Variable effects of phosphorylation of Pit-1 dictated by the DNA response elements

Michael S. Kapiloff, Yigal Farkash, Michael Wegner, Michael G. Rosenfeld

Research output: Contribution to journalArticle

171 Scopus citations

Abstract

Pit-1, a tissue-specific POU domain transcription factor, is required for the activation of the prolactin, growth hormone, and Pit-1 promoters that confer regulation by epidermal growth factor, adenosine 3′,5′-monophosphate (cAMP), and phorbol esters. Pit-1 is phosphorylated in pituitary cells at two distinct sites in response to phorbol esters and cAMP. Phosphorylation of Pit-1 modifies its conformation on DNA recognition elements and results in increased binding at certain sites and decreased binding at other sites, dependent on DNA sequences adjacent to the core Pit-1 binding motif. One residue (Thr220), located in the POU homeodomain within a sequence conserved throughout the POU-domain family, confers these responses.

Original languageEnglish (US)
Pages (from-to)786-789
Number of pages4
JournalScience
Volume253
Issue number5021
DOIs
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • General

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    Kapiloff, M. S., Farkash, Y., Wegner, M., & Rosenfeld, M. G. (1991). Variable effects of phosphorylation of Pit-1 dictated by the DNA response elements. Science, 253(5021), 786-789. https://doi.org/10.1126/science.1652153