Verification of measurements of cartilaginous tissue constructs in the online characterizing bioreactor system

Christina T. Echagarruga, Christopher M. Scanlon, Tai Yi Yuan, C. Y. Charles Huang, Wei Yong Gu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In cartilage tissue engineering the self-repair of articular cartilage requires monitoring of the cell's deposition of extracellular matrix, but current methods of observing destroy the sample. Sample destruction does not allow for longterm, continuous monitoring. The objective of the study was to test the bioreactor system ability to non-destructively measure properties of the tissue-engineered constructs. Porcine articular cartilage was decellularized and proteoglycans were removed. Porcine chondrocytes were seeded into the scaffolds using centrifugation within a cell seeding device and the scaffolds were put into culture plates for up to 21 days in culture medium. At different intervals, the scaffolds were analyzed using the bioreactor system, DMMB quantification, and histological techniques. The bioreactor measurements and DMMB quantification both confirmed there was a consistent increase in proteoglycan content within the scaffolds up to the 21 day endpoint, but the proteoglycan content of the constructs was only at 30% of the measured average content in native tissue. The bioreactor was able to measure the proteoglycan content accurately when compared to the measurements taken by DMMB quantification. The results of the study showed the bioreactor is useful for monitoring tissue growth in cartilage tissue engineering constructs non-destructively.

Original languageEnglish (US)
Title of host publicationProceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013
Pages41-42
Number of pages2
DOIs
StatePublished - Aug 5 2013
Event29th Southern Biomedical Engineering Conference, SBEC 2013 - Miami, FL, United States
Duration: May 3 2013May 5 2013

Publication series

NameProceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013

Other

Other29th Southern Biomedical Engineering Conference, SBEC 2013
CountryUnited States
CityMiami, FL
Period5/3/135/5/13

ASJC Scopus subject areas

  • Biomedical Engineering

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  • Cite this

    Echagarruga, C. T., Scanlon, C. M., Yuan, T. Y., Charles Huang, C. Y., & Gu, W. Y. (2013). Verification of measurements of cartilaginous tissue constructs in the online characterizing bioreactor system. In Proceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013 (pp. 41-42). [6525666] (Proceedings - 29th Southern Biomedical Engineering Conference, SBEC 2013). https://doi.org/10.1109/SBEC.2013.29