Uveal Melanoma Cell Lines: Where do they come from? (An American Ophthalmological Society Thesis)

Martine J. Jager, J. Antonio Bermudez Magner, Bruce R. Ksander, Sander Dubovy

Research output: Contribution to journalReview article

6 Citations (Scopus)

Abstract

PURPOSE: To determine whether some of the most often used uveal melanoma cell lines resemble their original tumor.

METHODS: Analysis of the literature, patient charts, histopathology, mutations, chromosome status, HLA type, and expression of melanocyte markers on cell lines and their primary tumors. We examined five cell lines and the primary tumors from which they were derived.

RESULTS: Four of the five examined primary tumors were unusual: one occupied the orbit, two were recurrences after prior irradiation, and one developed in an eye with a nevus of Ota. One cell line did not contain the GNA11 mutation, but it was present in the primary tumor. Three of the primary tumors had monosomy 3 (two of these lacked BAP1 expression); however, all five cell lines showed disomy 3 and BAP1 expression. All of the cell lines had gain of 8q. Two cell lines lacked expression of melanocyte markers, although these were present in the corresponding primary tumor.

CONCLUSIONS: All cell lines could be traced back to their original uveal melanoma. Four of the five primary tumors were unusual. Cell lines often differed from their primary tumor in chromosome status and melanocyte markers. However, their specific chromosome aberrations and capacity to continue proliferation characterize them as uveal melanoma cell lines.

Original languageEnglish (US)
Pages (from-to)T5
JournalTransactions of the American Ophthalmological Society
Volume114
StatePublished - Aug 1 2016

Fingerprint

Cell Line
Melanocytes
Neoplasms
Chromosomes
Uveal melanoma
Monosomy
Mutation
Nevus
Orbit
Tumor Cell Line
Chromosome Aberrations
Recurrence

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Uveal Melanoma Cell Lines : Where do they come from? (An American Ophthalmological Society Thesis). / Jager, Martine J.; Magner, J. Antonio Bermudez; Ksander, Bruce R.; Dubovy, Sander.

In: Transactions of the American Ophthalmological Society, Vol. 114, 01.08.2016, p. T5.

Research output: Contribution to journalReview article

@article{2cc3441403e0471988f06ca2f7651de9,
title = "Uveal Melanoma Cell Lines: Where do they come from? (An American Ophthalmological Society Thesis)",
abstract = "PURPOSE: To determine whether some of the most often used uveal melanoma cell lines resemble their original tumor.METHODS: Analysis of the literature, patient charts, histopathology, mutations, chromosome status, HLA type, and expression of melanocyte markers on cell lines and their primary tumors. We examined five cell lines and the primary tumors from which they were derived.RESULTS: Four of the five examined primary tumors were unusual: one occupied the orbit, two were recurrences after prior irradiation, and one developed in an eye with a nevus of Ota. One cell line did not contain the GNA11 mutation, but it was present in the primary tumor. Three of the primary tumors had monosomy 3 (two of these lacked BAP1 expression); however, all five cell lines showed disomy 3 and BAP1 expression. All of the cell lines had gain of 8q. Two cell lines lacked expression of melanocyte markers, although these were present in the corresponding primary tumor.CONCLUSIONS: All cell lines could be traced back to their original uveal melanoma. Four of the five primary tumors were unusual. Cell lines often differed from their primary tumor in chromosome status and melanocyte markers. However, their specific chromosome aberrations and capacity to continue proliferation characterize them as uveal melanoma cell lines.",
author = "Jager, {Martine J.} and Magner, {J. Antonio Bermudez} and Ksander, {Bruce R.} and Sander Dubovy",
year = "2016",
month = "8",
day = "1",
language = "English (US)",
volume = "114",
pages = "T5",
journal = "Transactions of the American Ophthalmological Society",
issn = "0065-9533",
publisher = "American Ophthalmological Society",

}

TY - JOUR

T1 - Uveal Melanoma Cell Lines

T2 - Where do they come from? (An American Ophthalmological Society Thesis)

AU - Jager, Martine J.

AU - Magner, J. Antonio Bermudez

AU - Ksander, Bruce R.

AU - Dubovy, Sander

PY - 2016/8/1

Y1 - 2016/8/1

N2 - PURPOSE: To determine whether some of the most often used uveal melanoma cell lines resemble their original tumor.METHODS: Analysis of the literature, patient charts, histopathology, mutations, chromosome status, HLA type, and expression of melanocyte markers on cell lines and their primary tumors. We examined five cell lines and the primary tumors from which they were derived.RESULTS: Four of the five examined primary tumors were unusual: one occupied the orbit, two were recurrences after prior irradiation, and one developed in an eye with a nevus of Ota. One cell line did not contain the GNA11 mutation, but it was present in the primary tumor. Three of the primary tumors had monosomy 3 (two of these lacked BAP1 expression); however, all five cell lines showed disomy 3 and BAP1 expression. All of the cell lines had gain of 8q. Two cell lines lacked expression of melanocyte markers, although these were present in the corresponding primary tumor.CONCLUSIONS: All cell lines could be traced back to their original uveal melanoma. Four of the five primary tumors were unusual. Cell lines often differed from their primary tumor in chromosome status and melanocyte markers. However, their specific chromosome aberrations and capacity to continue proliferation characterize them as uveal melanoma cell lines.

AB - PURPOSE: To determine whether some of the most often used uveal melanoma cell lines resemble their original tumor.METHODS: Analysis of the literature, patient charts, histopathology, mutations, chromosome status, HLA type, and expression of melanocyte markers on cell lines and their primary tumors. We examined five cell lines and the primary tumors from which they were derived.RESULTS: Four of the five examined primary tumors were unusual: one occupied the orbit, two were recurrences after prior irradiation, and one developed in an eye with a nevus of Ota. One cell line did not contain the GNA11 mutation, but it was present in the primary tumor. Three of the primary tumors had monosomy 3 (two of these lacked BAP1 expression); however, all five cell lines showed disomy 3 and BAP1 expression. All of the cell lines had gain of 8q. Two cell lines lacked expression of melanocyte markers, although these were present in the corresponding primary tumor.CONCLUSIONS: All cell lines could be traced back to their original uveal melanoma. Four of the five primary tumors were unusual. Cell lines often differed from their primary tumor in chromosome status and melanocyte markers. However, their specific chromosome aberrations and capacity to continue proliferation characterize them as uveal melanoma cell lines.

UR - http://www.scopus.com/inward/record.url?scp=85047593531&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047593531&partnerID=8YFLogxK

M3 - Review article

C2 - 28018010

AN - SCOPUS:85047593531

VL - 114

SP - T5

JO - Transactions of the American Ophthalmological Society

JF - Transactions of the American Ophthalmological Society

SN - 0065-9533

ER -