Abstract
Objective: To test the hypothesis that bcl-2 prevents oxidative stress-induced apoptosis of auditory sensory cells in explants of the organ of Corti and dissociated cell cultures of the spiral ganglion. Methods: Organ of Corti explants and dissociated spiral ganglion cell cultures obtained from 3-day-old (P3) rats or adult spiral ganglion cell cultures from 28-day-old (P28) rats were transduced with vectors containing a human bcl-2 gene. Cultures were then exposed to neomycin, cisplatin or subjected to withdrawal of neurotrophin supplementation. Outcome measures included hair cell and neuron counts, mitochondrial membrane potential and a histological measure of apoptosis. Results: Expression of bcl-2 in the organ of Corti explants and neuronal cell cultures provided a significant level of protection against cell death. Bcl-2 expression in the organ of Corti explants also protected mitochondria from loss of membrane potential and blocked an early step in the commitment of hair cells to apoptosis. Conclusion: Expression of bcl-2 in cochlear tissues protects sensory cells from a variety of insults that have been demonstrated to damage the inner ear.
| Original language | English |
|---|---|
| Pages (from-to) | 43-50 |
| Number of pages | 8 |
| Journal | ORL |
| Volume | 69 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 1 2006 |
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Keywords
- Antiapoptotic protein
- Auditory hair cells
- Auditory neurons
- Cell death
- Cochlea
- Otoprotection
- Ototoxins
- Oxidative stress
- Trophic support
ASJC Scopus subject areas
- Otorhinolaryngology
Cite this
Vector-mediated delivery of bcl-2 prevents degeneration of auditory hair cells and neurons after injury. / Staecker, Hinrich; Liu, Wei; Malgrange, Brigitte; Lefebvre, Philippe P.; Van De Water, Thomas R.
In: ORL, Vol. 69, No. 1, 01.12.2006, p. 43-50.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vector-mediated delivery of bcl-2 prevents degeneration of auditory hair cells and neurons after injury
AU - Staecker, Hinrich
AU - Liu, Wei
AU - Malgrange, Brigitte
AU - Lefebvre, Philippe P.
AU - Van De Water, Thomas R
PY - 2006/12/1
Y1 - 2006/12/1
N2 - Objective: To test the hypothesis that bcl-2 prevents oxidative stress-induced apoptosis of auditory sensory cells in explants of the organ of Corti and dissociated cell cultures of the spiral ganglion. Methods: Organ of Corti explants and dissociated spiral ganglion cell cultures obtained from 3-day-old (P3) rats or adult spiral ganglion cell cultures from 28-day-old (P28) rats were transduced with vectors containing a human bcl-2 gene. Cultures were then exposed to neomycin, cisplatin or subjected to withdrawal of neurotrophin supplementation. Outcome measures included hair cell and neuron counts, mitochondrial membrane potential and a histological measure of apoptosis. Results: Expression of bcl-2 in the organ of Corti explants and neuronal cell cultures provided a significant level of protection against cell death. Bcl-2 expression in the organ of Corti explants also protected mitochondria from loss of membrane potential and blocked an early step in the commitment of hair cells to apoptosis. Conclusion: Expression of bcl-2 in cochlear tissues protects sensory cells from a variety of insults that have been demonstrated to damage the inner ear.
AB - Objective: To test the hypothesis that bcl-2 prevents oxidative stress-induced apoptosis of auditory sensory cells in explants of the organ of Corti and dissociated cell cultures of the spiral ganglion. Methods: Organ of Corti explants and dissociated spiral ganglion cell cultures obtained from 3-day-old (P3) rats or adult spiral ganglion cell cultures from 28-day-old (P28) rats were transduced with vectors containing a human bcl-2 gene. Cultures were then exposed to neomycin, cisplatin or subjected to withdrawal of neurotrophin supplementation. Outcome measures included hair cell and neuron counts, mitochondrial membrane potential and a histological measure of apoptosis. Results: Expression of bcl-2 in the organ of Corti explants and neuronal cell cultures provided a significant level of protection against cell death. Bcl-2 expression in the organ of Corti explants also protected mitochondria from loss of membrane potential and blocked an early step in the commitment of hair cells to apoptosis. Conclusion: Expression of bcl-2 in cochlear tissues protects sensory cells from a variety of insults that have been demonstrated to damage the inner ear.
KW - Antiapoptotic protein
KW - Auditory hair cells
KW - Auditory neurons
KW - Cell death
KW - Cochlea
KW - Otoprotection
KW - Ototoxins
KW - Oxidative stress
KW - Trophic support
UR - http://www.scopus.com/inward/record.url?scp=33845804749&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845804749&partnerID=8YFLogxK
U2 - 10.1159/000096716
DO - 10.1159/000096716
M3 - Article
C2 - 17085952
AN - SCOPUS:33845804749
VL - 69
SP - 43
EP - 50
JO - ORL; journal for oto-rhino-laryngology and its related specialties
JF - ORL; journal for oto-rhino-laryngology and its related specialties
SN - 0301-1569
IS - 1
ER -