Visible-light optical coherence tomography-based multimodal system for quantitative fundus autofluorescence imaging

Zahra Nafar, Rong Wen, Shuliang Jiao

Research output: Contribution to journalReview article

Abstract

Fundus autofluorescence (FAF) imaging is commonly used in ophthalmic clinics for diagnosis and monitoring of retinal diseases. Lipofuscin in the retinal pigment epithelium (RPE), with A2E as its most abundant component and a visual cycle by-product, is the major fluorophore of FAF. Lipofuscin accumulates with age and is implicated in degenerative retinal diseases. The amount of lipofuscin in RPE can be assessed by quantitative measurement of FAF. However, the currently available FAF imaging technologies are not capable of quantifying the absolute intensity of FAF, which is essential for comparing images from different individuals, and from the same individual over time. One major technical difficulty is to compensate the signal attenuation by ocular media anterior to the RPE (pre-RPE media). FAF intensity is also influenced by fluctuations in imaging conditions such as illumination power and detector sensitivity, all of which need to be compensated. In this review, we present the concept and research progress of using visible-light optical coherence tomography-based simultaneous multimodal retinal imaging to compensate signal attenuation by pre-RPE media and the influence of parameters of the acquisition system for accurate measurement of FAF intensities. Impact statement: Quantitative fundus autofluorescence imaging with simultaneous visible-light optical coherence tomography-based multimodal technology has potential significant impact on the diagnosis and monitoring the progression of retinal diseases.

Original languageEnglish (US)
Pages (from-to)1265-1274
Number of pages10
JournalExperimental Biology and Medicine
Volume243
Issue number17-18
DOIs
StatePublished - Dec 1 2018

Fingerprint

Retinal Pigments
Retinal Pigment Epithelium
Optical Imaging
Optical tomography
Optical Coherence Tomography
Lipofuscin
Retinal Diseases
Imaging techniques
Light
Multimodal Imaging
Technology
Fluorophores
Monitoring
Lighting
Byproducts
Detectors
Research

Keywords

  • fluorescence quantification
  • fundus autofluorescence imaging
  • multimodal imaging
  • retinal imaging
  • retinal pigment epithelium lipofuscin
  • Visible-light optical coherence tomography

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Visible-light optical coherence tomography-based multimodal system for quantitative fundus autofluorescence imaging. / Nafar, Zahra; Wen, Rong; Jiao, Shuliang.

In: Experimental Biology and Medicine, Vol. 243, No. 17-18, 01.12.2018, p. 1265-1274.

Research output: Contribution to journalReview article

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abstract = "Fundus autofluorescence (FAF) imaging is commonly used in ophthalmic clinics for diagnosis and monitoring of retinal diseases. Lipofuscin in the retinal pigment epithelium (RPE), with A2E as its most abundant component and a visual cycle by-product, is the major fluorophore of FAF. Lipofuscin accumulates with age and is implicated in degenerative retinal diseases. The amount of lipofuscin in RPE can be assessed by quantitative measurement of FAF. However, the currently available FAF imaging technologies are not capable of quantifying the absolute intensity of FAF, which is essential for comparing images from different individuals, and from the same individual over time. One major technical difficulty is to compensate the signal attenuation by ocular media anterior to the RPE (pre-RPE media). FAF intensity is also influenced by fluctuations in imaging conditions such as illumination power and detector sensitivity, all of which need to be compensated. In this review, we present the concept and research progress of using visible-light optical coherence tomography-based simultaneous multimodal retinal imaging to compensate signal attenuation by pre-RPE media and the influence of parameters of the acquisition system for accurate measurement of FAF intensities. Impact statement: Quantitative fundus autofluorescence imaging with simultaneous visible-light optical coherence tomography-based multimodal technology has potential significant impact on the diagnosis and monitoring the progression of retinal diseases.",
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