VEGF increases retinal vascular ICAM-1 expression in vivo

Ming Lu, Victor L Perez Quinones, Naili Ma, Kazuaki Miyamoto, Hai Bing Peng, James K. Liao, Anthony P. Adamis

Research output: Contribution to journalArticle

200 Citations (Scopus)

Abstract

Purpose. Intraocular injections of vascular endothelial growth factor (VEGF), a peptide implicated in the pathogenesis of diabetic retinopathy, can induce retinal ischemia. Diabetic retinal ischemia may be caused, in part, by the adhesion of leukocytes to the retinal vasculature. In this study, the ability of VEGF to increase the expression of intercellular adhesion molecule-1 (ICAM-1) and other adhesion molecules in capillary endothelium and the retinal vasculature was examined. Methods. The expression of ICAM-1, vascular cell adhesion molecule-1 (VCAM-1), E-selectin, and P-selectin on human brain capillary endothelial cell monolayers exposed to VEGF was quantitated by immunoassay. The effect of VEGF on retinal vascular ICAM-1 expression was determined in ICAM-1 immunofluorescence studies of retinal flat-mounts and in RNase protection assays. Results. VEGF increased capillary endothelial cell ICAM-1 levels in a dose- and time-dependent manner (6 -24 hours, plateau after 6 hours; EC50, 25 ng/ml). VEGF failed to alter E- selectin, P-selectin, or VCAM-1 levels under the conditions tested. Intravitreal injections of pathophysiologically relevant concentrations of VEGF increased ICAM-1 protein and mRNA levels in the retinal vasculature. Conclusions. VEGF increases retinal vascular ICAM-1 expression. VEGF-induced increases in ICAM-1 may promote retinal leukostasis in diabetic eyes.

Original languageEnglish
Pages (from-to)1808-1812
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume40
Issue number8
StatePublished - Jul 8 1999
Externally publishedYes

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Retinal Vessels
Intercellular Adhesion Molecule-1
Vascular Endothelial Growth Factor A
E-Selectin
Vascular Cell Adhesion Molecule-1
Leukostasis
Ischemia
Endothelial Cells
Intraocular Injections
Intravitreal Injections
P-Selectin
Vascular Endothelium
Cell Adhesion Molecules
Diabetic Retinopathy
Ribonucleases
Immunoassay
Fluorescent Antibody Technique
Leukocytes
Messenger RNA
Peptides

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Lu, M., Perez Quinones, V. L., Ma, N., Miyamoto, K., Peng, H. B., Liao, J. K., & Adamis, A. P. (1999). VEGF increases retinal vascular ICAM-1 expression in vivo. Investigative Ophthalmology and Visual Science, 40(8), 1808-1812.

VEGF increases retinal vascular ICAM-1 expression in vivo. / Lu, Ming; Perez Quinones, Victor L; Ma, Naili; Miyamoto, Kazuaki; Peng, Hai Bing; Liao, James K.; Adamis, Anthony P.

In: Investigative Ophthalmology and Visual Science, Vol. 40, No. 8, 08.07.1999, p. 1808-1812.

Research output: Contribution to journalArticle

Lu, M, Perez Quinones, VL, Ma, N, Miyamoto, K, Peng, HB, Liao, JK & Adamis, AP 1999, 'VEGF increases retinal vascular ICAM-1 expression in vivo', Investigative Ophthalmology and Visual Science, vol. 40, no. 8, pp. 1808-1812.
Lu M, Perez Quinones VL, Ma N, Miyamoto K, Peng HB, Liao JK et al. VEGF increases retinal vascular ICAM-1 expression in vivo. Investigative Ophthalmology and Visual Science. 1999 Jul 8;40(8):1808-1812.
Lu, Ming ; Perez Quinones, Victor L ; Ma, Naili ; Miyamoto, Kazuaki ; Peng, Hai Bing ; Liao, James K. ; Adamis, Anthony P. / VEGF increases retinal vascular ICAM-1 expression in vivo. In: Investigative Ophthalmology and Visual Science. 1999 ; Vol. 40, No. 8. pp. 1808-1812.
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AU - Adamis, Anthony P.

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N2 - Purpose. Intraocular injections of vascular endothelial growth factor (VEGF), a peptide implicated in the pathogenesis of diabetic retinopathy, can induce retinal ischemia. Diabetic retinal ischemia may be caused, in part, by the adhesion of leukocytes to the retinal vasculature. In this study, the ability of VEGF to increase the expression of intercellular adhesion molecule-1 (ICAM-1) and other adhesion molecules in capillary endothelium and the retinal vasculature was examined. Methods. The expression of ICAM-1, vascular cell adhesion molecule-1 (VCAM-1), E-selectin, and P-selectin on human brain capillary endothelial cell monolayers exposed to VEGF was quantitated by immunoassay. The effect of VEGF on retinal vascular ICAM-1 expression was determined in ICAM-1 immunofluorescence studies of retinal flat-mounts and in RNase protection assays. Results. VEGF increased capillary endothelial cell ICAM-1 levels in a dose- and time-dependent manner (6 -24 hours, plateau after 6 hours; EC50, 25 ng/ml). VEGF failed to alter E- selectin, P-selectin, or VCAM-1 levels under the conditions tested. Intravitreal injections of pathophysiologically relevant concentrations of VEGF increased ICAM-1 protein and mRNA levels in the retinal vasculature. Conclusions. VEGF increases retinal vascular ICAM-1 expression. VEGF-induced increases in ICAM-1 may promote retinal leukostasis in diabetic eyes.

AB - Purpose. Intraocular injections of vascular endothelial growth factor (VEGF), a peptide implicated in the pathogenesis of diabetic retinopathy, can induce retinal ischemia. Diabetic retinal ischemia may be caused, in part, by the adhesion of leukocytes to the retinal vasculature. In this study, the ability of VEGF to increase the expression of intercellular adhesion molecule-1 (ICAM-1) and other adhesion molecules in capillary endothelium and the retinal vasculature was examined. Methods. The expression of ICAM-1, vascular cell adhesion molecule-1 (VCAM-1), E-selectin, and P-selectin on human brain capillary endothelial cell monolayers exposed to VEGF was quantitated by immunoassay. The effect of VEGF on retinal vascular ICAM-1 expression was determined in ICAM-1 immunofluorescence studies of retinal flat-mounts and in RNase protection assays. Results. VEGF increased capillary endothelial cell ICAM-1 levels in a dose- and time-dependent manner (6 -24 hours, plateau after 6 hours; EC50, 25 ng/ml). VEGF failed to alter E- selectin, P-selectin, or VCAM-1 levels under the conditions tested. Intravitreal injections of pathophysiologically relevant concentrations of VEGF increased ICAM-1 protein and mRNA levels in the retinal vasculature. Conclusions. VEGF increases retinal vascular ICAM-1 expression. VEGF-induced increases in ICAM-1 may promote retinal leukostasis in diabetic eyes.

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