Abstract
The Goldman-Hodgkin-Katz equation has been extensively used to determine cationic/anionic permeability ratios in the paracellular pathways of the gallbladder epithelium. Nevertheless, new experimental evidence suggests that none of the theoretical assumptions of the equation hold for these pathways. In order to assess the experimental validity of the Goldman equation the permeability ratios were calculated from zero-current diffusion potentials by means of the Goldman equation and compared with the cationic/anionic permeability ratios measured by simultaneous determinations of cation and anion tracer fluxes in the same membranes. The results indicate that the Goldman equation is empirically valid for the tested salts (KCl and RbCl) within the experimental range of concentrations (25 to 200 mM) at an electrochemical-potential difference of zero.
| Original language | English |
|---|---|
| Pages (from-to) | 178-182 |
| Number of pages | 5 |
| Journal | BBA - Biomembranes |
| Volume | 691 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 24 1982 |
| Externally published | Yes |
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Keywords
- (Toad gallbladder)
- Cation channel
- Epithelial membrane
- Goldman equation
- Paracellular ionic pathway
- Permeability ratio
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Medicine(all)
Cite this
Validity of the Goldman-Hodgkin-Katz equation in paracellular ionic pathways of gallbladder epithelium. / Salas, Pedro J; López, Ester M.
In: BBA - Biomembranes, Vol. 691, No. 1, 24.09.1982, p. 178-182.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Validity of the Goldman-Hodgkin-Katz equation in paracellular ionic pathways of gallbladder epithelium
AU - Salas, Pedro J
AU - López, Ester M.
PY - 1982/9/24
Y1 - 1982/9/24
N2 - The Goldman-Hodgkin-Katz equation has been extensively used to determine cationic/anionic permeability ratios in the paracellular pathways of the gallbladder epithelium. Nevertheless, new experimental evidence suggests that none of the theoretical assumptions of the equation hold for these pathways. In order to assess the experimental validity of the Goldman equation the permeability ratios were calculated from zero-current diffusion potentials by means of the Goldman equation and compared with the cationic/anionic permeability ratios measured by simultaneous determinations of cation and anion tracer fluxes in the same membranes. The results indicate that the Goldman equation is empirically valid for the tested salts (KCl and RbCl) within the experimental range of concentrations (25 to 200 mM) at an electrochemical-potential difference of zero.
AB - The Goldman-Hodgkin-Katz equation has been extensively used to determine cationic/anionic permeability ratios in the paracellular pathways of the gallbladder epithelium. Nevertheless, new experimental evidence suggests that none of the theoretical assumptions of the equation hold for these pathways. In order to assess the experimental validity of the Goldman equation the permeability ratios were calculated from zero-current diffusion potentials by means of the Goldman equation and compared with the cationic/anionic permeability ratios measured by simultaneous determinations of cation and anion tracer fluxes in the same membranes. The results indicate that the Goldman equation is empirically valid for the tested salts (KCl and RbCl) within the experimental range of concentrations (25 to 200 mM) at an electrochemical-potential difference of zero.
KW - (Toad gallbladder)
KW - Cation channel
KW - Epithelial membrane
KW - Goldman equation
KW - Paracellular ionic pathway
KW - Permeability ratio
UR - http://www.scopus.com/inward/record.url?scp=0020423894&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020423894&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(82)90227-9
DO - 10.1016/0005-2736(82)90227-9
M3 - Article
C2 - 6291606
AN - SCOPUS:0020423894
VL - 691
SP - 178
EP - 182
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 1
ER -