Using hippocampal slices to study how aging alters ion regulation in brain tissue

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Aging alters ion regulation in brain tissue. This article describes methods useful for studying such age-related changes in the rat hippocampal slice preparation. Topics considered include (a) selection of appropriate age groups of rats for aging studies, (b) a description of methods for preparing and maintaining hippocampal slices, (c) measurement of intracellular pH with the H+-sensitive dye carboxy-SNARF-1, and (d) measurement of extracellular pH and K+ with cation-selective microelectrodes.

Original languageEnglish (US)
Pages (from-to)150-159
Number of pages10
JournalMethods: A Companion to Methods in Enzymology
Volume18
Issue number2
DOIs
StatePublished - Jun 1999

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Using hippocampal slices to study how aging alters ion regulation in brain tissue'. Together they form a unique fingerprint.

  • Cite this