Variation of peripapillary retinal nerve fiber layer birefringence in normal human subjects

XiangRun Huang, Harmobina Bagga, David Greenfield, Robert W. Knighton

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

PURPOSE: The retinal nerve fiber layer (RNFL) exhibits linear birefringence due to the oriented cylindrical structure of ganglion cell axons. The birefringence (Δn) depends on the density and composition of axonal organelles. The purpose of this study was to evaluate the distribution of birefringence around the optic nerve head (ONH) in normal subjects. METHODS. Birefringence was calculated along circular scan paths around the ONH as Δn = R/T, where R is RNFL retardance measured by scanning laser polarimetry (SLP) and T is RNFL thickness measured by optical coherence tomography (OCT). OCT scans on a 3.4 mm diameter circle were obtained from 26 normal subjects aged 18 to 53 years. Scans on circles with various diameters were obtained from 17 of these subjects. RESULTS. The average reproducibility of Δn measured on three separate days in four subjects was ±0.05 nm/μm. In most subjects Δn varied significantly along a circular path around the ONH, with maxima in superior and inferior bundles, minima temporally and nasally, and a mean of 0.32 ± 0.03 nm/μm. Δn profiles on circles of different diameter were similar, suggesting that Δn did not vary along nerve fiber bundles. CONCLUSIONS. RNFL birefringence varies with position around the ONH. This variation may result from known structural differences among nerve fiber bundles that serve different retinal regions. Constant Δn along bundles is consistent with this hypothesis. Measurements of RNFL birefringence may provide a means to detect early subcellular changes in glaucoma.

Original languageEnglish
Pages (from-to)3073-3080
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume45
Issue number9
DOIs
StatePublished - Sep 1 2004

Fingerprint

Birefringence
Nerve Fibers
Optic Disk
Optical Coherence Tomography
Scanning Laser Polarimetry
Ganglia
Glaucoma
Organelles
Axons

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Variation of peripapillary retinal nerve fiber layer birefringence in normal human subjects. / Huang, XiangRun; Bagga, Harmobina; Greenfield, David; Knighton, Robert W.

In: Investigative Ophthalmology and Visual Science, Vol. 45, No. 9, 01.09.2004, p. 3073-3080.

Research output: Contribution to journalArticle

@article{68c669797c84421ba8c6cd09f5122e24,
title = "Variation of peripapillary retinal nerve fiber layer birefringence in normal human subjects",
abstract = "PURPOSE: The retinal nerve fiber layer (RNFL) exhibits linear birefringence due to the oriented cylindrical structure of ganglion cell axons. The birefringence (Δn) depends on the density and composition of axonal organelles. The purpose of this study was to evaluate the distribution of birefringence around the optic nerve head (ONH) in normal subjects. METHODS. Birefringence was calculated along circular scan paths around the ONH as Δn = R/T, where R is RNFL retardance measured by scanning laser polarimetry (SLP) and T is RNFL thickness measured by optical coherence tomography (OCT). OCT scans on a 3.4 mm diameter circle were obtained from 26 normal subjects aged 18 to 53 years. Scans on circles with various diameters were obtained from 17 of these subjects. RESULTS. The average reproducibility of Δn measured on three separate days in four subjects was ±0.05 nm/μm. In most subjects Δn varied significantly along a circular path around the ONH, with maxima in superior and inferior bundles, minima temporally and nasally, and a mean of 0.32 ± 0.03 nm/μm. Δn profiles on circles of different diameter were similar, suggesting that Δn did not vary along nerve fiber bundles. CONCLUSIONS. RNFL birefringence varies with position around the ONH. This variation may result from known structural differences among nerve fiber bundles that serve different retinal regions. Constant Δn along bundles is consistent with this hypothesis. Measurements of RNFL birefringence may provide a means to detect early subcellular changes in glaucoma.",
author = "XiangRun Huang and Harmobina Bagga and David Greenfield and Knighton, {Robert W.}",
year = "2004",
month = "9",
day = "1",
doi = "10.1167/iovs.04-0110",
language = "English",
volume = "45",
pages = "3073--3080",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "9",

}

TY - JOUR

T1 - Variation of peripapillary retinal nerve fiber layer birefringence in normal human subjects

AU - Huang, XiangRun

AU - Bagga, Harmobina

AU - Greenfield, David

AU - Knighton, Robert W.

PY - 2004/9/1

Y1 - 2004/9/1

N2 - PURPOSE: The retinal nerve fiber layer (RNFL) exhibits linear birefringence due to the oriented cylindrical structure of ganglion cell axons. The birefringence (Δn) depends on the density and composition of axonal organelles. The purpose of this study was to evaluate the distribution of birefringence around the optic nerve head (ONH) in normal subjects. METHODS. Birefringence was calculated along circular scan paths around the ONH as Δn = R/T, where R is RNFL retardance measured by scanning laser polarimetry (SLP) and T is RNFL thickness measured by optical coherence tomography (OCT). OCT scans on a 3.4 mm diameter circle were obtained from 26 normal subjects aged 18 to 53 years. Scans on circles with various diameters were obtained from 17 of these subjects. RESULTS. The average reproducibility of Δn measured on three separate days in four subjects was ±0.05 nm/μm. In most subjects Δn varied significantly along a circular path around the ONH, with maxima in superior and inferior bundles, minima temporally and nasally, and a mean of 0.32 ± 0.03 nm/μm. Δn profiles on circles of different diameter were similar, suggesting that Δn did not vary along nerve fiber bundles. CONCLUSIONS. RNFL birefringence varies with position around the ONH. This variation may result from known structural differences among nerve fiber bundles that serve different retinal regions. Constant Δn along bundles is consistent with this hypothesis. Measurements of RNFL birefringence may provide a means to detect early subcellular changes in glaucoma.

AB - PURPOSE: The retinal nerve fiber layer (RNFL) exhibits linear birefringence due to the oriented cylindrical structure of ganglion cell axons. The birefringence (Δn) depends on the density and composition of axonal organelles. The purpose of this study was to evaluate the distribution of birefringence around the optic nerve head (ONH) in normal subjects. METHODS. Birefringence was calculated along circular scan paths around the ONH as Δn = R/T, where R is RNFL retardance measured by scanning laser polarimetry (SLP) and T is RNFL thickness measured by optical coherence tomography (OCT). OCT scans on a 3.4 mm diameter circle were obtained from 26 normal subjects aged 18 to 53 years. Scans on circles with various diameters were obtained from 17 of these subjects. RESULTS. The average reproducibility of Δn measured on three separate days in four subjects was ±0.05 nm/μm. In most subjects Δn varied significantly along a circular path around the ONH, with maxima in superior and inferior bundles, minima temporally and nasally, and a mean of 0.32 ± 0.03 nm/μm. Δn profiles on circles of different diameter were similar, suggesting that Δn did not vary along nerve fiber bundles. CONCLUSIONS. RNFL birefringence varies with position around the ONH. This variation may result from known structural differences among nerve fiber bundles that serve different retinal regions. Constant Δn along bundles is consistent with this hypothesis. Measurements of RNFL birefringence may provide a means to detect early subcellular changes in glaucoma.

UR - http://www.scopus.com/inward/record.url?scp=4344674192&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4344674192&partnerID=8YFLogxK

U2 - 10.1167/iovs.04-0110

DO - 10.1167/iovs.04-0110

M3 - Article

VL - 45

SP - 3073

EP - 3080

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 9

ER -