Abstract
In cartilage tissue engineering the self-repair of articular cartilage requires monitoring of the cell's deposition of extracellular matrix, but current methods of observing destroy the sample. Sample destruction does not allow for longterm, continuous monitoring. The objective of the study was to test the bioreactor system ability to non-destructively measure properties of the tissue-engineered constructs. Porcine articular cartilage was decellularized and proteoglycans were removed. Porcine chondrocytes were seeded into the scaffolds using centrifugation within a cell seeding device and the scaffolds were put into culture plates for up to 21 days in culture medium. At different intervals, the scaffolds were analyzed using the bioreactor system, DMMB quantification, and histological techniques. The bioreactor measurements and DMMB quantification both confirmed there was a consistent increase in proteoglycan content within the scaffolds up to the 21 day endpoint, but the proteoglycan content of the constructs was only at 30% of the measured average content in native tissue. The bioreactor was able to measure the proteoglycan content accurately when compared to the measurements taken by DMMB quantification. The results of the study showed the bioreactor is useful for monitoring tissue growth in cartilage tissue engineering constructs non-destructively.
| Original language | English |
|---|---|
| Title of host publication | Proceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013 |
| Pages | 41-42 |
| Number of pages | 2 |
| DOIs | |
| State | Published - Aug 5 2013 |
| Event | 29th Southern Biomedical Engineering Conference, SBEC 2013 - Miami, FL, United States Duration: May 3 2013 → May 5 2013 |
Other
| Other | 29th Southern Biomedical Engineering Conference, SBEC 2013 |
|---|---|
| Country | United States |
| City | Miami, FL |
| Period | 5/3/13 → 5/5/13 |
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ASJC Scopus subject areas
- Biomedical Engineering
Cite this
Verification of measurements of cartilaginous tissue constructs in the online characterizing bioreactor system. / Echagarruga, Christina T.; Scanlon, Christopher M.; Yuan, Tai Yi; Huang, Chun-Yuh; Gu, Weiyong.
Proceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013. 2013. p. 41-42 6525666.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Verification of measurements of cartilaginous tissue constructs in the online characterizing bioreactor system
AU - Echagarruga, Christina T.
AU - Scanlon, Christopher M.
AU - Yuan, Tai Yi
AU - Huang, Chun-Yuh
AU - Gu, Weiyong
PY - 2013/8/5
Y1 - 2013/8/5
N2 - In cartilage tissue engineering the self-repair of articular cartilage requires monitoring of the cell's deposition of extracellular matrix, but current methods of observing destroy the sample. Sample destruction does not allow for longterm, continuous monitoring. The objective of the study was to test the bioreactor system ability to non-destructively measure properties of the tissue-engineered constructs. Porcine articular cartilage was decellularized and proteoglycans were removed. Porcine chondrocytes were seeded into the scaffolds using centrifugation within a cell seeding device and the scaffolds were put into culture plates for up to 21 days in culture medium. At different intervals, the scaffolds were analyzed using the bioreactor system, DMMB quantification, and histological techniques. The bioreactor measurements and DMMB quantification both confirmed there was a consistent increase in proteoglycan content within the scaffolds up to the 21 day endpoint, but the proteoglycan content of the constructs was only at 30% of the measured average content in native tissue. The bioreactor was able to measure the proteoglycan content accurately when compared to the measurements taken by DMMB quantification. The results of the study showed the bioreactor is useful for monitoring tissue growth in cartilage tissue engineering constructs non-destructively.
AB - In cartilage tissue engineering the self-repair of articular cartilage requires monitoring of the cell's deposition of extracellular matrix, but current methods of observing destroy the sample. Sample destruction does not allow for longterm, continuous monitoring. The objective of the study was to test the bioreactor system ability to non-destructively measure properties of the tissue-engineered constructs. Porcine articular cartilage was decellularized and proteoglycans were removed. Porcine chondrocytes were seeded into the scaffolds using centrifugation within a cell seeding device and the scaffolds were put into culture plates for up to 21 days in culture medium. At different intervals, the scaffolds were analyzed using the bioreactor system, DMMB quantification, and histological techniques. The bioreactor measurements and DMMB quantification both confirmed there was a consistent increase in proteoglycan content within the scaffolds up to the 21 day endpoint, but the proteoglycan content of the constructs was only at 30% of the measured average content in native tissue. The bioreactor was able to measure the proteoglycan content accurately when compared to the measurements taken by DMMB quantification. The results of the study showed the bioreactor is useful for monitoring tissue growth in cartilage tissue engineering constructs non-destructively.
UR - http://www.scopus.com/inward/record.url?scp=84880853313&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880853313&partnerID=8YFLogxK
U2 - 10.1109/SBEC.2013.29
DO - 10.1109/SBEC.2013.29
M3 - Conference contribution
AN - SCOPUS:84880853313
SN - 9780769550329
SP - 41
EP - 42
BT - Proceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013
ER -