Use of vivo-morpholinos for control of protein expression in the adult rat brain

Kathryn J. Reissner, Gregory C. Sartor, Elena M. Vazey, Thomas E. Dunn, Gary Aston-Jones, Peter W. Kalivas

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Vivo-morpholinos are commercially available morpholino oligomers with a terminal octa-guanidinium dendrimer for enhanced cell-permeability. Existing evidence from systemically delivered vivo-morpholinos indicate that genetic suppression can last from days to weeks without evidence of cellular toxicity. However, intravenously delivered vivo-morpholinos are ineffective at protein suppression in the brain, and no evidence is available regarding whether intracranially delivered vivo-morpholinos effectively reduce target protein levels, or do so without inducing neurotoxicity. Here we report examples in which in vivo microinjection of antisense vivo-morpholinos directed against three different targets (xCT, GLT1, orexin) in two different brain regions resulted in significant suppression of protein expression without neurotoxicity. Expression was significantly suppressed at six to seven days post-administration, but returned to baseline levels within fourteen days. These results indicate that direct intracranial administration of vivo-morpholinos provides an effective means by which to suppress protein expression in the brain for one to two weeks.

Original languageEnglish (US)
Pages (from-to)354-360
Number of pages7
JournalJournal of Neuroscience Methods
Volume203
Issue number2
DOIs
StatePublished - Jan 30 2012

Keywords

  • Antisense
  • GLT-1
  • Hypocretin
  • Lateral hypothalamus
  • Nucleus accumbens
  • Orexin
  • Vivo-morpholino
  • XCT

ASJC Scopus subject areas

  • Neuroscience(all)

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    Reissner, K. J., Sartor, G. C., Vazey, E. M., Dunn, T. E., Aston-Jones, G., & Kalivas, P. W. (2012). Use of vivo-morpholinos for control of protein expression in the adult rat brain. Journal of Neuroscience Methods, 203(2), 354-360. https://doi.org/10.1016/j.jneumeth.2011.10.009