Vitamin E attenuates induction of elastase-like activity by tumor necrosis factor-α, cholestan-3β,5α,6β-triol and linoleic acid in cultured endothelial cells

Michal Toborek, Bernhard Hennig

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Disturbances in arterial wall elastin metabolism appear to be important factors in atherosclerosis development. To evaluate this hypothesis, elastase-like activity was determined in cultured endothelial cells and their surrounding media after exposure to tumor necrosis factor-α (TNF), cholestan-3β,5α,6β-triol (Triol) and linoleic acid (18:2). Significant increases in elastase-like activity both in the cells and in the media were observed when subconfluent endothelial cells were treated with 12 μM Triol, 500 U TNF/ml, or 90 μM 18:2, for 72 h in the presence of 5% calf serum. Even higher activities were measured when endothelial cells were seeded directly into media enriched with 18:2, TNF or Triol and treated for 72 h. Vitamin E supplementation (25 μM) attenuated elastase-like activity in cells and media, independent of treatment. These results suggest that elastase-like enzyme induction in endothelial cells may be involved in cellular pertubations induced by certain lipids and cytokines. Vitamin E may provide a protective function by preventing the induction of elastolytic enzymes. This may have implications in elastin metabolism and atherosclerosis.

Original languageEnglish (US)
Pages (from-to)201-211
Number of pages11
JournalClinica Chimica Acta
Volume215
Issue number2
DOIs
StatePublished - Jun 16 1993
Externally publishedYes

Keywords

  • Cholestan-3β,5α,6β-triol
  • Elastase-like activity
  • Endothelial cells
  • Linoleic acid
  • Tumor necrosis factor-α
  • Vitamin E

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Vitamin E attenuates induction of elastase-like activity by tumor necrosis factor-α, cholestan-3β,5α,6β-triol and linoleic acid in cultured endothelial cells'. Together they form a unique fingerprint.

  • Cite this