Virus-encoded cyclin

Jae U. Jung, Marion Stäger, Ronald Charles Desrosiers

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Herpesvirus saimiri contains an open reading frame called eclf2 with homology to the cellular type D cyclins. We now show that the eclf2 gene product is a novel virus-encoded cyclin (v-cyclin). The protein encoded by the v-cyclin gene of this oncogenic herpesvirus was found to have an apparent molecular size of 29 kDa in transformed cells. v-Cyclin protein was found to be associated with cdk6, a cellular cyclin-dependent kinase known to interact with cellular type D cyclins. cdk6/v-cyclin complexes strongly phosphorylated Rb fusion protein and histone H1 as substrates in vitro. Mutational analyses showed that highly conserved amino acids in the cyclin box of v-cyclin were important for association with cdk6 and for activation of cdk6 kinase activity. Thus, v-cyclin resembles cellular type D cyclins in primary sequence, in its association with cdk6, by its ability to activate protein kinase activity, and by the presence of functional cyclin box sequences. v- Cyclin exhibited a selective preference for association with cdk6 over other cyclin-dependent kinases and a high level of kinase activation. The properties of v-cyclin suggest a likely role in oncogenic transformation by this T-lymphotropic herpesvirus.

Original languageEnglish (US)
Pages (from-to)7235-7244
Number of pages10
JournalMolecular and Cellular Biology
Volume14
Issue number11
StatePublished - Nov 1994
Externally publishedYes

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Cyclins
Viruses
Cyclin D
Cyclin-Dependent Kinases
Herpesviridae
Phosphotransferases
Saimiriine Herpesvirus 2
Retinoblastoma Protein
Histones
Protein Kinases
Open Reading Frames
Genes
Proteins
Amino Acids

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Jung, J. U., Stäger, M., & Desrosiers, R. C. (1994). Virus-encoded cyclin. Molecular and Cellular Biology, 14(11), 7235-7244.

Virus-encoded cyclin. / Jung, Jae U.; Stäger, Marion; Desrosiers, Ronald Charles.

In: Molecular and Cellular Biology, Vol. 14, No. 11, 11.1994, p. 7235-7244.

Research output: Contribution to journalArticle

Jung, JU, Stäger, M & Desrosiers, RC 1994, 'Virus-encoded cyclin', Molecular and Cellular Biology, vol. 14, no. 11, pp. 7235-7244.
Jung JU, Stäger M, Desrosiers RC. Virus-encoded cyclin. Molecular and Cellular Biology. 1994 Nov;14(11):7235-7244.
Jung, Jae U. ; Stäger, Marion ; Desrosiers, Ronald Charles. / Virus-encoded cyclin. In: Molecular and Cellular Biology. 1994 ; Vol. 14, No. 11. pp. 7235-7244.
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