Vagotomy ameliorates islet morphofunction and body metabolic homeostasis in MSG-obese rats

C. Lubaczeuski, S. L. Balbo, R. A. Ribeiro, J. F. Vettorazzi, J. C. Santos-Silva, E. M. Carneiro, M. L. Bonfleur

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The parasympathetic nervous system is important for b-cell secretion and mass regulation. Here, we characterized involvement of the vagus nerve in pancreatic b-cell morphofunctional regulation and body nutrient homeostasis in 90-day-old monosodium glutamate (MSG)-obese rats. Male newborn Wistar rats received MSG (4 g/kg body weight) or saline [control (CTL) group] during the first 5 days of life. At 30 days of age, both groups of rats were submitted to sham-surgery (CTL and MSG groups) or subdiaphragmatic vagotomy (Cvag and Mvag groups). The 90-day-old MSG rats presented obesity, hyperinsulinemia, insulin resistance, and hypertriglyceridemia. Their pancreatic islets hypersecreted insulin in response to glucose but did not increase insulin release upon carbachol (Cch) stimulus, despite a higher intracellular Ca2+ mobilization. Furthermore, while the pancreas weight was 34% lower in MSG rats, no alteration in islet and b-cell mass was observed. However, in the MSG pancreas, increases of 51% and 55% were observed in the total islet and b-cell area/pancreas section, respectively. Also, the b-cell number per b-cell area was 19% higher in MSG rat pancreas than in CTL pancreas. Vagotomy prevented obesity, reducing 25% of body fat stores and ameliorated glucose homeostasis in Mvag rats. Mvag islets demonstrated partially reduced insulin secretion in response to 11.1 mM glucose and presented normalization of Cch-induced Ca2+ mobilization and insulin release. All morphometric parameters were similar among Mvag and CTL rat pancreases. Therefore, the higher insulin release in MSG rats was associated with greater b-cell/islet numbers and not due to hypertrophy. Vagotomy improved whole body nutrient homeostasis and endocrine pancreatic morphofunction in Mvag rats.

Original languageEnglish (US)
Pages (from-to)447-457
Number of pages11
JournalBrazilian Journal of Medical and Biological Research
Volume48
Issue number5
DOIs
StatePublished - 2015
Externally publishedYes

Keywords

  • Obesity
  • Parasympathetic nervous system
  • β-Cell

ASJC Scopus subject areas

  • Biophysics
  • Neuroscience(all)
  • Biochemistry
  • Physiology
  • Immunology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Cell Biology

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