Verapamil has beneficial effects on ischemic myocardium, including reduction in electrophysiological derangements, prevention of intracellular K+ loss, and preservation of high-energy phosphates, but the mechanisms underlying these actions are not clear. Recent studies have demonstrated a role of ATP-regulated K+ (KATP) current in action potential shortening and K+ loss during ischemia and metabolic inhibition. Therefore, we studied the effects of verapamil on KATP current in feline ventricular myocytes to test the hypothesis that the drug prevents ischemic electrophysiological disturbances by affecting the KATP channel. Membrane potentials and currents were recorded using standard patch-clamp techniques. During 15-minute superfusion with 1 mM CN-, action potential duration measured at 90% repolarization was reduced from 259±12 to 98±15 msec (62% reduction) in the absence of verapamil and from 266±11 to 183±16 msec (31% reduction) in the presence of 2 μM verapamil (p<0.01). In inside-out membrane patches, the KATP current, activated in the absence of ATP, was significantly suppressed by intracellular application of 2 μM verapamil, but the single-channel conductance was not changed. Verapamil did not change the mean open and closed times of the channel within bursts (e.g., the mean open time was 1.92±0.18 and 1.82±0.21 msec in the absence and presence of 2 μM verapamil, respectively), but it shortened the mean lifetime of bursts from 41.1±3.5 to 24.9±2.8 msec (p<0.01) and prolonged the closed time between bursts from 39.4±4.6 to 78.5±5.1 msec (p<0.01). As a result, the open-state probability of the channel was significantly reduced from 0.31±0.04 to 0.03+0.01 (p<0.01). We suggest that these effects of verapamil on the KATP channel in isolated ventricular myocytes provide, in part, an explanation for its amelioration of electrophysiological disturbances and K+ loss during ischemia.
- Patch clamp
- Ventricular myocytes
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine