Use of self-collected capillary blood samples for islet autoantibody screening in relatives

a feasibility and acceptability study

the Type 1 Diabetes TrialNet Study Group

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aims: To evaluate the feasibility of using self-collected capillary blood samples for islet autoantibody testing to identify risk in relatives of people with Type 1 diabetes. Methods: Participants were recruited via the observational TrialNet Pathway to Prevention study, which screens and monitors relatives of people with Type 1 diabetes for islet autoantibodies. Relatives were sent kits for capillary blood collection, with written instructions, an online instructional video link and a questionnaire. Sera from capillary blood samples were tested for autoantibodies to glutamic acid decarboxylase, islet antigen-2, insulin and zinc transporter 8. ‘Successful’ sample collection was defined as obtaining sufficient volume and quality to provide definitive autoantibody results, including confirmation of positive results by repeat assay. Results: In 240 relatives who returned samples, the median (range) age was 15.5 (1–49) years and 51% were male. Of these samples, 98% were sufficient for glutamic acid decarboxylase, islet antigen-2 and zinc transporter 8 autoantibody testing and 84% for insulin autoantibody testing and complete autoantibody screen. The upper 90% confidence bound for unsuccessful collection was 4.4% for glutamic acid decarboxylase, islet antigen-2 and/or zinc transporter 8 autoantibody assays, and 19.3% for insulin autoantibodies. Despite 43% of 220 questionnaire respondents finding capillary blood collection uncomfortable or painful, 82% preferred home self-collection of capillary blood samples compared with outpatient venepuncture (90% of those aged <8 years, 83% of those aged 9–18 years and 73% of those aged >18 years). The perceived difficulty of collecting capillary blood samples did not affect success rate. Conclusions: Self-collected capillary blood sampling offers a feasible alternative to venous sampling, with the potential to facilitate autoantibody screening for Type 1 diabetes risk.

Original languageEnglish (US)
Pages (from-to)934-937
Number of pages4
JournalDiabetic Medicine
Volume34
Issue number7
DOIs
StatePublished - Jul 1 2017

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Feasibility Studies
Autoantibodies
Glutamate Decarboxylase
Type 1 Diabetes Mellitus
Insulin
Antigens
Phlebotomy
Outpatients

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Use of self-collected capillary blood samples for islet autoantibody screening in relatives : a feasibility and acceptability study. / the Type 1 Diabetes TrialNet Study Group.

In: Diabetic Medicine, Vol. 34, No. 7, 01.07.2017, p. 934-937.

Research output: Contribution to journalArticle

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title = "Use of self-collected capillary blood samples for islet autoantibody screening in relatives: a feasibility and acceptability study",
abstract = "Aims: To evaluate the feasibility of using self-collected capillary blood samples for islet autoantibody testing to identify risk in relatives of people with Type 1 diabetes. Methods: Participants were recruited via the observational TrialNet Pathway to Prevention study, which screens and monitors relatives of people with Type 1 diabetes for islet autoantibodies. Relatives were sent kits for capillary blood collection, with written instructions, an online instructional video link and a questionnaire. Sera from capillary blood samples were tested for autoantibodies to glutamic acid decarboxylase, islet antigen-2, insulin and zinc transporter 8. ‘Successful’ sample collection was defined as obtaining sufficient volume and quality to provide definitive autoantibody results, including confirmation of positive results by repeat assay. Results: In 240 relatives who returned samples, the median (range) age was 15.5 (1–49) years and 51{\%} were male. Of these samples, 98{\%} were sufficient for glutamic acid decarboxylase, islet antigen-2 and zinc transporter 8 autoantibody testing and 84{\%} for insulin autoantibody testing and complete autoantibody screen. The upper 90{\%} confidence bound for unsuccessful collection was 4.4{\%} for glutamic acid decarboxylase, islet antigen-2 and/or zinc transporter 8 autoantibody assays, and 19.3{\%} for insulin autoantibodies. Despite 43{\%} of 220 questionnaire respondents finding capillary blood collection uncomfortable or painful, 82{\%} preferred home self-collection of capillary blood samples compared with outpatient venepuncture (90{\%} of those aged <8 years, 83{\%} of those aged 9–18 years and 73{\%} of those aged >18 years). The perceived difficulty of collecting capillary blood samples did not affect success rate. Conclusions: Self-collected capillary blood sampling offers a feasible alternative to venous sampling, with the potential to facilitate autoantibody screening for Type 1 diabetes risk.",
author = "{the Type 1 Diabetes TrialNet Study Group} and Y. Liu and Lisa Rafkin and D. Matheson and C. Henderson and D. Boulware and Besser, {R. E.J.} and C. Ferrara and L. Yu and Steck, {A. K.} and Bingley, {P. J.}",
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T1 - Use of self-collected capillary blood samples for islet autoantibody screening in relatives

T2 - a feasibility and acceptability study

AU - the Type 1 Diabetes TrialNet Study Group

AU - Liu, Y.

AU - Rafkin, Lisa

AU - Matheson, D.

AU - Henderson, C.

AU - Boulware, D.

AU - Besser, R. E.J.

AU - Ferrara, C.

AU - Yu, L.

AU - Steck, A. K.

AU - Bingley, P. J.

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Aims: To evaluate the feasibility of using self-collected capillary blood samples for islet autoantibody testing to identify risk in relatives of people with Type 1 diabetes. Methods: Participants were recruited via the observational TrialNet Pathway to Prevention study, which screens and monitors relatives of people with Type 1 diabetes for islet autoantibodies. Relatives were sent kits for capillary blood collection, with written instructions, an online instructional video link and a questionnaire. Sera from capillary blood samples were tested for autoantibodies to glutamic acid decarboxylase, islet antigen-2, insulin and zinc transporter 8. ‘Successful’ sample collection was defined as obtaining sufficient volume and quality to provide definitive autoantibody results, including confirmation of positive results by repeat assay. Results: In 240 relatives who returned samples, the median (range) age was 15.5 (1–49) years and 51% were male. Of these samples, 98% were sufficient for glutamic acid decarboxylase, islet antigen-2 and zinc transporter 8 autoantibody testing and 84% for insulin autoantibody testing and complete autoantibody screen. The upper 90% confidence bound for unsuccessful collection was 4.4% for glutamic acid decarboxylase, islet antigen-2 and/or zinc transporter 8 autoantibody assays, and 19.3% for insulin autoantibodies. Despite 43% of 220 questionnaire respondents finding capillary blood collection uncomfortable or painful, 82% preferred home self-collection of capillary blood samples compared with outpatient venepuncture (90% of those aged <8 years, 83% of those aged 9–18 years and 73% of those aged >18 years). The perceived difficulty of collecting capillary blood samples did not affect success rate. Conclusions: Self-collected capillary blood sampling offers a feasible alternative to venous sampling, with the potential to facilitate autoantibody screening for Type 1 diabetes risk.

AB - Aims: To evaluate the feasibility of using self-collected capillary blood samples for islet autoantibody testing to identify risk in relatives of people with Type 1 diabetes. Methods: Participants were recruited via the observational TrialNet Pathway to Prevention study, which screens and monitors relatives of people with Type 1 diabetes for islet autoantibodies. Relatives were sent kits for capillary blood collection, with written instructions, an online instructional video link and a questionnaire. Sera from capillary blood samples were tested for autoantibodies to glutamic acid decarboxylase, islet antigen-2, insulin and zinc transporter 8. ‘Successful’ sample collection was defined as obtaining sufficient volume and quality to provide definitive autoantibody results, including confirmation of positive results by repeat assay. Results: In 240 relatives who returned samples, the median (range) age was 15.5 (1–49) years and 51% were male. Of these samples, 98% were sufficient for glutamic acid decarboxylase, islet antigen-2 and zinc transporter 8 autoantibody testing and 84% for insulin autoantibody testing and complete autoantibody screen. The upper 90% confidence bound for unsuccessful collection was 4.4% for glutamic acid decarboxylase, islet antigen-2 and/or zinc transporter 8 autoantibody assays, and 19.3% for insulin autoantibodies. Despite 43% of 220 questionnaire respondents finding capillary blood collection uncomfortable or painful, 82% preferred home self-collection of capillary blood samples compared with outpatient venepuncture (90% of those aged <8 years, 83% of those aged 9–18 years and 73% of those aged >18 years). The perceived difficulty of collecting capillary blood samples did not affect success rate. Conclusions: Self-collected capillary blood sampling offers a feasible alternative to venous sampling, with the potential to facilitate autoantibody screening for Type 1 diabetes risk.

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