Vitamin D in plasma: quantitation by a nonequilibrium ligand binding assay

Bruce W. Hollis, Bernard A. Roos, Phillip W. Lambert

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The concentration of vitamin D was determined in human and bovine plasma samples under various physiological and nonphysiological conditions using a nonequilibrium ligand binding assay. Prior to ligand binding analysis the vitamin D in the plasma organic extracts was purified using chromatographic procedures involving Lipidex-5000 and high performance liquid chromatography. The use of a nonequilibrium assay system greatly increased the sensitivity of our assay allowing for a minimum volume of the initial plasma sample. The vitamin D levels in plasma responded to increased sun exposure as well as to the intoxication with vitamin D3. Analysis of a plasma sample from a vitamin D-deficient patient revealed that lipid interference was not a factor in this ligand binding assay.

Original languageEnglish
Pages (from-to)609-619
Number of pages11
JournalSteroids
Volume37
Issue number6
DOIs
StatePublished - Jan 1 1981
Externally publishedYes

Fingerprint

Vitamin D
Assays
Ligands
Plasmas
Plasma Volume
Cholecalciferol
Solar System
High performance liquid chromatography
Sun
High Pressure Liquid Chromatography
Lipids

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Molecular Biology

Cite this

Vitamin D in plasma : quantitation by a nonequilibrium ligand binding assay. / Hollis, Bruce W.; Roos, Bernard A.; Lambert, Phillip W.

In: Steroids, Vol. 37, No. 6, 01.01.1981, p. 609-619.

Research output: Contribution to journalArticle

Hollis, Bruce W. ; Roos, Bernard A. ; Lambert, Phillip W. / Vitamin D in plasma : quantitation by a nonequilibrium ligand binding assay. In: Steroids. 1981 ; Vol. 37, No. 6. pp. 609-619.
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