Abstract
The objective of this study was to evaluate the utility of measuring IgM, IgG, and C-reactive protein (CRP) levels in rabbits with suspected Encephalitozoon cuniculi (ECUN) infection. Serum samples from 130 pet rabbits were submitted from veterinary clinics within the United States. Participating veterinarians completed a questionnaire that was used to classify rabbits as either non-ECUN suspect (n = 36) or suspected of having ECUN infection (n = 94). An enzyme-linked immunosorbent assay was used to detect IgG and IgM titers against ECUN. In addition, an immunoturbidimetric assay was used to detect CRP. In all, 50% of non-ECUN-suspect rabbits were IgG positive vs 95% of ECUN-suspect rabbits. When comparing the results from the 2 groups, higher titers of IgM and IgG and elevated CRP levels were observed in the ECUN-suspect group (P < 0.05). When examined alone, the positive predictive values of IgM, IgG, and CRP varied from 88% to 90%. The use of combinations of these tests decreased the sensitivity of the diagnostic panel but increased the specificity. -resulting in a positive predictive value ranging from 92% to 100%. These data suggest that the use of multiple immunological tests appears helpful in diagnosing ECUN in rabbit patients exhibiting clinical signs consistent with infection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 356-360 |
| Number of pages | 5 |
| Journal | Journal of Exotic Pet Medicine |
| Volume | 24 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jul 1 2015 |
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Keywords
- C-reactive protein
- ELISA
- Encephalitozoon cuniculi
- IgG
- IgM
- Serology
ASJC Scopus subject areas
- veterinary(all)
Cite this
Utility of IgM Titers With IgG and C-Reactive Protein Quantitation in the Diagnosis of Suspected Encephalitozoon Cuniculi Infection in Rabbits. / Cray, Carolyn; McKenny, Sarah; Perritt, Emily; Arheart, Kristopher.
In: Journal of Exotic Pet Medicine, Vol. 24, No. 3, 01.07.2015, p. 356-360.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Utility of IgM Titers With IgG and C-Reactive Protein Quantitation in the Diagnosis of Suspected Encephalitozoon Cuniculi Infection in Rabbits
AU - Cray, Carolyn
AU - McKenny, Sarah
AU - Perritt, Emily
AU - Arheart, Kristopher
PY - 2015/7/1
Y1 - 2015/7/1
N2 - The objective of this study was to evaluate the utility of measuring IgM, IgG, and C-reactive protein (CRP) levels in rabbits with suspected Encephalitozoon cuniculi (ECUN) infection. Serum samples from 130 pet rabbits were submitted from veterinary clinics within the United States. Participating veterinarians completed a questionnaire that was used to classify rabbits as either non-ECUN suspect (n = 36) or suspected of having ECUN infection (n = 94). An enzyme-linked immunosorbent assay was used to detect IgG and IgM titers against ECUN. In addition, an immunoturbidimetric assay was used to detect CRP. In all, 50% of non-ECUN-suspect rabbits were IgG positive vs 95% of ECUN-suspect rabbits. When comparing the results from the 2 groups, higher titers of IgM and IgG and elevated CRP levels were observed in the ECUN-suspect group (P < 0.05). When examined alone, the positive predictive values of IgM, IgG, and CRP varied from 88% to 90%. The use of combinations of these tests decreased the sensitivity of the diagnostic panel but increased the specificity. -resulting in a positive predictive value ranging from 92% to 100%. These data suggest that the use of multiple immunological tests appears helpful in diagnosing ECUN in rabbit patients exhibiting clinical signs consistent with infection.
AB - The objective of this study was to evaluate the utility of measuring IgM, IgG, and C-reactive protein (CRP) levels in rabbits with suspected Encephalitozoon cuniculi (ECUN) infection. Serum samples from 130 pet rabbits were submitted from veterinary clinics within the United States. Participating veterinarians completed a questionnaire that was used to classify rabbits as either non-ECUN suspect (n = 36) or suspected of having ECUN infection (n = 94). An enzyme-linked immunosorbent assay was used to detect IgG and IgM titers against ECUN. In addition, an immunoturbidimetric assay was used to detect CRP. In all, 50% of non-ECUN-suspect rabbits were IgG positive vs 95% of ECUN-suspect rabbits. When comparing the results from the 2 groups, higher titers of IgM and IgG and elevated CRP levels were observed in the ECUN-suspect group (P < 0.05). When examined alone, the positive predictive values of IgM, IgG, and CRP varied from 88% to 90%. The use of combinations of these tests decreased the sensitivity of the diagnostic panel but increased the specificity. -resulting in a positive predictive value ranging from 92% to 100%. These data suggest that the use of multiple immunological tests appears helpful in diagnosing ECUN in rabbit patients exhibiting clinical signs consistent with infection.
KW - C-reactive protein
KW - ELISA
KW - Encephalitozoon cuniculi
KW - IgG
KW - IgM
KW - Serology
UR - http://www.scopus.com/inward/record.url?scp=84940593914&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84940593914&partnerID=8YFLogxK
U2 - 10.1053/j.jepm.2015.06.017
DO - 10.1053/j.jepm.2015.06.017
M3 - Article
AN - SCOPUS:84940593914
VL - 24
SP - 356
EP - 360
JO - Journal of Exotic Pet Medicine
JF - Journal of Exotic Pet Medicine
SN - 1557-5063
IS - 3
ER -