UV photoprotection by combination topical antioxidants vitamin C and vitamin E

Jing Yi Lin, M. Angelica Selim, Christopher R. Shea, James M. Grichnik, Mostafa M. Omar, Nancy A. Monteiro-Riviere, Sheldon R. Pinnell

Research output: Contribution to journalArticlepeer-review

236 Scopus citations


Background: Virtually all plants and animals protect themselves from the sun using vitamins C and E. Objective: The purpose of this study was to see if a combination of topical vitamins C and E is better for UV protection to skin than an equivalent concentration of topical vitamin C or E alone. Methods: We developed a stable aqueous solution of 15% L-ascorbic acid (vitamin C) and 1% α-tocopherol (vitamin E). We applied antioxidant or vehicle solutions to pig skin daily for 4 days. We irradiated (1-5 × minimal erythema dose) control- and antioxidant-treated skin using a solar simulator with a 295-nm band-pass filter. On day 5, we measured antioxidant protection factor, erythema, sunburn cells, and thymine dimers. Results: The combination of 15% L-ascorbic acid and 1% α-tocopherol provided significant protection against erythema and sunburn cell formation; either L-ascorbic acid or 1% α-tocopherol alone also was protective but the combination was superior. Application during 4 days provided progressive protection that yielded an antioxidant protection factor of 4-fold. In addition, the combination of vitamins C and E provided protection against thymine dimer formation. Conclusion: Appreciable photoprotection can be obtained from the combination of topical vitamins C and E. We suggest that these natural products may protect against skin cancer and photoaging.

Original languageEnglish (US)
Pages (from-to)866-874
Number of pages9
JournalJournal of the American Academy of Dermatology
Issue number6
StatePublished - Jun 1 2003

ASJC Scopus subject areas

  • Dermatology


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