Vaccine-induced cellular immune responses reduce plasma viral concentrations after repeated low-dose challenge with pathogenic simian immunodeficiency virus SIVmac239

Nancy A. Wilson, Jason Reed, Gnankang S. Napoe, Shari Piaskowski, Andy Szymanski, Jessica Furlott, Edna J. Gonzalez, Levi J. Yant, Nicholas J. Maness, Gemma E. May, Taeko Soma, Matthew R. Reynolds, Eva Rakasz, Richard Rudersdorf, Adrian B. McDermott, David H. O'Connor, Thomas C. Friedrich, David B. Allison, Amit Patki, Louis J. PickerDennis R. Burton, Jing Lin, Lingyi Huang, Deepa Patel, Gwendolyn Heindecker, Jiang Fan, Michael Citron, Melanie Horton, Fubao Wang, Xiaoping Liang, John W. Shiver, Danilo R. Casimiro, David Watkins

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

The goal of an AIDS vaccine regimen designed to induce cellular immune responses should be to reduce the viral set point and preserve memory CD4 lymphocytes. Here we investigated whether vaccine-induced cellular immunity in the absence of any Env-specific antibodies can control viral replication following multiple low-dose challenges with the highly pathogenic SIVmac239 isolate. Eight Mamu-A*01-positive Indian rhesus macaques were vaccinated with simian immunodeficiency virus (SIV) gag, tat, rev, and nef using a DNA prime-adenovirus boost strategy. Peak viremia (P = 0.007) and the chronic phase set point (P = 0.0192) were significantly decreased in the vaccinated cohort, out to 1 year postinfection. Loss of CD4+ memory populations was also ameliorated in vaccinated animals. Interestingly, only one of the eight vaccinees developed Env-specific neutralizing antibodies after infection. The control observed was significantly improved over that observed in animals vaccinated with SIV gag only. Vaccine-induced cellular immune responses can, therefore, exert a measure of control over replication of the AIDS virus in the complete absence of neutralizing antibody and give us hope that a vaccine designed to induce cellular immune responses might control viral replication.

Original languageEnglish
Pages (from-to)5875-5885
Number of pages11
JournalJournal of Virology
Volume80
Issue number12
DOIs
StatePublished - Jun 1 2006
Externally publishedYes

Fingerprint

Simian immunodeficiency virus
Simian Immunodeficiency Virus
Cellular Immunity
cell-mediated immunity
Vaccines
vaccines
virus replication
dosage
Neutralizing Antibodies
neutralizing antibodies
Hope
AIDS Vaccines
Viremia
viremia
Memory Disorders
Adenoviridae
Macaca mulatta
preserves
control methods
animals

ASJC Scopus subject areas

  • Immunology

Cite this

Vaccine-induced cellular immune responses reduce plasma viral concentrations after repeated low-dose challenge with pathogenic simian immunodeficiency virus SIVmac239. / Wilson, Nancy A.; Reed, Jason; Napoe, Gnankang S.; Piaskowski, Shari; Szymanski, Andy; Furlott, Jessica; Gonzalez, Edna J.; Yant, Levi J.; Maness, Nicholas J.; May, Gemma E.; Soma, Taeko; Reynolds, Matthew R.; Rakasz, Eva; Rudersdorf, Richard; McDermott, Adrian B.; O'Connor, David H.; Friedrich, Thomas C.; Allison, David B.; Patki, Amit; Picker, Louis J.; Burton, Dennis R.; Lin, Jing; Huang, Lingyi; Patel, Deepa; Heindecker, Gwendolyn; Fan, Jiang; Citron, Michael; Horton, Melanie; Wang, Fubao; Liang, Xiaoping; Shiver, John W.; Casimiro, Danilo R.; Watkins, David.

In: Journal of Virology, Vol. 80, No. 12, 01.06.2006, p. 5875-5885.

Research output: Contribution to journalArticle

Wilson, NA, Reed, J, Napoe, GS, Piaskowski, S, Szymanski, A, Furlott, J, Gonzalez, EJ, Yant, LJ, Maness, NJ, May, GE, Soma, T, Reynolds, MR, Rakasz, E, Rudersdorf, R, McDermott, AB, O'Connor, DH, Friedrich, TC, Allison, DB, Patki, A, Picker, LJ, Burton, DR, Lin, J, Huang, L, Patel, D, Heindecker, G, Fan, J, Citron, M, Horton, M, Wang, F, Liang, X, Shiver, JW, Casimiro, DR & Watkins, D 2006, 'Vaccine-induced cellular immune responses reduce plasma viral concentrations after repeated low-dose challenge with pathogenic simian immunodeficiency virus SIVmac239', Journal of Virology, vol. 80, no. 12, pp. 5875-5885. https://doi.org/10.1128/JVI.00171-06
Wilson, Nancy A. ; Reed, Jason ; Napoe, Gnankang S. ; Piaskowski, Shari ; Szymanski, Andy ; Furlott, Jessica ; Gonzalez, Edna J. ; Yant, Levi J. ; Maness, Nicholas J. ; May, Gemma E. ; Soma, Taeko ; Reynolds, Matthew R. ; Rakasz, Eva ; Rudersdorf, Richard ; McDermott, Adrian B. ; O'Connor, David H. ; Friedrich, Thomas C. ; Allison, David B. ; Patki, Amit ; Picker, Louis J. ; Burton, Dennis R. ; Lin, Jing ; Huang, Lingyi ; Patel, Deepa ; Heindecker, Gwendolyn ; Fan, Jiang ; Citron, Michael ; Horton, Melanie ; Wang, Fubao ; Liang, Xiaoping ; Shiver, John W. ; Casimiro, Danilo R. ; Watkins, David. / Vaccine-induced cellular immune responses reduce plasma viral concentrations after repeated low-dose challenge with pathogenic simian immunodeficiency virus SIVmac239. In: Journal of Virology. 2006 ; Vol. 80, No. 12. pp. 5875-5885.
@article{9ff51c35065c47cab1f32b03e168ca5a,
title = "Vaccine-induced cellular immune responses reduce plasma viral concentrations after repeated low-dose challenge with pathogenic simian immunodeficiency virus SIVmac239",
abstract = "The goal of an AIDS vaccine regimen designed to induce cellular immune responses should be to reduce the viral set point and preserve memory CD4 lymphocytes. Here we investigated whether vaccine-induced cellular immunity in the absence of any Env-specific antibodies can control viral replication following multiple low-dose challenges with the highly pathogenic SIVmac239 isolate. Eight Mamu-A*01-positive Indian rhesus macaques were vaccinated with simian immunodeficiency virus (SIV) gag, tat, rev, and nef using a DNA prime-adenovirus boost strategy. Peak viremia (P = 0.007) and the chronic phase set point (P = 0.0192) were significantly decreased in the vaccinated cohort, out to 1 year postinfection. Loss of CD4+ memory populations was also ameliorated in vaccinated animals. Interestingly, only one of the eight vaccinees developed Env-specific neutralizing antibodies after infection. The control observed was significantly improved over that observed in animals vaccinated with SIV gag only. Vaccine-induced cellular immune responses can, therefore, exert a measure of control over replication of the AIDS virus in the complete absence of neutralizing antibody and give us hope that a vaccine designed to induce cellular immune responses might control viral replication.",
author = "Wilson, {Nancy A.} and Jason Reed and Napoe, {Gnankang S.} and Shari Piaskowski and Andy Szymanski and Jessica Furlott and Gonzalez, {Edna J.} and Yant, {Levi J.} and Maness, {Nicholas J.} and May, {Gemma E.} and Taeko Soma and Reynolds, {Matthew R.} and Eva Rakasz and Richard Rudersdorf and McDermott, {Adrian B.} and O'Connor, {David H.} and Friedrich, {Thomas C.} and Allison, {David B.} and Amit Patki and Picker, {Louis J.} and Burton, {Dennis R.} and Jing Lin and Lingyi Huang and Deepa Patel and Gwendolyn Heindecker and Jiang Fan and Michael Citron and Melanie Horton and Fubao Wang and Xiaoping Liang and Shiver, {John W.} and Casimiro, {Danilo R.} and David Watkins",
year = "2006",
month = "6",
day = "1",
doi = "10.1128/JVI.00171-06",
language = "English",
volume = "80",
pages = "5875--5885",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - Vaccine-induced cellular immune responses reduce plasma viral concentrations after repeated low-dose challenge with pathogenic simian immunodeficiency virus SIVmac239

AU - Wilson, Nancy A.

AU - Reed, Jason

AU - Napoe, Gnankang S.

AU - Piaskowski, Shari

AU - Szymanski, Andy

AU - Furlott, Jessica

AU - Gonzalez, Edna J.

AU - Yant, Levi J.

AU - Maness, Nicholas J.

AU - May, Gemma E.

AU - Soma, Taeko

AU - Reynolds, Matthew R.

AU - Rakasz, Eva

AU - Rudersdorf, Richard

AU - McDermott, Adrian B.

AU - O'Connor, David H.

AU - Friedrich, Thomas C.

AU - Allison, David B.

AU - Patki, Amit

AU - Picker, Louis J.

AU - Burton, Dennis R.

AU - Lin, Jing

AU - Huang, Lingyi

AU - Patel, Deepa

AU - Heindecker, Gwendolyn

AU - Fan, Jiang

AU - Citron, Michael

AU - Horton, Melanie

AU - Wang, Fubao

AU - Liang, Xiaoping

AU - Shiver, John W.

AU - Casimiro, Danilo R.

AU - Watkins, David

PY - 2006/6/1

Y1 - 2006/6/1

N2 - The goal of an AIDS vaccine regimen designed to induce cellular immune responses should be to reduce the viral set point and preserve memory CD4 lymphocytes. Here we investigated whether vaccine-induced cellular immunity in the absence of any Env-specific antibodies can control viral replication following multiple low-dose challenges with the highly pathogenic SIVmac239 isolate. Eight Mamu-A*01-positive Indian rhesus macaques were vaccinated with simian immunodeficiency virus (SIV) gag, tat, rev, and nef using a DNA prime-adenovirus boost strategy. Peak viremia (P = 0.007) and the chronic phase set point (P = 0.0192) were significantly decreased in the vaccinated cohort, out to 1 year postinfection. Loss of CD4+ memory populations was also ameliorated in vaccinated animals. Interestingly, only one of the eight vaccinees developed Env-specific neutralizing antibodies after infection. The control observed was significantly improved over that observed in animals vaccinated with SIV gag only. Vaccine-induced cellular immune responses can, therefore, exert a measure of control over replication of the AIDS virus in the complete absence of neutralizing antibody and give us hope that a vaccine designed to induce cellular immune responses might control viral replication.

AB - The goal of an AIDS vaccine regimen designed to induce cellular immune responses should be to reduce the viral set point and preserve memory CD4 lymphocytes. Here we investigated whether vaccine-induced cellular immunity in the absence of any Env-specific antibodies can control viral replication following multiple low-dose challenges with the highly pathogenic SIVmac239 isolate. Eight Mamu-A*01-positive Indian rhesus macaques were vaccinated with simian immunodeficiency virus (SIV) gag, tat, rev, and nef using a DNA prime-adenovirus boost strategy. Peak viremia (P = 0.007) and the chronic phase set point (P = 0.0192) were significantly decreased in the vaccinated cohort, out to 1 year postinfection. Loss of CD4+ memory populations was also ameliorated in vaccinated animals. Interestingly, only one of the eight vaccinees developed Env-specific neutralizing antibodies after infection. The control observed was significantly improved over that observed in animals vaccinated with SIV gag only. Vaccine-induced cellular immune responses can, therefore, exert a measure of control over replication of the AIDS virus in the complete absence of neutralizing antibody and give us hope that a vaccine designed to induce cellular immune responses might control viral replication.

UR - http://www.scopus.com/inward/record.url?scp=33744902339&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744902339&partnerID=8YFLogxK

U2 - 10.1128/JVI.00171-06

DO - 10.1128/JVI.00171-06

M3 - Article

C2 - 16731926

AN - SCOPUS:33744902339

VL - 80

SP - 5875

EP - 5885

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 12

ER -