Very early and transient vegetal-plate expression of SpKrox1, a Kruppel/Krox gene from Strongylocentrotus purpuratus

Weiye Wang, Athula H. Wikramanayake, Maria Gonzalez-Rimbau, Antonia Vlahou, Constantin N. Flytzanis, William H. Klein

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

All endodermal and mesenchymal cells of the sea urchin embryo descend from the vegetal plate, a thickened epithelium of approximately 50 cells arising at the early blastula stage. Cell types that derive from the vegetal plate are specified conditionally by inductive interactions with underlying micromeres, but the molecular details of vegetal-plate specification remain unresolved. In a search for regulatory proteins that have roles in vegetal-plate specification, a screen was performed to clone Kruppel/Krox-related genes from a Strongylocentrotus purpuratus embryo cDNA library. One newly identified clone, named SpKrox1, contained four zinc fingers and a leucine zipper domain. SpKrox1 expression was low in unfertilized eggs, increased severalfold to the early blastula stage and decreased between the early gastrula and pluteus stages. SpKrox1 mRNA was first seen in macromeres of 16-cell stage embryos and was restricted to cells of the developing vegetal plate thereafter. Vegetal-plate expression corresponded to a ring of cells around the blastopore and overlapped the expression patterns of other genes with potential roles in vegetal plate-specification. As the vegetal-plate cells invaginated into the blastopore, SpKrox1 expression was lost, suggesting that its role was not in endoderm differentiation per se but rather in the initial establishment of the vegetal plate.

Original languageEnglish (US)
Pages (from-to)185-195
Number of pages11
JournalMechanisms of Development
Volume60
Issue number2
DOIs
StatePublished - Dec 1 1996
Externally publishedYes

Keywords

  • Kruppel/Krox genes
  • sea urchin embryos
  • vegetal-plate specification
  • zinc-finger transcription factors

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

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