Vessel microport technique for applications in cerebrovascular research

Lei Chen, Karin R. Swartz, Michal J Toborek

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Cerebrovascular research suffers from a lack of reliable methods with which to deliver exogenous substances effectively into the central nervous system (CNS) of small experimental animals. Here we describe a novel vessel microport surgical technique for a variety of cerebrovascular applications that is reproducible and well tolerated in mice. The procedure is based on the insertion of a vessel microport into the external carotid artery for substance delivery into the CNS via the internal carotid artery. The method results in selective substance delivery into the ipsilateral hemisphere. Other novel aspects of this surgical technique include the ability to perform multiple injections, study of conscious mice well removed from surgery, and lack of occlusion of the common or internal carotid artery that allows carotid flow to be maintained. The feasibility of this technique has been validated by infusion of HIV Tat protein to induce permeability of the blood-brain barrier and by implantation of tumor cells to establish a brain metastasis model. Thus, the described vessel microport technique can be employed in a variety of cerebrovascular research applications.

Original languageEnglish
Pages (from-to)1718-1727
Number of pages10
JournalJournal of Neuroscience Research
Volume87
Issue number7
DOIs
StatePublished - May 15 2009
Externally publishedYes

Fingerprint

Internal Carotid Artery
Central Nervous System
Human Immunodeficiency Virus tat Gene Products
Research
External Carotid Artery
Common Carotid Artery
Blood-Brain Barrier
Permeability
Neoplasm Metastasis
Injections
Brain
Neoplasms

Keywords

  • Brain metastasis
  • CNS drug delivery
  • HIV Tat protein
  • Mouse carotid artery surgery
  • Vessel port

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Vessel microport technique for applications in cerebrovascular research. / Chen, Lei; Swartz, Karin R.; Toborek, Michal J.

In: Journal of Neuroscience Research, Vol. 87, No. 7, 15.05.2009, p. 1718-1727.

Research output: Contribution to journalArticle

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