Visible-light optical coherence tomography-based multimodal retinal imaging for improvement of fluorescent intensity quantification

Zahra Nafar, Minshan Jiang, Rong Wen, Shuliang Jiao

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

We developed a spectral-domain visible-light optical coherence tomography (VISOCT) based multimodal imaging technique which can accomplish simultaneous OCT and fluorescence imaging with a single broadband light source. Phantom experiments showed that by using the simultaneously acquired OCT images as a reference, the effect of light attenuation on the intensity of the fluorescent images by materials in front of the fluorescent target can be compensated. This capability of the multimodal imaging technique is of high importance for achieving quantification of the true intensities of autofluorescence (AF) imaging of the retina. We applied the technique in retinal imaging including AF imaging of the retinal pigment epithelium and fluorescein angiography (FA). We successfully demonstrated the effect of compensation on AF and FA images with the simultaneously acquired VIS-OCT images.

Original languageEnglish (US)
Article number#268335
Pages (from-to)3220-3229
Number of pages10
JournalBiomedical Optics Express
Volume7
Issue number9
DOIs
StatePublished - Sep 1 2016

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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