VEGFA links self-renewal and metastasis by inducing Sox2 to repress miR-452, driving Slug

M. Kim, K. Jang, P. Miller, M. Picon-Ruiz, T. M. Yeasky, Dorraya El-Ashry, Joyce M Slingerland

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Cancer stem cells (CSC) appear to have increased metastatic potential, but mechanisms underlying this are poorly defined. Here we show that VEGFA induction of Sox2 promotes EMT and tumor metastasis. In breast lines and primary cancer culture, VEGFA rapidly upregulates SOX2 expression, leading to SNAI2 induction, EMT, increased invasion and metastasis. We show Sox2 downregulates miR-452, which acts as a novel metastasis suppressor to directly target the SNAI2 3′-untranslated region (3′-UTR). VEGFA stimulates Sox2- and Slug-dependent cell invasion. VEGFA increases lung metastasis in vivo, and this is abrogated by miR-452 overexpression. Furthermore, SNAI2 transduction rescues metastasis suppression by miR-452. Thus, in addition to its angiogenic action, VEGFA upregulates Sox2 to drive stem cell expansion, together with miR-452 loss and Slug upregulation, providing a novel mechanism whereby cancer stem cells acquire metastatic potential. Prior work showed EMT transcription factor overexpression upregulates CSC. Present work indicates that stemness and metastasis are a two-way street: Sox2, a major mediator of CSC self-renewal, also governs the metastatic process.

Original languageEnglish (US)
Pages (from-to)5199-5211
Number of pages13
JournalOncogene
Volume36
Issue number36
DOIs
StatePublished - Sep 7 2017

Fingerprint

Gastropoda
Neoplastic Stem Cells
Neoplasm Metastasis
Up-Regulation
3' Untranslated Regions
Neoplasms
Breast
Transcription Factors
Stem Cells
Down-Regulation
Lung

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Kim, M., Jang, K., Miller, P., Picon-Ruiz, M., Yeasky, T. M., El-Ashry, D., & Slingerland, J. M. (2017). VEGFA links self-renewal and metastasis by inducing Sox2 to repress miR-452, driving Slug. Oncogene, 36(36), 5199-5211. https://doi.org/10.1038/onc.2017.4

VEGFA links self-renewal and metastasis by inducing Sox2 to repress miR-452, driving Slug. / Kim, M.; Jang, K.; Miller, P.; Picon-Ruiz, M.; Yeasky, T. M.; El-Ashry, Dorraya; Slingerland, Joyce M.

In: Oncogene, Vol. 36, No. 36, 07.09.2017, p. 5199-5211.

Research output: Contribution to journalArticle

Kim, M, Jang, K, Miller, P, Picon-Ruiz, M, Yeasky, TM, El-Ashry, D & Slingerland, JM 2017, 'VEGFA links self-renewal and metastasis by inducing Sox2 to repress miR-452, driving Slug', Oncogene, vol. 36, no. 36, pp. 5199-5211. https://doi.org/10.1038/onc.2017.4
Kim M, Jang K, Miller P, Picon-Ruiz M, Yeasky TM, El-Ashry D et al. VEGFA links self-renewal and metastasis by inducing Sox2 to repress miR-452, driving Slug. Oncogene. 2017 Sep 7;36(36):5199-5211. https://doi.org/10.1038/onc.2017.4
Kim, M. ; Jang, K. ; Miller, P. ; Picon-Ruiz, M. ; Yeasky, T. M. ; El-Ashry, Dorraya ; Slingerland, Joyce M. / VEGFA links self-renewal and metastasis by inducing Sox2 to repress miR-452, driving Slug. In: Oncogene. 2017 ; Vol. 36, No. 36. pp. 5199-5211.
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