Visualization of the binding site for the transcript cleavage factor GreB on Escherichia coli RNA polymerase

Andrey Polyakov, Catherine Richter, Arun Malhotra, Dmitry Koulich, Sergei Borukhov, Seth A. Darst

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The structure of Escherichia coli core RNA polymerase (RNAP) complexed with the transcript cleavage factor GreB was determined from electron micrographs of negatively stained, flattened helical crystals. A binding assay was developed to establish that GreB was incorporated into the RNA polymerase crystals with high occupancy through interactions between the globular C-terminal domain and the RNA polymerase. Comparison of the core RNAP:GreB structure with the previously determined structure of core RNAP located the GreB binding site on one face of the RNA polymerase, next to but not in the 25 Å-diameter channel of RNA polymerase.

Original languageEnglish (US)
Pages (from-to)465-473
Number of pages9
JournalJournal of molecular biology
Volume281
Issue number3
DOIs
StatePublished - Aug 21 1998
Externally publishedYes

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Keywords

  • E. coli RNA polymerase
  • Electron microscopy
  • GreB
  • Transcript cleavage factor
  • Transcription elongation factor

ASJC Scopus subject areas

  • Virology

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