Visualization and analysis of mural thrombogenesis on collagen, polyurethane and nylon

Jeffrey A. Hubbell, Larry V. McIntire

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Visualization and analysis of mural thrombogenesis on collagen-coated glass, polyurethane and nylon was discussed. Epi-fluorescent video microscopy was used to visualize thrombotic events at a protein or polymer surface in contact with flowing whole blood. Digital image processing was used to analyse the real-time microscopic images obtained, resulting in measurements of morphological features and the number of platelets that compose each thrombus. The three-dimensional structures of the thrombi were also estimated. A photodiode was used to measure integrated end-point platelet accumulation at different axial positions along the surface. Additionally, the convection-diffusion equations were solved to estimate the concentrations of adenosine diphosphate, thromboxane Az, and thrombin generated by activated platelets at the blood-contacting surface.

Original languageEnglish
JournalBiomaterials
Volume7
Issue number5
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

Polyurethanes
Nylons
Platelets
Collagen
Thrombosis
Blood Platelets
Visualization
Video Microscopy
Convection
Thromboxanes
Blood
Platelet Count
Thrombin
Adenosine Diphosphate
Glass
Polymers
Photodiodes
Microscopic examination
Image processing
Proteins

Keywords

  • Blood
  • collagen
  • digital image processing
  • fluorescence microscopy
  • mural thrombogenesis
  • nylon
  • polyurethane
  • thrombosis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Visualization and analysis of mural thrombogenesis on collagen, polyurethane and nylon. / Hubbell, Jeffrey A.; McIntire, Larry V.

In: Biomaterials, Vol. 7, No. 5, 01.01.1986.

Research output: Contribution to journalArticle

Hubbell, Jeffrey A. ; McIntire, Larry V. / Visualization and analysis of mural thrombogenesis on collagen, polyurethane and nylon. In: Biomaterials. 1986 ; Vol. 7, No. 5.
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