Using hippocampal slices to study how aging alters ion regulation in brain tissue

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Abstract

Aging alters ion regulation in brain tissue. This article describes methods useful for studying such age-related changes in the rat hippocampal slice preparation. Topics considered include (a) selection of appropriate age groups of rats for aging studies, (b) a description of methods for preparing and maintaining hippocampal slices, (c) measurement of intracellular pH with the H+-sensitive dye carboxy-SNARF-1, and (d) measurement of extracellular pH and K+ with cation-selective microelectrodes.

Original languageEnglish
Pages (from-to)150-159
Number of pages10
JournalMethods: A Companion to Methods in Enzymology
Volume18
Issue number2
DOIs
StatePublished - Jun 1 1999

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Rats
Brain
Aging of materials
Ions
Tissue
Microelectrodes
Cations
Coloring Agents
Age Groups
carboxy-seminaphthorhodaminefluoride

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Using hippocampal slices to study how aging alters ion regulation in brain tissue. / Roberts, Eugene L.

In: Methods: A Companion to Methods in Enzymology, Vol. 18, No. 2, 01.06.1999, p. 150-159.

Research output: Contribution to journalArticle

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