Vector-mediated gene transfer engenders long-lived neutralizing activity and protection against SIV infection in monkeys

Philip R. Johnson, Bruce C. Schnepp, Jianchao Zhang, Mary J. Connell, Sean M. Greene, Eloisa Yuste, Ronald C. Desrosiers, K. Reed Clark

Research output: Contribution to journalArticle

235 Scopus citations

Abstract

The key to an effective HIV vaccine is development of an immunogen that elicits persisting antibodies with broad neutralizing activity against field strains of the virus. Unfortunately, very little progress has been made in finding or designing such immunogens. Using the simian immunodeficiency virus (SIV) model, we have taken a markedly different approach: delivery to muscle of an adeno-associated virus gene transfer vector expressing antibodies or antibody-like immunoadhesins having predetermined SIV specificity. With this approach, SIV-specific molecules are endogenously synthesized in myofibers and passively distributed to the circulatory system. Using such an approach in monkeys, we have now generated long-lasting neutralizing activity in serum and have observed complete protection against intravenous challenge with virulent SIV. In essence, this strategy bypasses the adaptive immune system and holds considerable promise as a unique approach to an effective HIV vaccine.

Original languageEnglish (US)
Pages (from-to)901-906
Number of pages6
JournalNature medicine
Volume15
Issue number8
DOIs
StatePublished - Aug 2009

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Vector-mediated gene transfer engenders long-lived neutralizing activity and protection against SIV infection in monkeys'. Together they form a unique fingerprint.

  • Cite this

    Johnson, P. R., Schnepp, B. C., Zhang, J., Connell, M. J., Greene, S. M., Yuste, E., Desrosiers, R. C., & Reed Clark, K. (2009). Vector-mediated gene transfer engenders long-lived neutralizing activity and protection against SIV infection in monkeys. Nature medicine, 15(8), 901-906. https://doi.org/10.1038/nm.1967