "2007",
month = "5",
day = "1",
doi = "10.1002/pros.20468",
language = "English",
volume = "67",
pages = "582--590",
journal = "Prostate",
issn = "0270-4137",
publisher = "Wiley-Liss Inc.",
number = "6",

}

TY - JOUR

T1 - Vitamin E succinate inhibits NF-κB and prevents the development of a metastatic phenotype in prostate cancer cells

T2 - Implications for chemoprevention

AU - Crispen, Paul L.

AU - Uzzo, Robert G.

AU - Golovine, Konstantin

AU - Makhov, Peter

AU - Pollack, Alan

AU - Horwitz, Eric M.

AU - Greenberg, Richard E.

AU - Kolenko, Vladimir M.

PY - 2007/5/1

Y1 - 2007/5/1

N2 - BACKGROUND. NF-κB and AP-1 transcriptional factors contribute to the development and progression of prostate malignancy by regulating the expression of genes involved in proliferation, apoptosis, angiogenesis, and metastasis. METHODS. NF-κB and AP-1 activities were examined by TransAm assay. Cytokines levels were assessed by ELISA. ICAM-1 and gp130 expression was examined by flow cytometry. Cell adhesion was examined by the ability of cells to adhere to fibronectin-coated plates. Cell viability was determined by propidium iodide staining. RESULTS. Treatment with α-tocopherol succinate (VES) inhibits NF-κB but augments AP-1 activity, reduces expression of IL-6, IL-8, and VEGF, suppresses cell adhesion, ICAM-1 and gp130 expression in androgen-independent PC-3, DU-145, and CA-HPV-10 cells. VES supplementation also decreases the expression of anti-apoptotic XIAP and Bcl-XL proteins and sensitizes androgen-dependent LNCaP cells to androgen deprivation. CONCLUSIONS. Our findings propose a potential mechanism of VES-mediated anti-tumor activity and support the role of vitamin E analogs as potential chemopreventative agents against prostate cancer.

AB - BACKGROUND. NF-κB and AP-1 transcriptional factors contribute to the development and progression of prostate malignancy by regulating the expression of genes involved in proliferation, apoptosis, angiogenesis, and metastasis. METHODS. NF-κB and AP-1 activities were examined by TransAm assay. Cytokines levels were assessed by ELISA. ICAM-1 and gp130 expression was examined by flow cytometry. Cell adhesion was examined by the ability of cells to adhere to fibronectin-coated plates. Cell viability was determined by propidium iodide staining. RESULTS. Treatment with α-tocopherol succinate (VES) inhibits NF-κB but augments AP-1 activity, reduces expression of IL-6, IL-8, and VEGF, suppresses cell adhesion, ICAM-1 and gp130 expression in androgen-independent PC-3, DU-145, and CA-HPV-10 cells. VES supplementation also decreases the expression of anti-apoptotic XIAP and Bcl-XL proteins and sensitizes androgen-dependent LNCaP cells to androgen deprivation. CONCLUSIONS. Our findings propose a potential mechanism of VES-mediated anti-tumor activity and support the role of vitamin E analogs as potential chemopreventative agents against prostate cancer.

KW - Adhesion

KW - AP-1

KW - Apoptosis

KW - Cytokines

UR - http://www.scopus.com/inward/record.url?scp=34247571820&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247571820&partnerID=8YFLogxK

U2 - 10.1002/pros.20468

DO - 10.1002/pros.20468

M3 - Article

VL - 67

SP - 582

EP - 590

JO - Prostate

JF - Prostate

SN - 0270-4137

IS - 6

ER -