Vector-mediated delivery of bcl-2 prevents degeneration of auditory hair cells and neurons after injury

Hinrich Staecker, Wei Liu, Brigitte Malgrange, Philippe P. Lefebvre, Thomas R Van De Water

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Objective: To test the hypothesis that bcl-2 prevents oxidative stress-induced apoptosis of auditory sensory cells in explants of the organ of Corti and dissociated cell cultures of the spiral ganglion. Methods: Organ of Corti explants and dissociated spiral ganglion cell cultures obtained from 3-day-old (P3) rats or adult spiral ganglion cell cultures from 28-day-old (P28) rats were transduced with vectors containing a human bcl-2 gene. Cultures were then exposed to neomycin, cisplatin or subjected to withdrawal of neurotrophin supplementation. Outcome measures included hair cell and neuron counts, mitochondrial membrane potential and a histological measure of apoptosis. Results: Expression of bcl-2 in the organ of Corti explants and neuronal cell cultures provided a significant level of protection against cell death. Bcl-2 expression in the organ of Corti explants also protected mitochondria from loss of membrane potential and blocked an early step in the commitment of hair cells to apoptosis. Conclusion: Expression of bcl-2 in cochlear tissues protects sensory cells from a variety of insults that have been demonstrated to damage the inner ear.

Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalORL
Volume69
Issue number1
DOIs
StatePublished - Dec 1 2006

Fingerprint

Auditory Hair Cells
Organ of Corti
Spiral Ganglion
Cell Culture Techniques
Neurons
Wounds and Injuries
Apoptosis
bcl-2 Genes
Neomycin
Mitochondrial Membrane Potential
Cochlea
Nerve Growth Factors
Inner Ear
Membrane Potentials
Cisplatin
Mitochondria
Oxidative Stress
Cell Death
Cell Count
Outcome Assessment (Health Care)

Keywords

  • Antiapoptotic protein
  • Auditory hair cells
  • Auditory neurons
  • Cell death
  • Cochlea
  • Otoprotection
  • Ototoxins
  • Oxidative stress
  • Trophic support

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Staecker, H., Liu, W., Malgrange, B., Lefebvre, P. P., & Van De Water, T. R. (2006). Vector-mediated delivery of bcl-2 prevents degeneration of auditory hair cells and neurons after injury. ORL, 69(1), 43-50. https://doi.org/10.1159/000096716

Vector-mediated delivery of bcl-2 prevents degeneration of auditory hair cells and neurons after injury. / Staecker, Hinrich; Liu, Wei; Malgrange, Brigitte; Lefebvre, Philippe P.; Van De Water, Thomas R.

In: ORL, Vol. 69, No. 1, 01.12.2006, p. 43-50.

Research output: Contribution to journalArticle

Staecker, H, Liu, W, Malgrange, B, Lefebvre, PP & Van De Water, TR 2006, 'Vector-mediated delivery of bcl-2 prevents degeneration of auditory hair cells and neurons after injury', ORL, vol. 69, no. 1, pp. 43-50. https://doi.org/10.1159/000096716
Staecker, Hinrich ; Liu, Wei ; Malgrange, Brigitte ; Lefebvre, Philippe P. ; Van De Water, Thomas R. / Vector-mediated delivery of bcl-2 prevents degeneration of auditory hair cells and neurons after injury. In: ORL. 2006 ; Vol. 69, No. 1. pp. 43-50.
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