Visualization and analysis of mural thrombogenesis on collagen, polyurethane and nylon

Jeffrey A. Hubbell, Larry V. McIntire

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

Visualization and analysis of mural thrombogenesis on collagen-coated glass, polyurethane and nylon was discussed. Epi-fluorescent video microscopy was used to visualize thrombotic events at a protein or polymer surface in contact with flowing whole blood. Digital image processing was used to analyse the real-time microscopic images obtained, resulting in measurements of morphological features and the number of platelets that compose each thrombus. The three-dimensional structures of the thrombi were also estimated. A photodiode was used to measure integrated end-point platelet accumulation at different axial positions along the surface. Additionally, the convection-diffusion equations were solved to estimate the concentrations of adenosine diphosphate, thromboxane Az, and thrombin generated by activated platelets at the blood-contacting surface.

Original languageEnglish (US)
Pages (from-to)354-360,IN1,361-363
JournalBiomaterials
Volume7
Issue number5
DOIs
StatePublished - Sep 1986

Keywords

  • Blood
  • collagen
  • digital image processing
  • fluorescence microscopy
  • mural thrombogenesis
  • nylon
  • polyurethane
  • thrombosis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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