Vesicular stomatitis virus inhibits mitotic progression and triggers cell death

Papia Chakraborty, Joachim Seemann, Ram K. Mishra, Jen Hsuan Wei, Lauren Weil, Daniel R. Nussenzveig, Joshua Heiber, Glen N Barber, Mary Dasso, Beatriz M A Fontoura

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Vesicular stomatitis virus (VSV) infects and kills a wide range of cell types; however, the mechanisms involved in VSV-mediated cell death are not fully understood. Here we show that VSV infection interferes with mitotic progression, resulting in cell death. This effect requires the interaction of VSV matrix (M) protein with the Rae1-Nup98 complex in mitosis, which is associated with a subset of ribonucleoproteins (RNPs). VSV displaced Rae1 from spindle poles, caused spindle abnormalities and triggered substantial cell death during metaphase. These effects were attenuated in cells infected with VSV expressing a mutant M protein that does not bind efficiently to the Rae1-Nup98-RNP complex. In cells that progressed to late mitosis, M protein prevented proper nuclear formation and chromatin decondensation. VSV is an oncolytic (anti-tumour) agent as it preferentially replicates and kills tumour cells. As tumour cells have a high mitotic index, VSV-mediated mitotic cell death probably contributes to its oncolytic activity.

Original languageEnglish
Pages (from-to)1154-1160
Number of pages7
JournalEMBO Reports
Volume10
Issue number10
DOIs
StatePublished - Sep 14 2009

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Vesicular Stomatitis
Cell death
Viruses
Cell Death
Ribonucleoproteins
Tumors
Mitosis
Oncolytic Viruses
Spindle Poles
Cells
Neoplasms
Mitotic Index
Proteins
Virus Diseases
Mutant Proteins
Metaphase
Chromatin
Poles

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry

Cite this

Chakraborty, P., Seemann, J., Mishra, R. K., Wei, J. H., Weil, L., Nussenzveig, D. R., ... Fontoura, B. M. A. (2009). Vesicular stomatitis virus inhibits mitotic progression and triggers cell death. EMBO Reports, 10(10), 1154-1160. https://doi.org/10.1038/embor.2009.179

Vesicular stomatitis virus inhibits mitotic progression and triggers cell death. / Chakraborty, Papia; Seemann, Joachim; Mishra, Ram K.; Wei, Jen Hsuan; Weil, Lauren; Nussenzveig, Daniel R.; Heiber, Joshua; Barber, Glen N; Dasso, Mary; Fontoura, Beatriz M A.

In: EMBO Reports, Vol. 10, No. 10, 14.09.2009, p. 1154-1160.

Research output: Contribution to journalArticle

Chakraborty, P, Seemann, J, Mishra, RK, Wei, JH, Weil, L, Nussenzveig, DR, Heiber, J, Barber, GN, Dasso, M & Fontoura, BMA 2009, 'Vesicular stomatitis virus inhibits mitotic progression and triggers cell death', EMBO Reports, vol. 10, no. 10, pp. 1154-1160. https://doi.org/10.1038/embor.2009.179
Chakraborty P, Seemann J, Mishra RK, Wei JH, Weil L, Nussenzveig DR et al. Vesicular stomatitis virus inhibits mitotic progression and triggers cell death. EMBO Reports. 2009 Sep 14;10(10):1154-1160. https://doi.org/10.1038/embor.2009.179
Chakraborty, Papia ; Seemann, Joachim ; Mishra, Ram K. ; Wei, Jen Hsuan ; Weil, Lauren ; Nussenzveig, Daniel R. ; Heiber, Joshua ; Barber, Glen N ; Dasso, Mary ; Fontoura, Beatriz M A. / Vesicular stomatitis virus inhibits mitotic progression and triggers cell death. In: EMBO Reports. 2009 ; Vol. 10, No. 10. pp. 1154-1160.
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