Use of two MCF-7 cell variants to evaluate the growth regulatory potential of estrogen-induced products

N. E. Davidson, D. A. Bronzert, P. Chambon, Marc E Lippman, M. E. Lippman

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Two variants of the human estrogen-responsive breast cancer cell line MCF-7, were utilized to study the expression of an estrogen-induced gene, pS2, and an estrogen-induced M(r) 52,000 protein. One variant cell line, I13, is growth inhibited after chronic exposure to estrogen. Both the pS2 gene product and the M(r) 52,000 protein were produced at maximal levels at a time when I13 growth was inhibited by estrogen. The variant cell line, LY2, selected for its resistance to the growth-inhibitory effects of the antiestrogen, LY117018, grew normally in the presence of this drug, although both pS2 expression and M(r) 52,000 protein production were inhibited. These results confirm that the pS2 gene and M(r) 52,000 protein are estrogen-regulated elements, but the lack of correlation between their activities and variant cell growth suggests that they are not major autocrine growth-stimulatory agents.

Original languageEnglish
Pages (from-to)1904-1908
Number of pages5
JournalCancer Research
Volume46
Issue number4 II
StatePublished - Jul 30 1986
Externally publishedYes

Fingerprint

MCF-7 Cells
Estrogens
Growth
Cell Line
LY 117018
Genes
Proteins
Estrogen Receptor Modulators
Breast Neoplasms
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Davidson, N. E., Bronzert, D. A., Chambon, P., Lippman, M. E., & Lippman, M. E. (1986). Use of two MCF-7 cell variants to evaluate the growth regulatory potential of estrogen-induced products. Cancer Research, 46(4 II), 1904-1908.

Use of two MCF-7 cell variants to evaluate the growth regulatory potential of estrogen-induced products. / Davidson, N. E.; Bronzert, D. A.; Chambon, P.; Lippman, Marc E; Lippman, M. E.

In: Cancer Research, Vol. 46, No. 4 II, 30.07.1986, p. 1904-1908.

Research output: Contribution to journalArticle

Davidson, NE, Bronzert, DA, Chambon, P, Lippman, ME & Lippman, ME 1986, 'Use of two MCF-7 cell variants to evaluate the growth regulatory potential of estrogen-induced products', Cancer Research, vol. 46, no. 4 II, pp. 1904-1908.
Davidson NE, Bronzert DA, Chambon P, Lippman ME, Lippman ME. Use of two MCF-7 cell variants to evaluate the growth regulatory potential of estrogen-induced products. Cancer Research. 1986 Jul 30;46(4 II):1904-1908.
Davidson, N. E. ; Bronzert, D. A. ; Chambon, P. ; Lippman, Marc E ; Lippman, M. E. / Use of two MCF-7 cell variants to evaluate the growth regulatory potential of estrogen-induced products. In: Cancer Research. 1986 ; Vol. 46, No. 4 II. pp. 1904-1908.
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