Vascular inflammation, insulin resistance, and endothelial dysfunction in salt-sensitive hypertension: Role of nuclear factor kappa B activation

Ming Sheng Zhou, Ivonne Hernandez Schulman, Leopoldo Raij

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Objectives: Activation of the nuclear factor kappa B (NFκB) inflammatory pathway by angiotensin II and reactive oxygen species may play an important role in the development of insulin resistance and cardiovascular injury in hypertensive and metabolic diseases. We have shown that in hypertensive Dahl salt-sensitive rats, upregulation of angiotensin II and reactive oxygen species contributed to increased vascular inflammatory gene expression, endothelial dysfunction, and insulin resistance. Herein, we investigated whether activation of NFκB contributes to the development of endothelial dysfunction, vascular injury, and vascular and peripheral insulin resistance in salt-sensitive hypertension. Methods: Dahl salt-sensitive rats were fed a normal (0.5% NaCl) or high-salt diet (4% NaCl), or high-salt diet and pyrrolidine dithiocarbamat (150 mg/kg in drinking water), an inhibitor of NFκB activation, for 6 weeks. Results: Hypertensive Dahl salt-sensitive rats manifested impaired endothelium-dependent relaxation to acetylcholine, aortic hypertrophy (35%), increased plasma C-reactive protein (25%), vascular superoxide (O2-) production (148%), and expression of monocyte chemoattractant protein-1, tumor necrosis factor alpha, phospho-IκBα, and phospho-(Ser536)-p65NFκB. Pyrrolidine dithiocarbamat significantly improved endothelium-dependent relaxation, reduced vascular O2-, and normalized aortic hypertrophy and systemic and local inflammation, despite only mildly reducing blood pressure. Hypertensive Dahl salt-sensitive rats also manifested impaired insulin-mediated vasorelaxation and Akt/endothelial nitric oxide synthase phosphorylation and decreased insulin sensitivity by hyperinsulinemic-euglycemic clamp (glucose infusion rate,-32%). Pyrrolidine dithiocarbamat significantly improved insulin-mediated vascular relaxation and Akt/endothelial nitric oxide synthase phosphorylation as well as insulin sensitivity. Conclusion: The current findings strongly suggest that activation of the NFκB inflammatory pathway by angiotensin II-induced reactive oxygen species generation may importantly contribute to vascular injury, systemic inflammation, as well as vascular and peripheral insulin resistance in salt-sensitive hypertension.

Original languageEnglish (US)
Pages (from-to)527-535
Number of pages9
JournalJournal of hypertension
Volume28
Issue number3
DOIs
StatePublished - Mar 2010

Keywords

  • Endothelial dysfunction
  • Hypertension
  • Insulin resistance
  • Nuclear factor kappa B
  • Vascular injury

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Vascular inflammation, insulin resistance, and endothelial dysfunction in salt-sensitive hypertension: Role of nuclear factor kappa B activation'. Together they form a unique fingerprint.

Cite this