Vinyl chloride metabolites potentiate inflammatory liver injury caused by LPS in mice

Lisanne C. Anders, Anna L. Lang, Anwar Anwar-Mohamed, Amanda N. Douglas, Adrienne M. Bushau, Keith Cameron Falkner, Bradford G. Hill, Nikole L. Warner, Gavin E. Arteel, Matt Cave, Craig J. McClain, Juliane I. Beier

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Vinyl chloride (VC) is a ubiquitous environmental contaminant for which human risk is incompletely understood. We have previously reported that high occupational exposure to VC directly caused liver damage in humans. However, whether VC may also potentiate liver injury from other causes is not known. C57Bl/6J mice were administered chloroethanol (CE), a major metabolite of VC, and lipopolysaccharide (LPS) 24h after CE. Samples were harvested for determination of liver damage, inflammation, and changes in carbohydrate and lipid metabolism. In mice, CE exposure alone caused no detectable liver damage. LPS exposure caused inflammatory liver damage, oxidative stress, lipid accumulation, and glycogen depletion; the effect of all of these variables was potentiated by CE pre-exposure. In vitro experiments suggest that VC metabolite chloroacetaldehyde (CAA) directly damages mitochondria, which may explain the sensitization effect observed in vivo. Moreover, co-exposure of cells to CAA and TNFα caused increased cell death, supporting the hypothesis of sensitization by VC metabolites. Taken together, these data demonstrate that exposure to VC/metabolites at levels that are not overtly hepatotoxic can potentiate liver injury caused by another hepatotoxicant. This serves as proof-of-concept that VC hepatotoxicity may be modified by an additional metabolic stress such as endotoxemia, which commonly occurs in acute (eg, sepsis) and chronic (eg, NAFLD) diseases.

Original languageEnglish (US)
Pages (from-to)312-323
Number of pages12
JournalToxicological Sciences
Volume151
Issue number2
DOIs
StatePublished - Jun 2016
Externally publishedYes

Keywords

  • Hepatotoxicity
  • PVC
  • TASH
  • Toxicant-associated steatohepatitis
  • Vinyl chloride metabolite

ASJC Scopus subject areas

  • Toxicology

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