Utilization of dried blood spot citrulline level as a noninvasive method for monitoring graft function following intestinal transplantation

Chul Yu Hee, Sony Tuteja, Il Moon Jang, Gary I. Kleiner, Lobella Conanan, Jeffrey J. Gaynor, Tomoaki Kato, David M. Levi, Seigo Nishida, Gennaro Selvaggi, Carlos Gandia, Debbie Weppler, Violet Esquenazi, Phillip Ruiz, Joshua Miller, Andreas G. Tzakis

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33 Scopus citations

Abstract

Background. Citrulline concentrations have been proposed as a marker for intestinal allograft rejection. We instituted dried blood spot (DBS) specimen monitoring of citrulline to simplify sample collection posttransplant. This study demonstrates the correlation between plasma and dried blood spot specimen citrulline concentrations after intestinal transplantation. Methods. Plasma and DBS samples were analyzed by hydrophilic interaction chromatography tandem mass spectrometry. Comparison of the strength of linear correlation was made according to the type of surgery, sonication time, DBS citrulline levels, and the time interval between the blood sample collection and the assay date. Results. A very strong linear correlation exists between the plasma and DBS citrulline concentrations (r2=0.87; P<0.001). The correlation between plasma and DBS citrulline concentrations was maintained when evaluating only the intestinal transplant recipients. There was no significant difference in the strength of linear correlation according to sonication time, cirtrulline concentrations, or length of time to assay date. Conclusions. DBS citrulline monitoring will ease sample collection following intestinal transplantation and improve the ability to detect intestinal dysfunction and rejection by a noninvasive means.

Original languageEnglish (US)
Pages (from-to)1729-1733
Number of pages5
JournalTransplantation
Volume80
Issue number12
DOIs
StatePublished - Dec 1 2005

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Keywords

  • Citrulline
  • Dried blood spot
  • Intestine
  • Transplantation

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Hee, C. Y., Tuteja, S., Jang, I. M., Kleiner, G. I., Conanan, L., Gaynor, J. J., Kato, T., Levi, D. M., Nishida, S., Selvaggi, G., Gandia, C., Weppler, D., Esquenazi, V., Ruiz, P., Miller, J., & Tzakis, A. G. (2005). Utilization of dried blood spot citrulline level as a noninvasive method for monitoring graft function following intestinal transplantation. Transplantation, 80(12), 1729-1733. https://doi.org/10.1097/01.tp.0000188168.45003.63