Vector-mediated delivery of bcl-2 prevents degeneration of auditory hair cells and neurons after injury

Hinrich Staecker, Wei Liu, Brigitte Malgrange, Philippe P. Lefebvre, Thomas R. Van De Water

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Objective: To test the hypothesis that bcl-2 prevents oxidative stress-induced apoptosis of auditory sensory cells in explants of the organ of Corti and dissociated cell cultures of the spiral ganglion. Methods: Organ of Corti explants and dissociated spiral ganglion cell cultures obtained from 3-day-old (P3) rats or adult spiral ganglion cell cultures from 28-day-old (P28) rats were transduced with vectors containing a human bcl-2 gene. Cultures were then exposed to neomycin, cisplatin or subjected to withdrawal of neurotrophin supplementation. Outcome measures included hair cell and neuron counts, mitochondrial membrane potential and a histological measure of apoptosis. Results: Expression of bcl-2 in the organ of Corti explants and neuronal cell cultures provided a significant level of protection against cell death. Bcl-2 expression in the organ of Corti explants also protected mitochondria from loss of membrane potential and blocked an early step in the commitment of hair cells to apoptosis. Conclusion: Expression of bcl-2 in cochlear tissues protects sensory cells from a variety of insults that have been demonstrated to damage the inner ear.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalORL
Volume69
Issue number1
DOIs
StatePublished - Dec 1 2006

Keywords

  • Antiapoptotic protein
  • Auditory hair cells
  • Auditory neurons
  • Cell death
  • Cochlea
  • Otoprotection
  • Ototoxins
  • Oxidative stress
  • Trophic support

ASJC Scopus subject areas

  • Otorhinolaryngology

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    Staecker, H., Liu, W., Malgrange, B., Lefebvre, P. P., & Van De Water, T. R. (2006). Vector-mediated delivery of bcl-2 prevents degeneration of auditory hair cells and neurons after injury. ORL, 69(1), 43-50. https://doi.org/10.1159/000096716