VEGF increases retinal vascular ICAM-1 expression in vivo

Ming Lu, Victor L. Perez, Naili Ma, Kazuaki Miyamoto, Hai Bing Peng, James K. Liao, Anthony P. Adamis

Research output: Contribution to journalArticlepeer-review

204 Scopus citations

Abstract

Purpose. Intraocular injections of vascular endothelial growth factor (VEGF), a peptide implicated in the pathogenesis of diabetic retinopathy, can induce retinal ischemia. Diabetic retinal ischemia may be caused, in part, by the adhesion of leukocytes to the retinal vasculature. In this study, the ability of VEGF to increase the expression of intercellular adhesion molecule-1 (ICAM-1) and other adhesion molecules in capillary endothelium and the retinal vasculature was examined. Methods. The expression of ICAM-1, vascular cell adhesion molecule-1 (VCAM-1), E-selectin, and P-selectin on human brain capillary endothelial cell monolayers exposed to VEGF was quantitated by immunoassay. The effect of VEGF on retinal vascular ICAM-1 expression was determined in ICAM-1 immunofluorescence studies of retinal flat-mounts and in RNase protection assays. Results. VEGF increased capillary endothelial cell ICAM-1 levels in a dose- and time-dependent manner (6 -24 hours, plateau after 6 hours; EC50, 25 ng/ml). VEGF failed to alter E- selectin, P-selectin, or VCAM-1 levels under the conditions tested. Intravitreal injections of pathophysiologically relevant concentrations of VEGF increased ICAM-1 protein and mRNA levels in the retinal vasculature. Conclusions. VEGF increases retinal vascular ICAM-1 expression. VEGF-induced increases in ICAM-1 may promote retinal leukostasis in diabetic eyes.

Original languageEnglish (US)
Pages (from-to)1808-1812
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume40
Issue number8
StatePublished - Jul 8 1999

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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